Determination of ranitidine in human plasma by SPE and ESI-LC-MS/MS for use in bioequivalence studies
| dc.creator | Bellorio, Karini Bruno | |
| dc.creator | Alves, Maria Isabel Ribeiro | |
| dc.creator | Antoniosi Filho, Nelson Roberto | |
| dc.date.accessioned | 2018-04-06T14:48:45Z | |
| dc.date.available | 2018-04-06T14:48:45Z | |
| dc.date.issued | 2013 | |
| dc.description.resumo | A method for determining ranitidine in human plasma by ESI-LC-MS/MS was validated, using propranolol as internal standard. e extraction method used was solid phase extraction (SPE). Chromatographic separation was performed in a Chromolith C18 (50 mm × 4.6 mm i.d.) analytical column, which provided good separation of ranitidine and propranolol peaks with an analysis time of 2.5 minutes. Extraction yields of 94.4% for ranitidine and 89.4% for the internal standard were obtained. e lower limit of quantication (LLO) was 3.00 ng/mL, and limit of detection (LOD) was 0.05 ng/mL, with linearity ranging from 3.00 to 500 ng/mL. e results, thus, showed that this method is suitable for application in bioequivalence studies of ranitidine in human plasma. | pt_BR |
| dc.identifier.citation | BELLORIO, Karini B.; ALVES, Maria Isabel R.; Antoniosi Filho, Nelson R. Determination of ranitidine in human plasma by SPE and ESI-LC-MS/MS for use in bioequivalence studies. ISRN Chromatography, London, v. 2013, p. 1-7, 2013. | pt_BR |
| dc.identifier.doi | 10.1155/2013/484592 | |
| dc.identifier.uri | http://repositorio.bc.ufg.br/handle/ri/14391 | |
| dc.language.iso | eng | pt_BR |
| dc.publisher.country | Brasil | pt_BR |
| dc.publisher.department | Instituto de Química - IQ (RG) | pt_BR |
| dc.rights | Acesso Aberto | pt_BR |
| dc.title | Determination of ranitidine in human plasma by SPE and ESI-LC-MS/MS for use in bioequivalence studies | pt_BR |
| dc.type | Artigo | pt_BR |