Molecularly imprinted polymer-coated probe electrospray ionization mass spectrometry determines phorbol esters and deoxyphorbol metabolites in jatropha curcas leaves

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dc.creatorSilva, Lidya Cardozo da
dc.creatorCarvalho, Thays Colletes de
dc.creatorSilva, Igor Pereira da
dc.creatorMarana, Júlio César
dc.creatorLaviola, Bruno Galvêas
dc.creatorAbdelnur, Patrícia Verardi
dc.creatorVaz, Boniek Gontijo
dc.date.accessioned2023-07-31T13:26:24Z
dc.date.available2023-07-31T13:26:24Z
dc.date.issued2019
dc.description.abstractIn this study, a molecularly imprinted polymer-coated probe electrospray ionization mass spectrometry (MIPCPESI-MS) method was developed for detection of phorbol esters (PEs) and deoxyphorbol metabolites in Jatropha curcas leaves. Such an approach was established by sticking on a metallic needle a molecularly imprinted polymer to particularly design a MIP-coated probe for selective sampling and ionization of PEs and deoxyphorbol metabolites. By a subsequent application of a high voltage and methanol, as spray solvent, ESI was generated for direct and rapid analysis under ambient and open-air conditions. MIP-coated probe exhibited a high sampling capacity of the PEs and its metabolites in methanolic extracts of J. curcas leaves compared with the non-imprinted polymer (NIP)-coated probe. MIPCPESI-MS allowed the detection of phorbol 12,13-diacetate (PDA) from J. curcas leaves with minimal sample preparation, and with detection limit and quantification reaching 0.28 μg/mL and 0.92 μg/mL, respectively. Also, good linearity was obtained with R2 > 0.99 and precision and accuracy values between 4.06–13.49% and − 1.60 to − 15.26%, respectively. The current method was successfully applied to screening methanolic extracts of six different J. curcas leaf genotypes (three toxic and three non-toxic). PDA and three PE deoxyphorbol metabolites were identified only from toxic genotypes, in which PDA was determined with concentration ranging from 222.19 ± 23.55 to 528.23 ± 19.72 μg/g. All these findings support that the MIPCPESI-MS method developed here has a high potential for the analysis of PEs in plant extracts enabling differentiation of toxic and non-toxic genotypes earlier in the leaves.pt_BR
dc.identifier.citationSILVA, Lidya C. da. et al. Molecularly imprinted polymer-coated probe electrospray ionization mass spectrometry determines phorbol esters and deoxyphorbol metabolites in jatropha curcas leaves. Journal of the American Society for Mass Spectrometry, Washington, v. 30, n. 10, p. 2051-2059, 2019. DOI: 10.1007/s13361-019-02269-5. Disponível em: https://pubs.acs.org/doi/pdf/10.1007/s13361-019-02269-5. Acesso em: 28 jun. 2023.pt_BR
dc.identifier.doi10.1007/s13361-019-02269-5
dc.identifier.issn1044-0305
dc.identifier.issne- 1879-1123
dc.identifier.urihttps://pubs.acs.org/doi/pdf/10.1007/s13361-019-02269-5
dc.language.isoengpt_BR
dc.publisher.countryEstados unidospt_BR
dc.publisher.departmentInstituto de Química - IQ (RMG)pt_BR
dc.rightsAcesso Restritopt_BR
dc.subjectProbe electrospray ionization (PESI)pt_BR
dc.subjectMolecularly imprinted polymer (MIP)pt_BR
dc.subjectPhorbol esterspt_BR
dc.titleMolecularly imprinted polymer-coated probe electrospray ionization mass spectrometry determines phorbol esters and deoxyphorbol metabolites in jatropha curcas leavespt_BR
dc.typeArtigopt_BR

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