Comparison of ultrasound-assisted extraction and dynamic maceration over content of tagitinin C obtained from Tithonia diversifolia (Hemsl.) A. gray leaves using factorial design
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2017-06
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Background: Tithonia diversifolia belongs to the Asteraceae family. The
leaves of T. diversifolia have been studied lately because of the presence
of tagitinin C. Objective: Looking for an easy and inexpensive method to
extract tagitinin C from T. diversifolia leaves, this work aims to conduct a
screening to evaluate the influence of different experimental factors using
the dynamic maceration and ultrasound-assisted extraction methods with
2³ factorial design based on response surface methodology in enhancing
this chemical marker extraction. Materials and Methods: The experimental
factors were: extraction time (ET) of 30 and 60 minutes, solid: liquid ratio
(SLR) of 5 and 10 grams/grams and ethanolic strength (ES) 48 and 96%
(w/w). The experiments were done tripled. The content of tagitinin C in
each produced extract was quantified by HPLC method. Results: The
highest concentrations of tagitinin C obtained under the experimental
design were 0.53 mg/mL and 0.71 mg/mL, respectively for dynamic
maceration (DM) and ultrasound-assisted extraction (UAE) from Tithonia
diversifolia powdered leaves. For the UAE method, the main parameter
for higher contents of tagitinin C was the solid: liquid ratio, followed by
the ethanolic strength, and the extraction time was not significant for this
method. As for the DM method, all the parameters (SLR, ES, and ET) were
significant for a higher content of tagitinin C. Conclusion: Based on the
obtained results, it was revealed that the ultrasound-assisted extraction
was more effective than dynamic maceration for tagitinin C extraction from
T. diversifolia powdered leaves.
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Dynamic maceration, Factorial design, Response surface methodology, Tagitinin C, Ultrasound-assisted extraction
Citação
SILVA, Aline M. R. et al. Comparison of ultrasound-assisted extraction and dynamic maceration over
content of tagitinin C obtained from Tithonia diversifolia (Hemsl.) A. gray leaves
using factorial design. Pharmacognosy Magazine, Bangalore, v. 13, n. 50, p. 270-274, Apr./Jun. 2017. DOI: 10.4103/0973-1296.204555. Disponível em: https://phcog.com/article/view/13/50/270-274. Acesso em: 14 jun. 2023.