Comparative proteomic analysis of histoplasma capsulatum yeast and mycelium reveals differential metabolic shifts and cell wall remodeling processes in the different morphotypes

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dc.creatorAlmeida, Marcos Abreu
dc.creatorBaeza, Lilian Cristiane
dc.creatorPaes, Rodrigo de Almeida
dc.creatorBailão, Alexandre Melo
dc.creatorBorges, Clayton Luiz
dc.creatorGuimarães, Allan Jefferson
dc.creatorSoares, Célia Maria Almeida
dc.creatorOliveira, Rosely Maria Zancopé
dc.date.accessioned2024-08-16T13:43:33Z
dc.date.available2024-08-16T13:43:33Z
dc.date.issued2021
dc.description.abstractHistoplasma capsulatum is a thermally dimorphic fungus distributed worldwide, but with the highest incidence in the Americas within specific geographic areas, such as the Mississippi River Valley and regions in Latin America. This fungus is the etiologic agent of histoplasmosis, an important life-threatening systemic mycosis. Dimorphism is an important feature for fungal survival in different environments and is related to the virulence of H. capsulatum, and essential to the establishment of infection. Proteomic profiles have made important contributions to the knowledge of metabolism and pathogenicity in several biological models. However, H. capsulatum proteome studies have been underexplored. In the present study, we report the first proteomic comparison between the mycelium and the yeast cells of H. capsulatum. Liquid chromatography coupled to mass spectrometry was used to evaluate the proteomic profile of the two phases of H. capsulatum growth, mycelium, and yeast. In summary, 214 and 225 proteins were only detected/or preferentially abundant in mycelium or yeast cells, respectively. In mycelium, enzymes related to the glycolytic pathway and to the alcoholic fermentation occurred in greater abundance, suggesting a higher use of anaerobic pathways for energy production. In yeast cells, proteins related to the tricarboxylic acid cycle and response to temperature stress were in high abundance. Proteins related to oxidative stress response or involved with cell wall metabolism were identified with differential abundance in both conditions. Proteomic data validation was performed by enzymatic activity determination, Western blot assays, or immunofluorescence microscopy. These experiments corroborated, directly or indirectly, the abundance of isocitrate lyase, 2-methylcitrate synthase, catalase B, and mannosyl-oligosaccharide-1,2-alpha-mannosidase in the mycelium and heat shock protein (HSP) 30, HSP60, glucosamine-fructose-6-phosphate aminotransferase, glucosamine-6-phosphate deaminase, and N-acetylglucosamine-phosphate mutase in yeast cells. The proteomic profile-associated functional classification analyses of proteins provided new and interesting information regarding the differences in metabolism between the two distinct growth forms of H. capsulatum.
dc.identifier.citationALMEIDA, Marcos Abreu et al. Comparative proteomic analysis of histoplasma capsulatum yeast and mycelium reveals differential metabolic shifts and cell wall remodeling processes in the different morphotypes. Frontiers in Microbiology, Lausanne, v. 12, e640931, 2021. DOI: 10.3389/fmicb.2021.640931. Disponível em: https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.640931/full. Acesso em: 8 ago. 2024.
dc.identifier.doi10.3389/fmicb.2021.640931
dc.identifier.issne- 1664-302X
dc.identifier.urihttp://repositorio.bc.ufg.br//handle/ri/25306
dc.language.isoeng
dc.publisher.countrySuica
dc.publisher.departmentInstituto de Ciências Biológicas - ICB (RMG)
dc.rightsAcesso Aberto
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectHistoplasma capsulatum
dc.subjectDimorphism
dc.subjectYeast
dc.subjectMycelium
dc.subjectProteomic analysis
dc.subjectFungal biology
dc.titleComparative proteomic analysis of histoplasma capsulatum yeast and mycelium reveals differential metabolic shifts and cell wall remodeling processes in the different morphotypes
dc.typeArtigo

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