Inhibition of Leishmania amazonensis arginase by fucogalactan isolated from Agrocybe aegerita mushroom
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2018
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The inhibition of arginase from Leishmania spp. is considered a promising approach to the leishmaniasis treatment. In this study, the potential of a fucogalactan isolated from the medicinal mushroom Agrocybe aegerita was
evaluated against arginase (ARG) from Leishmania amazonensis. The polysaccharide was obtained via aqueous
extraction, and purified by freeze thawing and precipitation with Fehling solution. Its chemical structure was
established by monosaccharide composition, methylation analysis, partial acid hydrolysis, and NMR spectroscopy. The data indicated that it is a fucogalactan (FG-Aa; Mw = 13.8 kDa), having a (1→6)-linked α-D-Galp
main-chain partially substituted in O-2 by non-reducing end-units of α-L-Fucp. FG-Aa showed significant inhibitory activity on ARG with IC50potency of 5.82 ± 0.57 μM. The mechanism of ARG inhibition by the heterogalactan was the competitive type, with Kiof 1.54 ± 0.15 μM. This is the first report of an inhibitory activity
of arginase from L. amazonensis by biopolymers, which encourages us to investigate further polysaccharides as a
new class of ARG inhibitors.
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Agrocybe aegerita, Fucogalactan, Chemical structure, Leishmania amazonensis, Arginase, Competitive inhibitor
Citação
MOTOSHIMA, Renan Akio et al. Inhibition of Leishmania amazonensis arginase by fucogalactan isolated from Agrocybe aegerita mushroom. Carbohydrate Polymers, Amsterdam, v. 201, p. 532-538, 2018. DOI: 10.1016/j.carbpol.2018.08.109. Disponível em: https://www.sciencedirect.com/science/article/pii/S0144861718310191?via%3Dihub. Acesso em: 16 fev. 2024.