Development and validation of a simple and rapid liquid chromatography method for the determination of genistein in skin permeation studies
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Data
2012
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Resumo
Genistein (GEN) has potential advantages for topical skin delivery, but no literature data are available
for its quantitation in different skin layers, such as the stratum corneum (SC). Therefore, a simple, rapid,
selective and sensitive bioanalytical method was developed and validated for GEN quantitation in porcine
skin samples following in vitro permeation studies. GEN was assayed by HPLC with UV-Vis detection
(270 nm) using 0.5% acetic acid in water–n-propanol–acetonitrile (50 : 2 : 48, v/v/v) as mobile phase (flow-
rate of 1.0 mL/min). Specificity was demonstrated since endogenous skin components did not interfere with
GEN peak. Standard analytical curve was linear over the concentration range (0.1–60 μg/mL) and the lower
limit of quantitation was determined for different skin layers (100 ng/mL). GEN recovery from skin layers
ranged from 95.57 to 97.57%. Permeation studies were carried out using an automated vertical diffusion cell
apparatus. No fluctuation on the amount of GEN retained in the SC was observed over time, but increasing
amounts of the drug were found in deeper layers of the skin. The method was reliable and reproducible for
the quantitation GEN in skin samples enabling the determination of the cutaneous penetration profile of this
drug in permeation experiments.
Descrição
Palavras-chave
Genistein, Skin layer, HPLC quantitation, Bioanalytical validation
Citação
MAIONE-SILVA, Lorena et al. Development and validation of a simple and rapid liquid chromatography method for the determination of genistein in skin permeation studies. Biological & Pharmaceutical Bulletin, Tokyo, v. 35, n. 11, p. 1986-1990, 2012.