Control of microorganisms in vitro by different irrigant solutions
Data
2003
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
The aim of this study was to determine the minimum inhibitory concentration (MIC) and antimicrobial effectiveness by the direct
exposure test of 4 endodontic irrigants [1% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX), 1% calcium hydroxide (Ca(OH) 2;
prepared with 1 g of Ca(OH) 2 and 100 mL of sterile distilled water), a solution of Ca(OH) 2 + detergent (HCT20)] for S. aureus, E.
faecalis, P. aeruginosa, B. subtilis, C. albicans and a mixed culture. Microbial growth was analyzed by two methods: turbidity of the
culture medium that was confirmed by Gram stain and subculture in a specific nutrient broth. In the dilution test, NaOCl solution
showed MIC equal to 0.1% for S. aureus, E. faecalis, P. aeruginosa and C. albicans and equal to 1% for B. subtilis and the mixed
culture. CHX (2%) presented MIC equal to 0.000002% for S. aureus, 0.02% for E. faecalis, B. subtilis, C. albicans and the mixed
culture and 0.002% for P. aeruginosa. Ca(OH) 2 solution (1%) showed MIC greater than 1% for all the microorganisms except P.
aeruginosa for which it was equal to 1%. Calcium hydroxide solution + detergent showed MIC equal to 4.5 mL for S. aureus, P.
aeruginosa, B. subtilis, C. albicans and the mixed culture and greater than 4.5 mL for E. faecalis. In the direct exposure test, NaOCl
had better antimicrobial effectiveness for all microorganisms at all times. CHX (2%) was effective for S. aureus, E. faecalis and C.
albicans at all times, and ineffective for P. aeruginosa, B. subtilis and the mixed culture. The other solutions showed the worst results.
Descrição
Palavras-chave
Endodontic irrigants, Sodium hypochlorite, Chlorhexidine digluconate, Intracanal dressing
Citação
ESTRELA, Cyntia R. A. et al. Control of microorganisms in vitro by different irrigant solutions.. Brazilian Dental Journal, Ribeirão Preto, v. 14, n. 3, p. 187-192, 2003.