Preparation, characterization and in vitro antimicrobial activity of liposomal ceftazidime and cefepime against Pseudomonas aeruginosa strains

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dc.creatorTorres, Ieda Maria Sapateiro
dc.creatorBento, Etiene Barbosa
dc.creatorAlmeida, Larissa da Cunha
dc.creatorSá, Luisa Zaiden Carvalho Martins de
dc.creatorLima, Eliana Martins
dc.date.accessioned2019-02-01T13:01:44Z
dc.date.available2019-02-01T13:01:44Z
dc.date.issued2012-09
dc.description.abstractPseudomonas aeruginosa is an opportunistic microorganism with the ability to respond to a wide variety of environmental changes, exhibiting a high intrinsic resistance to a number of antimicrobial agents. This low susceptibility to antimicrobial substances is primarily due to the low permeability of its outer membrane, efflux mechanisms and the synthesis of enzymes that promote the degradation of these drugs. Cephalosporins, particularty ceftazidime and cefepime are effective against P. aeruginosa, however, its increasing resistance has limited the usage of these antibiotics. Encapsulating antimicrobial drugs into unilamellar liposomes is an approach that has been investigated in order to overcome microorganism resistance. In this study, antimicrobial activity of liposomal ceftazidime and cefepime against P. aeruginosa ATCC 27853 and P. aeruginosa SPM-1 was compared to that of the free drugs. Liposomal characterization included diameter, encapsulation efficiency and stability. Minimum Inhibitory Concentration (MIC) was determined for free and liposomal forms of both drugs. Minimum Bactericidal Concentration (MBC) was determined at concentrations 1, 2 and 4 times MIC. Average diameter of liposomes was 131.88 nm and encapsulation efficiency for cefepime and ceftazidime were 2.29% end 5.77%, respectively. Improved stability was obtained when liposome formulations were prepared with a 50% molar ratio for cholesterol in relation to the phospholipid. MIC for liposomal antibiotics for both drugs were 50% lower than that of the free drug, demonstrating that liposomal drug delivery systems may contribute to increase the antibacterial activity of these drugs.pt_BR
dc.identifier.citationTORRES, Ieda Maria Sapateiro et al. Preparation, characterization and in vitro antimicrobial activity of liposomal ceftazidime and cefepime against Pseudomonas aeruginosa strains. Brazilian Journal of Microbiology, São Paulo, v. 43, n. 3, p. 984-992, July/Sept. 2012.pt_BR
dc.identifier.issn1517-8382
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/16926
dc.language.isoengpt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentFaculdade de Farmácia - FF (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectPseudomonas aeruginosapt_BR
dc.subjectCephalosporinspt_BR
dc.subjectLiposomespt_BR
dc.titlePreparation, characterization and in vitro antimicrobial activity of liposomal ceftazidime and cefepime against Pseudomonas aeruginosa strainspt_BR
dc.typeArtigopt_BR

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