A new method for the determination of creatinine in urine samples based on disposable pipette extraction
| dc.creator | Fernandes, Aline Rosa | |
| dc.creator | Souza, Paulo Sérgio de | |
| dc.creator | Oliveira, Anselmo Elcana de | |
| dc.creator | Chaves, Andréa Rodrigues | |
| dc.date.accessioned | 2023-05-19T21:34:51Z | |
| dc.date.available | 2023-05-19T21:34:51Z | |
| dc.date.issued | 2018 | |
| dc.description.resumo | A method based on disposable pipette extraction (DPX) was successfully applied to creatinine determination in urine samples analysis using liquid chromatography with ultraviolet spectrophotometric detection (DPX/LC-UV). DPX variables, number of draw/eject cycles, sample pH, and type of the desorption solvent, were employed in a factorial experimental design to optimize the sorption equilibrium and time analysis. Among the evaluated DPX variables, the highest extraction efficiency was obtained with 500 µL of urine sample mixed with 1 mL of borate solution (pH 9) with one draw/eject cycle followed by liquid desorption of 1 mL of methanol in seven draw/eject cycles. The developed DPX/LC-UV method showed a linear response from the limit of quantification of 0.317 to 3.390 g L-1 with r2 = 0.996 and inter-day precision with a coefficient of variation below 8.8%. Based on these results, the proposed method can be a useful tool for determining the creatinine levels in urine samples. | pt_BR |
| dc.identifier.citation | FERNANDES, Aline R.; SOUZA, Paulo S. de; OLIVEIRA Anselmo E. de; CHAVES ,Andréa R. A new method for the determination of creatinine in urine samples based on disposable pipette extraction. Journal of the Brazilian Chemical Society, Campinas, v. 29, n. 4, p. 695-700, 2017. Disponível em: https://jbcs.sbq.org.br/default.asp?ed=265. Acesso em: 17 maio 2023. | pt_BR |
| dc.identifier.doi | http://dx.doi.org/http://dx.doi.org/10.21577/0103-5053.20170187 | |
| dc.identifier.issn | e- 1678-4790 | |
| dc.identifier.issn | 0103-5053 | |
| dc.identifier.uri | http://repositorio.bc.ufg.br/handle/ri/22580 | |
| dc.language.iso | eng | pt_BR |
| dc.publisher.country | Brasil | pt_BR |
| dc.publisher.department | Instituto de Química - IQ (RMG) | pt_BR |
| dc.rights | Acesso Aberto | pt_BR |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
| dc.subject | Creatinine | pt_BR |
| dc.subject | Disposable pipette extraction | pt_BR |
| dc.subject | Urine sample | pt_BR |
| dc.subject | Liquid chromatography | pt_BR |
| dc.title | A new method for the determination of creatinine in urine samples based on disposable pipette extraction | pt_BR |
| dc.type | Artigo | pt_BR |