Isolation and quantitative HPLC-PDA analysis of lupeol in phytopharmaceutical intermediate products from Vernonanthura ferruginea (Less.) H. Rob.

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dc.creatorSilva, Ezequiane Machado da
dc.creatorFreitas, Sabrine Lemes
dc.creatorMartins, Frederico Severino
dc.creatorCouto, Renê Oliveira
dc.creatorPinto, Mariana Viana
dc.creatorPaula, José Realino de
dc.creatorConceição, Edemilson Cardoso da
dc.creatorBara, Maria Teresa Freitas
dc.date.accessioned2018-09-21T15:03:09Z
dc.date.available2018-09-21T15:03:09Z
dc.date.issued2012
dc.descriptionPrior to obtain a standardized dried extract from V. ferruginea, lupeol was first time isolated from leaves and used as chemical maker. An analytical method using HPLC-PDA for lupeol determination in V. ferruginea intermediate products was developed using a C8 reverse-phase column, acetonitrile-acetic acid (99.99:0.01, v/v) as mobile phase at 0.8 mL min -1 , oven temperature at 23‐25 °C, sample injection volume at 30 μL and detection at 210 nm. The method presented linearity from 10 to 160 μg mL -1 , accuracy, precision, robustness and suitable sensitivity proving to be a useful tool to the obtainment process of lupeol standardized dried extracts of V. ferruginea.pt_BR
dc.identifier.citationOLIVEIRA, Ezequiane Machado Silva et al. Isolation and quantitative HPLC-PDA analysis of lupeol in phytopharmaceutical intermediate products from Vernonanthura ferruginea (Less.) H. Rob. Química Nova, São Paulo, v. 35, n. 5, p. 1041-1045, 2012.pt_BR
dc.identifier.doi10.1590/S0100-40422012000500032
dc.identifier.issn0100-4042
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/16003
dc.language.isoengpt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentFaculdade de Farmácia - FF (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectQuality controlpt_BR
dc.subjectStandardized dried extractpt_BR
dc.subjectTriterpenept_BR
dc.titleIsolation and quantitative HPLC-PDA analysis of lupeol in phytopharmaceutical intermediate products from Vernonanthura ferruginea (Less.) H. Rob.pt_BR
dc.typeArtigopt_BR

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