Hydrodynamic injection on electrophoresis microchips using an electronic micropipette

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dc.creatorMoreira, Ellen Flávia
dc.creatorSantos, Gabriel Rodrigo Alexandre dos
dc.creatorLobo Júnior, Eulício de Oliveira
dc.creatorMartins, Kariolanda Cristina de Andrade Rezende
dc.creatorColtro, Wendell Karlos Tomazelli
dc.date.accessioned2024-11-11T17:11:55Z
dc.date.available2024-11-11T17:11:55Z
dc.date.issued2017-01
dc.description.abstractHere we report for the first time the use of an electronic micropipette as hydrodynamic (HD) injector for microchip electrophoresis (ME) devices. The micropipette was directly coupled to a PDMS device, which had been fabricated in a simple cross format with two auxiliary channels for sample volume splitting. Sample flow during the injection procedure was controlled in automatic dispenser mode using a volume of 0.6 µL. Channel width and device configuration were optimized and the best results were achieved using a simple cross layout containing two auxiliary channels with 300 µm width for sample splitting. The performance of the HD injector was evaluated using a model mixture of high-mobility cationic species. The results obtained were compared to the data obtained via electrokinetic (EK) injection. Overall, the HD provided better analytical performance in terms of resolution and injection-to-injection repeatability. The relative standard deviation (RSD) values for peak intensities were lower than 5% (n=10) when the micropipette was employed. In comparison with EK injection, the use of the proposed HD injector revealed an unbiased profile for a mixture containing K+ and Li+(300 µmol L−1 each) over various buffer concentrations. For EK injection, the peak areas decreased from 2.92 ± 0.20–0.72 ± 0.14 V s for K+ and from 1.30 ± 0.10–0.38 ± 0.10 V s for Li+ when the running buffer increased from 20 to 50 mmol L−1. For HD injection, the peak areas for K+ and Li+ exhibited average values of 2.48±0.07 and 2.10±0.06 V s, respectively. The limits of detection (LDs) for K+, Na+ and Li+ ranged from 18 to 23 µmol L−1. HD injection through an electronic micropipette allows to automatically dispense a bias-free amount of sample inside microchannels with acceptable repeatability. The proposed approach also exhibited instrumental simplicity, portability and minimal microfabrication requirements.
dc.identifier.citationGABRIEL, Ellen F. M. et al. Hydrodynamic injection on electrophoresis microchips using an electronic micropipette. Talanta, (Oxford), v. 162, p. 19-23, 2017. DOI: 10.1016/j.talanta.2016.09.046. Disponível em: https://www.sciencedirect.com/science/article/pii/S0039914016307172?via%3Dihub. Acesso em: 8 ago. 2024.
dc.identifier.doi10.1016/j.talanta.2016.09.046
dc.identifier.issn0039-9140
dc.identifier.issne- 1873-3573
dc.identifier.urihttp://repositorio.bc.ufg.br//handle/ri/25869
dc.language.isoeng
dc.publisher.countryGra-bretanha
dc.publisher.departmentInstituto de Química - IQ (RMG)
dc.rightsAcesso Aberto
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectAutomatic dispensing
dc.subjectBias-free sample injection
dc.subjectMicrofluidic transport
dc.subjectPortable instrumentation
dc.titleHydrodynamic injection on electrophoresis microchips using an electronic micropipette
dc.typeArtigo

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