The effect of crude ethanol extract and fractions of Hyptidendron canum (Pohl ex Benth.) Harley on the hepatopancreas of Oreochromis niloticus L
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2009-05
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Resumo
Hyptidendron canum (Pohl ex Benth.) Harley is a native tree of the Brazilian Savannah. The fish Oreochromis
niloticus L. was used as an experimental model to determine the bioactivity of the crude ethanol extract as
well as ethyl acetate, hexanic and chloroform fractions obtained from its leaves. The plant ethanol extract and
fractions were administered to the fish orally with their feed. Twenty four hours later, the fish were sacrificed
and their livers dissected, fixed in neutral formalin, embedded in paraffin and sectioned. Histological analyses
were performed using Masson’s trichrome and Haematoxylin-Eosin. Histochemical studies were performed
using Feulgen, PAS (Periodic Acid Schiff) and PAS + salivary amylase and Sudan IV stain. The qualitative
analysis of the material showed that both the crude ethanol extract and the fractions from H. canum induced
vasoactive activity, causing vasodilation and vascular congestion, and the hexanic fraction also caused an
apparent proliferation of capillaries. Hepatopancreas toxicity was evident through inflammatory processes.
Pancreatic (chloroform fraction) and hepatic alterations, hemorrhagic spots and necroses were observed in
fish treated with ethanol extract and fractions. This study is the first description of the biologic action of the
crude ethanol extract and the hexane, ethyl acetate and chloroform fractions in fish.
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Fish, Vasodilation, Nile tilapia, Savannah biome
Citação
FIUZA, Tatiana S. et al. The effect of crude ethanol extract and fractions of Hyptidendron canum (Polh ex Benth.) Harley on the hepatopancreas of Oreochromis niloticus L. Biological Research, Santiago, v. 42, n. 2, p. 153-162, May 2009.