Monitoramento da carga viral de SARS-CoV-2 em águas residuais na cidade de Goiânia: epidemiologia baseada em esgoto e um sistema de alerta precoce para covid-19
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Data
2022-08-19
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Resumo
COVID-19 is currently the most critical disease in the world. Thus, several tools have been used in the
last two years to contain the spread of SARS-CoV-2. In this perspective, Wastewater-Based Epidemiology (WBE) provides valuable
data by including symptomatic, pre-symptomatic, and asymptomatic individuals and anticipating clinical cases. For 37 weeks, the
SARS-CoV-2 viral load was monitored in sanitary sewage samples from Goiânia-Brazil. Through pre-concentration, extraction, and
purification of viral RNA, followed by RT-qPCR, it was detected in 83.78% (31/37) of the weeks the presence of fragments of the
genetic material SARS-CoV-2 with viral loads ranging from 105 to 108
genome copies L-1. The viral load in the wastewater samples
was related to the clinical data of the municipality, demonstrating its ability to promote an Early Warning System (EWS). In early
2022, it was possible to predict the increase in clinical cases 3 weeks in advance and issue an alert note to the health authorities.
Prevalence estimates ranged from 0.003 to 0.5% and showed good agreement with observed values. Thus, monitoring effluents
become another essential tool that can help combat the pandemic’s advance, especially in countries with scarce resources.
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COVID-19, Wastewater surveillance, SARS-CoV-2, Sewage, Wastewater-based epidemiology, RT-qPCR
Citação
MENDES, Geovana de Melo et al. Monitoramento da carga viral de SARS-CoV-2 em águas residuais na cidade de Goiânia: epidemiologia baseada em esgoto e um sistema de alerta precoce para covid-19. Química Nova, São Paulo, v. 45, n. 10, p. 1197-1204, 2022. DOI: 10.21577/0100-4042.20170933. Disponível em: https://quimicanova.sbq.org.br/default.asp?ed=320. Acesso em: 13 dez. 2023.