Expression of miR‑296‑5p as predictive marker for radiotherapy resistance in early‑stage laryngeal carcinoma

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dc.creatorMaia, Danielle
dc.creatorCarvalho, Ana Carolina de
dc.creatorHorst, Maria Aderuza
dc.creatorCarvalho, André Lopes
dc.creatorScapulatempo Neto, Cristovam
dc.creatorOliveira, Andre Luiz Vettore de
dc.date.accessioned2022-01-20T11:55:47Z
dc.date.available2022-01-20T11:55:47Z
dc.date.issued2015-08
dc.description.abstractPurpose: Definitive radiation therapy is the mainstay of treatment for early stage laryngeal squamous cell carcinoma (LSCC). However, up to 30% of the patients do not respond to radiotherapy. Unfortunately, we are unable to predict which tumors are likely to respond to radiation, and which will be resistant and persist. Therefore, the development of novel markers to predict response to radiotherapy is urgently needed. This study was designed to evaluate the expression pattern of microRNAs (miRNAs) in LSCC in order to identify markers capable of segregating radioresistant and radiosensitive tumors and to investigate the relationship between the expression of these miRNAs and the prognosis of LSCC. Methods: The expression profile of 667 miRNAs was determined in an initial screening of nine early-stage LSCC samples (5 radioresistant and 4 radiosensitive) using TaqMan Low-Density Array (TLDA). Real-time polymerase chain reactions were performed to validate the expression of selected miRNAs in an expanded LSCC cohort (20 radioresistant and 14 radiosensitive). The miRNA expression level was scored as high or low based on the median of the expression in the LSCC samples. Results: A comprehensive miRNA expression profiling enabled the identification of four miRNAs (miR-296-5p miR- 452, miR-183* and miR-200c) differentially expressed in radioresistant LSCC. Moreover, the analysis of additional 34 LSCC samples, confirmed the expression of miR-296-5p as significantly related to radioresistance (p = 0.002) as well as an association of this marker with recurrence (p = 0.025) in early stage laryngeal cancer. Conclusions: This study indicates that miR-296-5p expression is associated with resistance to radiotherapy and tumor recurrence in early stage LSCC, showing the feasibility of this marker as a novel prognostic factor for this malignance. Furthermore, miR-296-5p expression could be helpful in the identification of tumors resistant to radiotherapy; thus aiding the clinicians in the choice of the best therapeutic scheme to be used in each case.pt_BR
dc.identifier.citationMAIA, Danielle et al. Expression of miR-296-5p as predictive marker for radiotherapy resistance in early-stage laryngeal carcinoma. Journal of Translational Medicine, London, v. 13, p. 262-273, Aug. 2015.pt_BR
dc.identifier.doi10.1186/s12967-015-0621-y
dc.identifier.issne- 1479-5876
dc.identifier.issn1479-5876
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/20194
dc.language.isoengpt_BR
dc.publisher.countryGra-bretanhapt_BR
dc.publisher.departmentFaculdade de Nutrição - FANUT (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectEarly-stage laryngeal cancerspt_BR
dc.subjectmiR-296-5ppt_BR
dc.subjectMolecular markerpt_BR
dc.subjectMicroRNAspt_BR
dc.subjectRadioresistancept_BR
dc.titleExpression of miR‑296‑5p as predictive marker for radiotherapy resistance in early‑stage laryngeal carcinomapt_BR
dc.typeArtigopt_BR

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