A cerumenolomic approach to bovine trypanosomosis diagnosis
Nenhuma Miniatura disponível
Data
2022
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
Introduction
Trypanosomiasis caused by Trypanosoma vivax (T. vivax, subgenus Duttonella) is a burden disease in bovines that induces losses of billions of dollars in livestock activity worldwide. To control the disease, the first step is identifying the infected animals at early stages. However, convention tools for animal infection detection by T. vivax present some challenges, facilitating the spread of the pathogenesis.
Objectives
This work aims to develop a new procedure to identify infected bovines by T. vivax using cerumen (earwax) in a volatilomic approach, here named cerumenolomic, which is performed in an easy, quick, accurate, and non-invasive manner.
Methods
Seventy-eight earwax samples from Brazilian Curraleiro Pé-Duro calves were collected in a longitudinal study protocol during health and inoculated stages. The samples were analyzed using Headspace/Gas Chromatography–Mass Spectrometry followed by multivariate analysis approaches.
Results
The cerumen analyses lead to the identification of a broad spectrum of volatile organic metabolites (VOMs), of which 20 VOMs can discriminate between healthy and infected calves (AUC = 0.991, sensitivity = 0.967, specificity = 1.000). Furthermore, 13 VOMs can indicate a pattern of discrimination between the acute and chronic phases of the T. vivax infection in the animals (AUC = 0.989, sensitivity = 0.944, specificity = 1.000).
Conclusion
The cerumen volatile metabolites present alterations in their occurrence during the T.vivax infection, which may lead to identifying the infection in the first weeks of inoculation and discriminating between the acute and chronic phases of the illness. These results may be a breakthrough to avoid the T. vivax outbreak and provide a faster clinical approach to the animal.
Descrição
Palavras-chave
Genetic algorithm, Receiver operating curve, Cattle, VOCs, HS/GC–MS
Citação
BARBOSA, João Marcos G. et al. A cerumenolomic approach to bovine trypanosomosis diagnosis. Metabolomics, Dordrecht, v. 18, e42, 2022. DOI: 10.1007/s11306-022-01901-y. Disponível em: https://link.springer.com/article/10.1007/s11306-022-01901-y. Acesso em: 14 jun. 2023.