Patógenos periodontais: comparação entre culrura bacteriana e PCR em tempo real para teste de diagnóstico
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Data
2010
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Resumo
The correct distinguishment of microorganisms involved in
the periodontal disease pathogen, it is important in the under-
standing of its progression and adequate treatment planning.
Considering this fact, some molecular methods of identification
and quantification were developed and are extremely sensitive
and precise in the characterization of different bacteria species.
The present study aimed to realize a literature review, including
studies that realized a comparative analysis between bacterial
culture and real time PCR methods in the identification of patho-
gens. The bacterial culture method can possibly identify new
microorganisms and realize antibiotics sensitivity tests. The real time PCR is a microbiologic test that identifies and quantifies
bacterial species, through gene amplification of predetermined
DNA fragments, with high sensitivity and specificity, and need
a shorter operation time of the operator when compared to the
bacterial culture method. In this way, to determine a specific
diagnostic test, should be considered not only its precision in
the identification of microorganisms, but the cost-benefit rela-
tionship as well.
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Palavras-chave
Meios de cultura, Periodontite, Reação em cadeia da polimerase (PCR), Culture media, Polymerase Chain Reaction (PCR), Periodontitis
Citação
BEDRAN, Telma B. L. et al. Patógenos periodontais: comparação entre culrura bacteriana e PCR em tempo real para teste de diagnóstico. Robrac: revista odontologica do Brasil Central, Goiânia, v. 19, n. 50, p. 187-191, 2010.