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Item Caracterização de extratos de própolis e efeito sobre Campylobacter termotolerantes de origem avícola(Universidade Federal de Goiás, 2020-02-29) Calazans, Luíza Toffano Seidel; Andrade, Maria Auxiliadora; http://lattes.cnpq.br/9441751521255467; Andrade, Bruna Aparecida Souza; http://lattes.cnpq.br/4021221951532780; Rezende, Cíntia Silva Minafra e; http://lattes.cnpq.br/5841210447886226; Rezende, Cíntia Silva Minafra e; Teixeira, Weslen Fabrício Pires; Tenório, Clarice Gebara Muraro Serrate CordeiroThermotolerants Campylobacter are related to a group of pathogens that are carried by food and are currently considered as the main ones of global occurrence and with high frequency of resistance to antimicrobials. From the above, the present study aimed to identify biocomposites present in four extracts of green and red propolis and to evaluate alternatives regarding the antimicrobial potential. Extracts from Brazil, obtained by conventional ethanol extraction and extraction assisted by ultrasound, were evaluated. Four cultures of collections and twenty-five isolates from three species (Campylobacter jejuni, Campylobacter coli and Campylobacter lari) comprised the study. The technique used was microdilution in broth in a 96-well plate, which allowed the analysis of eleven concentrations of the propolis extracts (0.01 to 100 mg / mL-1). Variability was observed for antimicrobial activity. Greater microbial viability and motility were identified by optical microscopy at the highest concentrations of propolis tested. In addition, regarding the effect on bacterial viability of red propolis in relation to green propolis, the red one showed more efficient. There was no difference in extraction methods. It is concluded that the propolis extracts had an unsatisfactory antimicrobial effect, as well as a cellular protective effect for high concentrations in this study, regardless of the extraction method, which may have a desirable connotation for laboratory diagnosis.Item Fatores de risco para infecção pelos vírus da diarreia viral e rinotraqueíte infecciosa bovina no Sítio Histórico e Patrimônio Cultural Kalunga(Universidade Federal de Goiás, 2016-03-04) Dias, Juliana Moraes; Arnhold, Emmanuel; http://lattes.cnpq.br/7156945506134934; Juliano, Raquel Soares; http://lattes.cnpq.br/4884381347303453; Fioravanti, Maria Clorinda Soares; http://lattes.cnpq.br/8772502020076257; Fioravanti, Maria Clorinda Soares; Pimentel, Concepta Margaret McManus; Moura, Maria Ivete deThe research consisted of evaluating the frequency of seropositive animals for viral diarrhea and infectious bovine rhinotracheitis and determining correlations with environmental, social and health characteristics of the region. The study was carried out in herds of the Kalunga’s Historical and Cultural Heritage Site, located in the north of the state of Goiás, it is an area of Cerrado with native pastures and few formed areas. Blood samples were collected from 2,612 cattle of different breeds and age groups, male and female, corresponding to 79 predominantly beef cattle. During sample collection, an epidemiological questionnaire was applied to investigate factors that could be associated with infections. The samples were submitted by the ELISA method, to detect antibodies against BVD and IBR. The average frequency of seropositive herds was 88.6% for BVD and 97% for IBR, the individual positive serology corresponded to 17.8% for BVD and 45.5% for IBR. There was a significant difference (p <0.05) between the regions and age group for both diseases. The environmental variables associated with the frequency of BVD were: level of precipitation and maximum temperature of the hottest month of the year, and for IBR: INDV and temperature seasonality. Contact with other species of animals, reproductive problems, presence of wetland, use of cattle as the main source of income, educational level of the producer and marital status were correlated risk factors for the occurrence of BVD and IBR.Item Detecção dos genes spvC e prot6E e avaliação da infecciosidade de Salmonella sp. em poedeiras comerciais(Universidade Federal de Goiás, 2019-03-06) Figueira, Samantha Verdi; Rezende, Cíntia Silva Minafra e; http://lattes.cnpq.br/5841210447886226; Moraes, Dunya Mara Cardoso; http://lattes.cnpq.br/9632713129112672; Andrade, Maria Auxiliadora; http://lattes.cnpq.br/9441751521255467; Andrade, Maria Auxiliadora; Faria, Adriana Marques; Rocha, Fernanda Rodrigues Taveira; Stringhini, José Henrique; Teixeira, Weslen Fabrício PiresThe pathogenesis of Salmonella includes different factors, such as plasmidial genes, which because they are mobile genetic mechanisms can increase the bacterial genetic diversity contributing to the modification of virulence and adaptation to the hosts. The present work was developed to detect the presence of the virulence genes spvC and prot6E in different serovars of Salmonella enteric present in the metropolitan region of Goiânia and to verify the ability of these serovars to express their pathogenicity in embryonated eggs, neonates and laying hens and to cause contamination in eggs in experimental models. In Experiment 1, isolates of Salmonella Enteritidis, Gallinarum, Heidelberg, Infantis, Schwarzengrund and Typhimurium were obtained from