Effects of enhanced-efficiency nitrogen fertilizers on soil microbial biomass and respiration in tropical soil under upland rice cultivation

Abstract

While over-use of N fertilizers can suppress microbial biomass, application of urease inhibitors is known to be a potential way to rebuilt microbial diversity and improve soil functions. However, the hypothesis of this study is that the application of N fertilizers regardless of the source would increase soil microbial biomass and reduce soil respiration. A two-year field experiment was conducted to assess the effects of enhanced-efficiency N sources on soil microbial biomass, and soil respiration. The experiment was set up in a randomized block design in a 3 × 4 + 1 factorial scheme, with four replicates. Treatments comprised three sources (conventional uncoated urea, NBPT (N-(n-butyl) thiophosphoric triamide)-treated urea, and polymer-coated urea) and four rates (30, 60, 90 and 120 kg ha-1 ) of N, in addition to a control treatment (no fertilizer application). Microbial biomass C (MBC) and microbial biomass N (MBN), and soil respiration (C-CO 2 and qCO 2 ) were determined in upland rice rhizosphere in each crop season. Responses of soil microbial properties to N fertilization were dependent on the N rates, but no significant effect of the N sources was observed. All measured parameters, except MBC in the first season and C-CO 2 in the second season, were increased with increasing N rates. However, the application of N higher than 60 kg ha-1 suppressed soil microbial biomass, as well as soil respiration. Therefore, the lack of response by added urease inhibitors to the N sources indicate that optimizing N rates for upland rice production is a far more effective option for improving soil microbial community than using enhanced-efficiency N sources.