The role of enzymes in the angiogenic activity of Hancornia speciosa latex
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2022
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The Hancornia speciosa latex has shown angiogenic activity. Angiogenesis plays a major role in wound
healing, and materials that stimulate this process could be used to develop drugs. This study aimed to explain
the role of proteins in the H. speciosa serum fraction latex in angiogenesis. Hence, this material was treated
with proteinase K and the proteins were inactivated. After protein inactivation, angiogenic activity was
assessed with the chick chorioallantoic membrane assay. The result showed that the proteins in the serum
fraction are responsible for angiogenic activity. Then, the total protein content in the serum fraction and its
enzymatic activity were investigated. The low protein content observed in the H. speciosa serum fraction
latex suggests that this biomaterial could be used to develop new drugs with a hypoallergenic response.
Despite the low protein content, there was a significant enzymatic activity of at least three enzymes in the
serum fraction latex: β-1,3 glucanase, β-glucosidase, and proteases. These enzymes seem to influence the
healing process, assisting debridement, extracellular matrix remodeling, and collagen deposition, and
decreasing the chances of contamination by microorganisms. In conclusion, the enzymes in the H. speciosa
serum latex are associated with the angiogenic activity of this biomaterial and may be used to assist the
wound healing process.
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Chick chorioallantoic membrane, Proteinase K. wound healing, Enzymes
Citação
D'ABADIA, Patrícia Lima et al. The role of enzymes in the angiogenic activity of Hancornia speciosa latex. Bioscience Journal, Uberlândia, v. 38, e38086, 2022. DOI: https://doi.org/10.14393/BJ-v38n0a2022-61092. Disponível em: https://seer.ufu.br/index.php/biosciencejournal/article/view/61092. Acesso em: 4 maio 2023.