Labeling mesenchymal cells with DMSA-coated gold and iron oxide nanoparticles: assessment of biocompatibility and potential applications
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2016
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Background: Nanoparticles’ unique features have been highly explored in cellular therapies. However, nanoparticles can be cytotoxic. The cytotoxicity can be overcome by coating the nanoparticles with an appropriated surface
modification. Nanoparticle coating influences biocompatibility between nanoparticles and cells and may affect some
cell properties. Here, we evaluated the biocompatibility of gold and maghemite nanoparticles functionalized with
2,3-dimercaptosuccinic acid (DMSA), Au-DMSA and γ-Fe2O3-DMSA respectively, with human mesenchymal stem cells.
Also, we tested these nanoparticles as tracers for mesenchymal stem cells in vivo tracking by computed tomography
and as agents for mesenchymal stem cells magnetic targeting.
Results: Significant cell death was not observed in MTT, Trypan Blue and light microscopy analyses. However, ultrastructural alterations as swollen and degenerated mitochondria, high amounts of myelin figures and structures similar
to apoptotic bodies were detected in some mesenchymal stem cells. Au-DMSA and γ-Fe2O3-DMSA labeling did not
affect mesenchymal stem cells adipogenesis and osteogenesis differentiation, proliferation rates or lymphocyte
suppression capability. The uptake measurements indicated that both inorganic nanoparticles were well uptaken by
mesenchymal stem cells. However, Au-DMSA could not be detected in microtomograph after being incorporated by
mesenchymal stem cells. γ-Fe2O3-DMSA labeled cells were magnetically responsive in vitro and after infused in vivo in
an experimental model of lung silicosis.
Conclusion: In terms of biocompatibility, the use of γ-Fe2O3-DMSA and Au-DMSA as tracers for mesenchymal stem
cells was assured. However, Au-DMSA shown to be not suitable for visualization and tracking of these cells in vivo by
standard computed microtomography. Otherwise, γ-Fe2O3-DMSA shows to be a promising agent for mesenchymal
stem cells magnetic targeting.
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Mesenchymal stem cells, Iron oxide nanoparticle, Gold nanoparticles, Biocompatibility, Computed microtomography, Magnetic targeting, DMSA-nanoparticles
Citação
SILVA, Luisa H. A. et al. Labeling mesenchymal cells with DMSA-coated gold and iron oxide nanoparticles: assessment of biocompatibility and potential applications. Journal of Nanobiotechnology, London, v. 14, e59, 2016. DOI: 10.1186/s12951-016-0213-x. Disponível em: https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-016-0213-x. Acesso em: 30 out. 2023.