The interaction of lipid-liganded gold clusters (Aurora ™) with lipid bilayers

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dc.creatorSot Sanz, Jesus
dc.creatorMendanha Neto, Sebastião Antônio
dc.creatorBusto, Jon V.
dc.creatorGarcía Arribas, Aritz B.
dc.creatorShengrong, Li
dc.creatorBurgess, Stephen W.
dc.creatorShaw, Walt A.
dc.creatorGil-Carton, David
dc.creatorGoñi, Félix M.
dc.creatorAlonso, Alicia
dc.date.accessioned2024-07-15T11:36:16Z
dc.date.available2024-07-15T11:36:16Z
dc.date.issued2019
dc.description.abstractLipid bilayers of different phospholipid compositions have been prepared, in the form of vesicles, or of supported lipid bilayers, and doped with Aurora™ at 0.1 mol%. Aurora™ consists of an Au55 gold nanoparticle (about 1.4 nm in diameter) capped with triphenylphosphine ligands and a single diglyceride (distearoyl glycerol) ligand. Gold nanoparticles have been incorporated in the past inside liposomes, or grafted onto their surfaces, with diagnostic or therapeutic aims. Including the gold nanoparticles in a stable form within the lipid bilayers has serious technical difficulties. We have tested the hypothesis that, because of the diglyceride ligand, Aurora™ would allow the easy incorporation of gold nanoclusters into cell membranes or lipid bilayers. Our results show that Aurora™ readily incorporates into lipid bilayers, particularly when they are in the fluid phase, i.e. the state in which cell membranes exist. Calorimetric, fluorescence polarization or fluorescence confocal microscopy concur in showing that bilayer-embedded Aurora™hardly changes the physical properties of the bilayers, nor does it perturb the phase equilibrium in lipid mixtures giving rise to lateral phase separation in the plane of the membrane. Atomic force microscopy shows, in fluid bilayers, well-resolved particles, 1.2–2.9 nm in height, that are interpreted as single Aurora™conjugates. Cryo-transmission electron microscopy allows the clear observation of lipid bilayers with an enhanced contrast due to the Aurora™ gold nanoparticles; the single particles can be resolved at high magnification. Our studies support the applicability of Aurora™ as a membrane-friendly form of nano-gold particles for biological research or clinical applications.
dc.identifier.citationSOT, Jesús et al. The interaction of lipid-liganded gold clusters (Aurora -) with lipid bilayers. Chemistry and Physics of Lipids, Amsterdam, v. 218, p. 40-46, 2019. DOI: 10.1016/j.chemphyslip.2018.11.007. Disponível em: https://www.sciencedirect.com/science/article/pii/S000930841830238X. Acesso em: 26 maio 2023.
dc.identifier.doi10.1016/j.chemphyslip.2018.11.007
dc.identifier.issn0009-3084
dc.identifier.issne- 1873-2941
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S000930841830238X
dc.language.isoeng
dc.publisher.countryHolanda
dc.publisher.departmentInstituto de Física - IF (RMG)
dc.rightsAcesso Restrito
dc.subjectLipid-liganded gold clusters
dc.subjectAurora™
dc.subjectLipid bilayers
dc.subjectLipid phases
dc.subjectAtomic force microscopy
dc.titleThe interaction of lipid-liganded gold clusters (Aurora ™) with lipid bilayers
dc.typeArtigo

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