Avaliação da toxicidade aguda do extrato padronizado em psoraleno e bergapteno a partir de Brosimum gaudichaudii Trécul. usando os estágios embrio-larvais de zebrafish (Danio rerio)
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Data
2020-07-16
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Universidade Federal de Goiás
Resumo
Brosimum gaudichaudii Trécul. is a plant of the Moraceae family, often found in the savannahs of the states of Goiás, São Paulo and Mato Grosso, which contains furanocoumarins, mainly psoralen and bergaptene, responsible for skin repigmentation, so it can be used in the treatment of skin disease, like vitiligo. Herbal medicines must undergo toxicological studies in order to prove the safety of these medicines. This study aimed to evaluate the acute toxic effects (lethal and sublethal) of the standardized extract in psoralen and bergaptene from B. gaudichaudii Trécul. on the embryo-larval development of zebrafish. For this purpose, the acute toxicity test was performed with embryos and zebrafish larvae - Fish Embryo Toxicity Test (OECD 236, 2013). Twenty independent eggs were exposed in triplicate for each concentration of the extract and negative control (water from the maintenance system). The toxicity profile of the extract was time-dependent and concentration-dependent and the sublethal effects observed were: pericardial edema, yolk sac edema, lack of body pigmentation and inhibition of hatching. Therefore, it was possible to conclude that the extract is embryotoxic for zebrafish, with significant lethal effects from the concentration 0.005 µg / mL in 72 hours of exposure, in addition to relevant malformations throughout the period of exposure.
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Brosimum gaudichaudii, Métodos alternativos, Mortalidade, Malformações, Alternative methods, Mortality, Malformations
Citação
SILVA, Hugo Santiago Francisco da. Avaliação da toxicidade aguda do extrato padronizado em psoraleno e bergapteno a partir de Brosimum gaudichaudii Trécul. usando os estágios embrio-larvais de zebrafish (Danio rerio). 2020. 33 f. Trabalho de Conclusão de Curso (Graduação) - Faculdade de Farmácia, Universidade Federal de Goiás, Goiânia, 2020.