Validação de técnica analítica em cromatografia em camada delgada comparativa para identificação de fármacos anorexígenos sintéticos em produtos fitoterápicos
Data
2004-12
Título da Revista
ISSN da Revista
Título de Volume
Editor
Marize Campos Valadares
Resumo
There are reports of synthetic substances not mentioned in the composition of slimming
phytotherapics freely marketed being they responsible for the apparently miraculous effects of these formulations.
These substances act in the Central Nervous System (CNS) with sale and use restrictions. Due the need to identify
those adulterants, a comparative thin layer chromatography technique (TLC) was validated for drugs (1mg/mL in
methanol solution) used in the treatment of obesity (femproporex, mazindol, diethylpropion, fenfluramine,
hydrochlorotiazide, furosemide, fluoxetine, benzocaine, diazepam, midazolam, flurazepam, bromazepam,
nitrazepam, clordiazepoxide, caffeine, phenolphthalein). Analytical interference with 20 mg of standard phytodrugs
(Cynara scolynmus, Aloe vera, Peumus boldus, Baccaharis sp, Rhamnus purshiana, Centella asiatica, Garcinia sp,
Chorella pyrenoidosa, Maytenus ilicifolia, Spirulina maxima, Fucus sp., Paullinia cupana, Pfaffia paniculata,
Amorphophallus konjac, Solanum paniculatum, Passiflora alata Cassia angustifolia) extracted with 5 mL of methanol was tested. Analytical technique of TLC was developed in silica gel G60’s plate, 0.5 mm, 20x20 cm, with
mobile phase and sequence of detection reagents for substances of acid/neutral and basic characters. The Rf’s
values and developed spot colors were considered. The procedure for the "natural products” was performed, whose
results were compared to the patterns, with repetitions for the suspicious products. It was concluded that the
technique is selective, fast and simple and have low cost. It could be developed at any analytic laboratory for the
identification of the synthetic anorexic drugs in phytomedicine composition.
Descrição
Palavras-chave
CCDC, Cromatografia, Anorexigeno, Fitoterápico, Cromatografia, Padronização, TLC, Chromatography, Anoretic drug, Phytotherapics, Validation
Citação
AZEREDO, Flaubertt Santana de; GUIMARÃES, Renato Inácio; PAULA, José Realino de; CUNHA, Luiz Carlos da. Validação de técnica analítica em cromatografia em camada delgada comparativa para identificação de fármacos anorexígenos sintéticos em produtos fitoterápicos. Revista Eletrônica de Farmácia, Goiânia, v. 1, n. 1, p. 17-24, jan./dez. 2004. Disponível em: <https://www.revistas.ufg.br/REF/article/view/1751/1848>.