Caracterização do granulovírus SfGV e do seu efeito no controle da lagarta-do-cartucho (Spodoptera frugiperda)
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Data
2024-12-09
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Universidade Federal de Goias
Resumo
The larvae of Spodoptera frugiperda (J.E. Smith, 1797) (Lepidoptera: Noctuidae) are considered
one of the most damaging pests in Brazil, as they attack a wide range of crops, such as corn,
soybeans, and sorghum. One form of pest management that has been widely used over the years is
the use of baculoviruses as bioinsecticides, due to their high specificity, efficiency, and mortality
rates. During field trials in northeast Brazil, the company AgBiTech Controles Biológicos LTDA
has discovered a S. frugiperda caterpillar infected with a natural granulovirus, which is still
unknown. Thus, this study aimed to characterize this new baculovirus, named Spodoptera
frugiperda granulovirus (SfGV), and analyze its effect on the infection of larvae of this species. A
molecular characterization of the virus was conducted using the restriction fragment length
polymorphism (RFLP) method, with the enzymes Bam HI, Eco RI, and Pst I. Bioassays to test the
viral activity were performed in duplicates, and treatments with viral particles were diluted into
eight different doses. During the bioassay process, the symptoms of the larvae were analyzed and
photographed. According to the results, the baculovirus found is a previously undescribed
granulovirus that causes a type I infection syndrome, being a slow-kill granulovirus. The SfGV
was able to kill 100% of the infected larvae, according with a gradual increase in doses, showingan LT50 of 21 days. Therefore, SfGV demonstrated potential for use in control methods for S.
frugiperda.
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Baculovírus, Controle biológico, Sintomatologia, Pragas agrícolas, Baculovirus, Biological control, Symptomatology, Agricultural pests
Citação
PRADO, Samuel Araujo. Caracterização do granulovírus SfGV e do seu efeito no controle da lagarta-do-cartucho (Spodoptera frugiperda). 2024. 28 f. Trabalho de Conclusão de Curso
(Bacharelado em Ciências Biológicas) - Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, 2024.