Assessment of genotoxicity and cytotoxicity of “lixeira” (Curatella americana L.) using the prophage λ induction test (SOS inductest)
Carregando...
Data
2009-09
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
Curatella americana L., commonly known as “lixeira” in Brazil, has been used in folk medicine to treat
ulcers and inflammations. The purpose of the present work was to evaluate the cytotoxic and genotoxic
potential of the ethanolic extract of C. americana stem bark using the prophage λ induction test (SOS
inductest). To evaluate the cytotoxicity of this plant, after treatment with different concentrations of the
extract, Escherichia coli WP2s(λ) cultures were diluted in M9 buffer, inoculated into LB plates, and
incubated for 24 h at 37 °C. To assess genotoxicity, the lysogenic strain E. coli WP2s(λ) was treated
with different concentrations of the extract. Then, the lysogenic strain was added to the indicator strain
(RJF013), LB (1/2)(malt/amp) , seeded into plates with the matches, and incubated for 24 h at 37 °C. After this
period, the total number of colonies and the number of plaques were counted to evaluate C. americana
cytotoxicity and genotoxicity, respectively. Our results showed that although the extract of “lixeira” did
not modify the survival of bacteria (p > 0.05), it caused a significant increase in prophage λ induction,
especially at the higher concentrations (p<0.05). Therefore, we conclude that the ethanolic extract of
C. americana stem bark did not present cytotoxic effect, but some genotoxic potential was observed.
Descrição
Palavras-chave
Curatella americana, Lysogenic induction, Lixeira, Genotoxicity, Cytotoxicity, Curatella americana, Lixeira, Indução lisogênica, Genotoxicidade, Citotoxicidade
Citação
VILAR, Juliana Branstetter et al. Assessment of genotoxicity and cytotoxicity of "lixeira" (Curatella americana L.) using the prophage λ induction test (SOS inductest). Brazilian Journal of Pharmaceutical Sciences, São Paulo, v. 45, n. 3, p. 491-495, July/Sept. 2009.