Effects of nerolidol and limonene on stratum corneum membranes: a probe EPR and fluorescence spectroscopy study
Nenhuma Miniatura disponível
Data
2017
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
The sesquiterpene nerolidol and the monoterpene limonene are potent skin-permeation enhancers that have also
been shown to have antitumor, antibacterial, antifungal and antiparasitic activities. Because terpenes are
membrane-active compounds, we used electron paramagnetic resonance (EPR) spectroscopy of three membrane
spin labels combined with the fluorescence spectroscopy of three lipid probes to study the interactions of these
terpenes with stratum corneum (SC) intercellular membranes. An experimental apparatus was developed to
assess the lipid fluidity of hydrated SC membranes via the fluorescence anisotropy of extrinsic membrane probes.
Both EPR and fluorescence probes indicated that the intercellular membranes of neonatal SC rats undergo a main
phase transition at approximately 50 °C. Taken together, the results indicated that treatment with 1% nerolidol
(v/v) caused large fluidity increases in the more ordered phases of SC membranes and that these effects gra dually decreased with increasing temperature. Additionally, compared with (+)-limonene, nerolidol was better
able to change the SC membrane dynamics. EPR and fluorescence data suggest that these terpenes act as spacers
in lipid packaging and create increased lipid disorder in the more ordered regions and phases of SC membranes,
notably leading to a population of probes with less restricted motion.
Descrição
Palavras-chave
Stratum corneum, Fluorescence probe, Nerolidol, Spin label
Citação
MENDANHA, Sebastião Antonio; MARQUEZIN, Cássia Alessandra; ITO, Amando Siuiti; ALONSO, Antonio. Effects of nerolidol and limonene on stratum corneum membranes: a probe EPR and fluorescence spectroscopy study. International Journal of Pharmaceutics, Amstedam, v. 532, p. 547-554, 2017. DOI: 10.1016/j.ijpharm.2017.09.046. Disponível em: https://pubmed.ncbi.nlm.nih.gov/28935253/. Acesso em: 26 maio 2023.