Embryonic stem-like cells derived from in vitro produced bovine blastocysts

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dc.creatorFreitas, Érika Regina Leal de
dc.creatorSanches, Bruno Valente
dc.creatorGambarini, Maria Lúcia
dc.creatorOliveira Filho, Benedito Dias
dc.creatorGuillo, Lidia Andreu
dc.date.accessioned2018-02-16T12:52:21Z
dc.date.available2018-02-16T12:52:21Z
dc.date.issued2011-06
dc.description.abstractThe aim of this work was to study the derivation of bovine embryonic stem-like (ES-like) cells from the inner cell mass (ICM) of in vitro produced blastocysts. The ICMs were mechanically isolated and six out of seventeen (35%) ICMs could attach to a monolayer of murine embryonic fibroblasts (MEF).Ten days after, primary outgrowths were mechanically dissected into several small clumps and transferred to a new MEF layer. Cells were further propagated and passaged by physical dissociation over a 60 days period. The pluripotency of the bovine ES-like cells was confirmed by RT-PCR of Oct-4 and STAT-3 gene markers. The colonies were weakly stained for alkaline phosphatase and the mesoderm and endoderm differentiation gene markers such as GATA-4 and Flk-1, respectively, were not expressed. Embryoid bodies were spontaneously formed at the seventh passage. Results showed that bovine ES-like cells could be obtained and passaged by mechanical procedures from the fresh in vitro produced blastocysts.pt_BR
dc.identifier.citationFREITAS, Érika Regina Leal de; SANCHES, Bruno Valente; GAMBARINI, Maria Lúcia; OLIVEIRA FILHO, Benedito Dias; GUILLO, Lidia Andreu. Embryonic stem-like cells derived from in vitro produced bovine blastocysts. Brazilian Archives of Biology and Technology, Curitiba, v. 54, n. 3, p. 495-502, May/June 2011.pt_BR
dc.identifier.doi10.1590/S1516-89132011000300009
dc.identifier.issne- 1678-4324
dc.identifier.issn1516-8913
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/13650
dc.language.isoengpt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentEscola de Veterinária e Zootecnia - EVZ (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectBovine blastocystspt_BR
dc.subjectICM isolationpt_BR
dc.subjectEmbryonic stem cellspt_BR
dc.subjectGene markerspt_BR
dc.titleEmbryonic stem-like cells derived from in vitro produced bovine blastocystspt_BR
dc.typeArtigopt_BR

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