The ruthenium complex cis-(dichloro)tetrammineruthenium(III) chloride induces apoptosis and damages DNA in murine sarcoma 180 cells

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dc.creatorLima, Aliny Pereira de
dc.creatorPereira, Flávia de Castro
dc.creatorVilanova-Costa, Cesar Augusto Sam Tiago
dc.creatorRibeiro, Alessandra de Santana Braga Barbosa
dc.creatorPavanin, Luiz Alfredo
dc.creatorSantos, Wagner Batista dos
dc.creatorLacerda, Elisângela de Paula Silveira
dc.date.accessioned2018-08-28T11:45:00Z
dc.date.available2018-08-28T11:45:00Z
dc.date.issued2010-09
dc.description.abstractRuthenium(III) complexes are increasingly attracting the interest of researchers due to their promising pharmacological properties. Recently, we reported that the cis-(dichloro)tetrammineruthenium(III) chloride compound has cytotoxic effects on murine sarcoma 180 (S-180) cells. In an effort to understand the mechanism responsible for their cytotoxicity, study we investigated the genotoxicity, cell cycle distribution and induction of apoptosis caused by cis- (dichloro)tetrammineruthenium(III) chloride in S-180 tumour cells. cis-(dichloro)tetrammineruthenium(III) chloride treatment induced signifi cant DNA damage in S-180 cells, as detected by the alkaline comet assay. In the cell cycle analysis, cis-(dichloro)tetrammineruthenium(III) chloride caused an increase in the number of cells in G1 phase, accompanied by a decrease in the S and G2 phases after 24 h of treatment. In contrast, the cell cycle distribution of S-180 cells treated with cis-(dichloro)tetrammineruthenium(III) chloride for 48 h showed a concentration-dependent increase in the sub-G1 phase (indicating apoptosis), with a corresponding decrease in cells in the G1, S and G2 phases. In addition, cis-(dichloro)tetrammineruthenium(III) chloride treatment induced apoptosis in a time-dependent manner, as observed by the increased numbers of annexin V-positive cells. Taken together, these fi ndings strongly demonstrate that DNA damage, cell cycle changes and apoptosis may correlate with the cytotoxic effects of cis-(dichloro)tetramm- ineruthenium(III) chloride on S-180 cells.pt_BR
dc.identifier.citationLIMA, Aliny Pereira de; PEREIRA, Flavia de Castro; VILANOVA-COSTA, Cesar Augusto Sam Tiago; RIBEIRO, Alessandra de Santana Braga Barbosa; PAVANIN, Luiz Alfredo; SANTOS, Wagner Batista dos; SILVEIRA-LACERDA, Elisângela de Paula. The ruthenium complex cis-(dichloro)tetrammineruthenium(III) chloride induces apoptosis and damages DNA in murine sarcoma 180 (S-180) cells. Journal of Biosciences, Berlim, v. 35, n. 3, p. 371-378, Sept. 2010.pt_BR
dc.identifier.issn0250-5991
dc.identifier.issne- 0973-7138
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/15761
dc.language.isoengpt_BR
dc.publisher.countryAlemanhapt_BR
dc.publisher.departmentInstituto de Ciências Biológicas - ICB (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectApoptosispt_BR
dc.subjectS-180pt_BR
dc.subjectCis-(dichloro)tetrammineruthenium(III) chloridept_BR
dc.subjectCell cyclept_BR
dc.subjectDNA damagept_BR
dc.titleThe ruthenium complex cis-(dichloro)tetrammineruthenium(III) chloride induces apoptosis and damages DNA in murine sarcoma 180 cellspt_BR
dc.typeArtigopt_BR

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