Genetic heterogeneity of Escherichia coli strains isolated from raw milk, Minas Frescal cheese, and food handlers
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Data
2009
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Resumo
From February 2004 to March 2005, 140 samples of food handlers – hands and nostrils – (92), raw milk
(24), and minas frescal cheese (24) were analyzed for the presence of Escherichia coli in a dairy
processing plant of Goiás State. Forty-seven E. coli strains were obtained and compared by DNA
macrorestriction patterns obtained from pulsed-field gel electrophoresis following XbaI restriction in
order to investigate the possible sources of cheese contaminations. Based on PFGE genotyping, one
strain isolated from food the hands of a handler and five strains isolated from raw milk were identical or
closely related to six strains from cheese suggesting, in these cases, the probable source of E. coli
contamination in cheeses. No strain isolated from the nostrils was related to those found in cheeses or
milk strains. The results showed high diversity among the strains, demonstrating a lack of predominance
of an endemic clone in the dairy plant. This paper highlights the usefulness of PFGE as an
epidemiological tool for determining the source of E. coli contamination in the food industry.
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Escherichia coli, Pulsotypes, Minas frescal cheese, Raw milk, Food handlers, Escherichia coli, PFGE, Leite cru, Queijo Minas Frescal, Manipuladores
Citação
CAMPOS, M. R. H.; ANDRÉ, M. C. D. P. B.; BORGES, L. J.; KIPNIS, A.; PIMENTA, F. C.; SERAFINI, A. B. Genetic heterogeneity of Escherichia coli strains isolated from raw milk, Minas Frescal cheese, and food handlers. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 61, n. 5, p. 1203-1209, 2009.