Lipid dynamics in LPS-induced neuroinflammation by DESI-MS imaging
Nenhuma Miniatura disponível
Data
2019
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
It is well-established that bacterial lipopolysaccharides (LPS) can promote neuroinflammation through receptor
Toll-like 4 activation and induces sickness behavior in mice. This phenomenon triggers changes in membranes
lipid dynamics to promote the intracellular cell signaling. Desorption electrospray ionization mass spectrometry
(DESI-MS) is a powerful technique that can be used to image the distribution of lipids in the brain tissue directly.
In this work, we characterize the LPS-induced neuroinflammation and the lipid dynamics in C57BL/6 mice at 3
and 24 h after LPS injection. We have observed that intraperitoneal administration of LPS (5 mg/kg body weight)
induces sickness behavior and triggers a peripheral and cerebral increase of pro- and anti-inflammatory cytokine
levels after 3 h, but only IL-10 was upregulated after 24 h. Morphological analysis of hypothalamus, cortex and
hippocampus demonstrated that microglial activation was present after 24 h of LPS injection, but not at 3 h.
DESI-MS revealed a total of 14 lipids significantly altered after 3 and 24 h and as well as their neuroanatomical
distribution. Multivariate statistical analyzes have shown that ions associated with phosphatidylethanolamine
[PE(38:4)] and docosatetraenoic acid [FA (22:4)] could be used as biomarkers to distinguish samples from the
control or LPS treated groups. Finally, our data demonstrated that monitoring cerebral lipids dynamics and its
neuroanatomical distribution can be helpful to understand sickness behavior and microglial activation after LPS
administration.
Descrição
Palavras-chave
Lipopolysaccharide, Neuroinflammation, Sickness behavior, DESI-MS, Cerebral lipid dynamics
Citação
OLIVEIRA-LIMA, Onésia Cristina et al. Lipid dynamics in LPS-induced neuroinflammation by DESI-MS imaging. Brain, Behavior, and Immunity, Amsterdam, v. 79, p. 186-194, 2019. DOI: 10.1016/j.bbi.2019.01.029. Disponível em: https://www.sciencedirect.com/science/article/pii/S0889159118306391?via%3Dihub. Acesso em: 21 jun. 2023.