Selection of optimized candidate reference genes for qRT-PCR normalization in rice (Oryza sativa L.) during Magnaporthe oryzae infection and drought

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dc.creatorBevitori, Rosangela
dc.creatorOliveira, Marília Barros
dc.creatorSa, Maria Fatima Grossi de
dc.creatorLanna, Anna Cristina
dc.creatorSilveira, R. D. da
dc.creatorSilva, Silvana Petrofeza da
dc.date.accessioned2018-10-19T13:49:00Z
dc.date.available2018-10-19T13:49:00Z
dc.date.issued2014
dc.description.resumoDrought and rice blast disease caused by Magnaporthe oryzae are two of the most serious threats to global rice production. To explore the mechanisms underlying gene expression induced in rice by stresses, studies involving transcriptome analyses have been conducted over the past few years. Thus, it is crucial to have a reliable set of reference genes to normalize the expression levels of rice genes affected by different stresses. To identify potential reference genes for studies of the differential expression of target genes in rice under M. oryzae infection and drought conditions, the present study evaluated five housekeeping genes for the normalization of gene expression. The stability of the expression of these genes was assessed using the analytical software packages geNorm and NormFinder. For all samples analyzed, the stability rank was UBQ5 > GAPDH > eIF-4α > β-TUB > 18S rRNA. The data showed that the UBQ5, GAPDH, and eIF-4α genes are appropriate, high-performing reference genes and will be highly useful in future expression studies of fungal infections and drought in rice.pt_BR
dc.identifier.citationBEVITORI, R. et al. Selection of optimized candidate reference genes for qRT-PCR normalization in rice (Oryza sativa L.) during Magnaporthe oryzae infection and drought. Genetics and Molecular Research, Ribeirão Preto, v. 13, n. 4, p. 9795-9805, 2014.pt_BR
dc.identifier.doi10.4238/2014.November.27.7
dc.identifier.issne- 1676-5680
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/16218
dc.language.isoengpt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentInstituto de Ciências Biológicas - ICB (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectHousekeeping genespt_BR
dc.subjectNormalizationpt_BR
dc.subjectGene expressionpt_BR
dc.subjectReal-time quantitative reverse transcription polymerase chain reactionpt_BR
dc.subjectStable expressionpt_BR
dc.titleSelection of optimized candidate reference genes for qRT-PCR normalization in rice (Oryza sativa L.) during Magnaporthe oryzae infection and droughtpt_BR
dc.typeArtigopt_BR

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