A single-step O-glycosylation of azidothymidine in bioreactor catalysed by filamentous fungi

Arruda, Evilanna Lima; Nunes, Elaine Sousa; Souza, Paula Letícia de Melo; Japiassu, Kamila Bohne; Carvalho, Thays Colletes de; Vaz, Boniek Gontijo; Liao, Luciano Morais; Oliveira, Valéria de

A single-step O-glycosylation of azidothymidine in bioreactor catalysed by filamentous fungi

Data

2016

Autores

Arruda, Evilanna Lima

Nunes, Elaine Sousa

Souza, Paula Letícia de Melo

Japiassu, Kamila Bohne

Carvalho, Thays Colletes de

Vaz, Boniek Gontijo

Liao, Luciano Morais

Oliveira, Valéria de

Resumo

The glycosylation of azidothymidine by Cunninghamella echinulata ATCC 9245 in a single step was achieved using whole cells in a stirred tank bioreactor (STR). Reaction conditions of 50 mg·mL−1 of 1 in PDSM medium at 28 °C for 168 h. After extraction with ethyl acetate and purification the derivative was identified with 3′-azido-3′-deoxythymidine 5′-O-α-d-glucopyranoside by Ultrahigh Resolution Mass Spectrometry and 1H, 13C, HSQC/HMBC.

Citação

ARRUDA, Evilanna L. et al. A single-step O-glycosylation of azidothymidine in bioreactor catalysed by filamentous fungi. Tetrahedron Letters, Amsterdam, v. 57, n. 39, p. 4392-4394, 2016. DOI: 10.1016/j.tetlet.2016.08.050. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S0040403916310784?via%3Dihub. Acesso em: 28 jun. 2023.