Disposable pipette extraction phase based on styrene-divinylbenzene/pernigraniline composite, applied for dexamethasone determination in synovial fluid by HPLC with UV detector

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dc.creatorSoares, Francielle Queiroz
dc.creatorAlvarenga, Bruna Faustino de
dc.creatorRuggiero, Marçal Antonio
dc.creatorColtro, Monise Cristina Ribeiro Casanova
dc.creatorLima, Eliana Martins
dc.creatorRabelo, Denilson
dc.creatorChaves, Andréa Rodrigues
dc.date.accessioned2023-05-31T12:50:31Z
dc.date.available2023-05-31T12:50:31Z
dc.date.issued2019
dc.description.abstractA reliable method using disposable pipette extraction (DPX) based on composite of pernigraniline and styrene– divinylbenzene (Sty–DVB) copolymer was applied to the analysis of dexamethasone in synovial fluid using highperformance liquid chromatography and ultraviolet (UV) detector (HPLC–UV). DPX variables, namely, number of draw/eject cycles for extraction or desorption, sample pH, volume, and desorption solvent, were optimized to establish the best sorption equilibrium and analysis time. The highest extraction efficiency value was obtained with 50 μL of synovial fluid mixed with 1950 μL of water, in five cycles of 300 μL of sampling, followed by liquid desorption of the drug with 300 μL of methanol in three cycles. The developed method demonstrated a linear response over the range from 10 to 100 ng/mL, with R2 = 0.993. The limit of quantification (LOQ) was 10 ng/mL. Based on the validation results, the proposed method can be a useful tool to detect dexamethasone levels in synovial fluid.pt_BR
dc.identifier.citationSOARES, Francielle Q. et al. Disposable pipette extraction phase based on styrene-divinylbenzene/pernigraniline composite, applied for dexamethasone determination in synovial fluid by HPLC with UV detector. Acta Chromatographica, Budapest, v. 31, n. 4, p. 250-254, 2019. DOI: 10.1556/1326.2018.00460. Disponível em: https://akjournals.com/view/journals/1326/31/4/article-p250.xml. Acesso em: 17 maio 2023.pt_BR
dc.identifier.doihttps://doi.org/10.1556/1326.2018.00460
dc.identifier.issne- 2083-5736
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/22645
dc.language.isoengpt_BR
dc.publisher.countryOutrospt_BR
dc.publisher.departmentInstituto de Química - IQ (RMG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectDexamethasonept_BR
dc.subjectDPX/HPLC–UVpt_BR
dc.subjectSynovial fluidpt_BR
dc.subjectPernigranilinept_BR
dc.titleDisposable pipette extraction phase based on styrene-divinylbenzene/pernigraniline composite, applied for dexamethasone determination in synovial fluid by HPLC with UV detectorpt_BR
dc.typeArtigopt_BR

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