Antiangiogenic potential of Jatropha curcas latex in the chick chorioallantoic membrane model
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2019
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AIMS: To perform a physicochemical and phytochemical characterization of Jatropha curcas latex and to investigate its antiangiogenic
potential.
METHODS: We performed an initial physicochemical characterization of J. curcas latex using thermal gravimetric analyses and Fourier
Transform Infrared spectroscopy. After that, phenols, tannins and flavonoids were quantified. Finally, the potential of J. curcas latex to
inhibit angiogenesis was evaluated using the chick chorioallantoic membrane model. Five groups of 20 fertilized chicken eggs each had
the chorioallantoic membrane exposed to the following solutions: (1) water, negative control; (2) dexamethasone, angiogenesis inhibitor;
(3) Regederm®, positive control; (4) 25% J. curcas latex diluted in water; (5) 50% J. curcas latex diluted in water; and (6) J. curcas crude
latex. Analysis of the newly-formed vascular net was made through captured images and quantification of the number of pixels. Histological
analyses were performed to evaluate the inflammation, neovascularization, and hyperemia parameters. The results were statically analyzed
with a significance level set at p<0.05.
RESULTS: Physicochemical characterization showed that J. curcas latex presented a low amount of cis-1.4-polyisoprene, which reduced its
elasticity and thermal stability. Phytochemical analyses of J. curcas latex identified a substantial amount of phenols, tannins, and flavonoids
(51.9%, 11.8%, and 0.07% respectively). Using a chick chorioallantoic membrane assay, we demonstrated the antiangiogenic potential of J.
curcas latex. The latex induced a decrease in the vascularization of the membranes when compared with neutral and positive controls (water
and Regederm®). However, when compared with the negative control (dexamethasone), higher J. curcas latex concentrations showed no
significant differences.
CONCLUSIONS: J. curcas latex showed low thermal stability, and consisted of phenols, tannins, and flavonoids, but little or no rubber.
Moreover, this latex demonstrated a significant antiangiogenic activity on a chick chorioallantoic membrane model. The combination of
antimutagenic, cytotoxic, antioxidant and antiangiogenic properties makes J. curcas latex a potential target for the development of new drugs.
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Physic nut, Pinhão-manso, Purging nut, Anticancer agents, Pinhão de purga, Membrana corioalantoide, Chorioallantoic membrane, Agentes antineoplásicos
Citação
ALMEIDA, Luciane Madureira et al. Antiangiogenic potential of Jatropha curcas latex in the chick chorioallantoic membrane model. Scientia Medica, Porto Alegre, v. 29, n. 1, e32157, 2019. DOI: 10.15448/1980-6108. 2019.1.32157. Disponível em: https://revistaseletronicas.pucrs.br/ojs/index.php/scientiamedica/article/view/32157. Acesso em: 5 maio 2023.