Bioassay-guided fractionation of Siparuna glycycarpa n-butanol extract with inhibitory activity against influenza A(H1N1)pdm09 virus by centrifugal partition chromatography (CPC)
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Data
2022-01-08
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Resumo
Siparuna glycycarpa occurs in the Amazon region, and some species of this genus are
used in Brazilian folk medicine. A recent study showed the inhibitory effect of this species against
influenza A(H1N1)pdm09 virus, and in order to acquire active fractions, a polar solvent system
n-butanol-methanol-water (9:1:10, v/v) was selected and used for bioassay-guided fractionation of
n-butanol extract by centrifugal partition chromatography (CPC). The upper phase was used as
stationary phase and the lower phase as mobile (descending mode). Among the collected fractions,
the ones coded SGA, SGC, SGD, and SGO showed the highest antiviral inhibition levels (above 74%)
at 100 µg·mL−1 after 24 h of infection. The bioactive fractions chemical profiles were investigated by
LC-HRMS/MS data in positive and negative ionization modes exploring the Global Natural Products
Social Molecular Networking (GNPS) platform to build a molecular network. Benzylisoquinoline
alkaloids were annotated in the fractions coded SGA, SGC, and SGD collected during elution step.
Aporphine alkaloids, O-glycosylated flavonoids, and dihydrochalcones in SGO were acquired with
the change of mobile phase from lower aqueous to upper organic. Benzylisoquinolinic and aporphine
alkaloids as well as glycosylated flavonoids were annotated in the most bioactive fractions suggesting
this group of compounds as responsible for antiviral activity.
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Centrifugal partition chromatography, Influenza virus, Mass spectrometry, Off-line LCMS/MS, Siparunaceae, Solvent system
Citação
LEAL, Carla Monteiro et al. Bioassay-guided fractionation of Siparuna glycycarpa n-butanol extract with inhibitory activity against influenza A(H1N1)pdm09 virus by centrifugal partition chromatography (CPC). Molecules, Basel, v. 27, e399, 2022. DOI: 10.3390/molecules27020399. Disponível em: https://www.mdpi.com/1420-3049/27/2/399. Acesso em: 21 jun. 2023.