organs of sick bird, eggs and environment of poultry chain and were investigated the presence of spvC and prot6E genes by real-time PCR. Of the 47 isolates, 15 (31.9%) were positive for the spvC gene and five (10.6%) for the prot6E gene. It is concluded that Salmonella Enteritidis, Typhimurium and Gallinarum, isolated from the bird and eggs contain the spvC genes. The prot6E gene was detected in the serovars Salmonella Enteritidis, Heidelberg and Typhimurium from egg samples. The serovars of Salmonella Heidelberg, Infantis, Schwarzengrund and Typhimurium from environmental samples do not have the plasmid genes spvC and prot6E. In experiment 2, two isolates of Salmonella Gallinarum, one positive and one negative for the spvC gene, were inoculated into embryos and neonates of laying birds to investigate whether the presence of the spvC gene in the Gallinarum serovar is able to determine embryonic mortality, affect the quality of the neonate and chick, production parameters and cause systemic disease in young birds. It was observed high mortality of the groups inoculated with Salmonella Gallinarum in alantoid route with one and 14 days of incubation, independent of the presence of the spvC gene. No embryonic mortality, changes in neonatal weight / egg weight and changes in neonatal quality were observed for birds inoculated through a 19-day in air chamber. Salmonella Gallinarum without and with the spvC gene was recovered from the heart, spleen, liver and ceca of birds inoculated in the air chamber, whereas for the oral inoculated group, the pathogen without the gene was recovered only in the ceca and the pathogen with the gene was recovered from spleen and cecum. Salmonella inoculation led to decreased weight gain and altered biometry of the heart and intestine. It is concluded that Salmonella Gallinarum is capable of causing embryonic mortality in embryos inoculated with one and 14 days in the allantoic cavity. The presence of the spvC gene in Salmonella Gallinarum does not affect embryo mortality, incubation parameters and systemic dissemination for birds inoculated in the air chamber. For birds inoculated by the oral route, the presence of the spvC gene determines enteric and systemic infection, whereas the isolate without the gene remains restricted to the cecum. In Experiment 3, the Salmonella Heidelberg isolate positive for the prot6E gene in experiment 1 was inoculated in laying hens in the oral, intravaginal and intravenous routes in order to investigate their ability to cause clinical disease, egg and gastrointestinal tract contamination. The pathogen was isolated in the excreta, only 12 hours after inoculation in 33% of the birds inoculated from oral route and 66% of the birds inoculated intravaginal route. In the eggs, the pathogen was isolated at 24 and 48 hours, seven and 15 days. It is concluded that the presence of the prot6E gene in Salmonella Heidelberg is not sufficient to cause clinical salmonellosis in laying hens, but it determines contamination of the eggs and the gastrointestinal tract of chickens, regardless of the route of inoculation, oral, intravaginal and intravenous.Item Efeito da fotobiomodulação e de células-tronco derivadas do tecido adiposo na reparação de feridas por queimadura térmica em ratos(Universidade Federal de Goiás, 2020-07-31) Ribeiro, Maisa; Moraes, Julia de Miranda; http://lattes.cnpq.br/7729197454739642; Vulcani, Valcinir Aloisio Scalla; http://lattes.cnpq.br/0967468447941793; Vulcani, Valcinir Aloisio Scalla; Prado, Rodrigo Paschoal; Araújo, Gustavo Henrique Marques; Saturnino, Klaus Casaro; Rita, Ricardo de Mattos SantaIntroduction: Injuries caused by full-thickness burns are considered a severe form of trauma with a high worldwide morbidity and mortality rate capable of promoting destruction of skin tissue and morphologically compromising the organism. The use of stem cells derived from adipose tissue (ADSC) and low-level laser (LLL) are promising therapeutic alternatives explored by regenerative medicine. The aim of this study was to establish an effective isolation model for more homogeneous ADSC cells; to evaluate the effects of ADSC and LBP on the skin healing process and to investigate the association of techniques (LBP and ADSC) in the treatment for full-thickness burn in an experimental rat model. Material and methods: The obtaining of the ADSC was carried out by means of imonumagnetic isolation by CD49d protein and conventional culture. A total of 150 Wistar rats were divided into a control group and groups treated with ADSC CD49d positive, ADSC CD49d negative, conventionally isolated stem cells (CULT), LLL, ADSC+LLL, ADSC-LLL. Scald burns were induced on the back of each animal, with subsequent surgical debridement. LLL with a wavelength of 660 nm, 30 mW of power at five points of the wound was used, under total fluence of 450 Joules / cm2 and total application of 5 Joules. Macroscopic and histopathological analyzes were performed according to biopsies collected on days 5, 14 and 21 post-injury, processed and stained using the techniques of Hematoxylin and Eosin (HE) and Gomori's Trichrome (GT). Statistical analyzes were performed using Tukey's test and analysis of variance (ANOVA), Kruskal-Wallis test (non-parametric) and T-Paired test. For all analyzes, a significance level of 5% (p <0.05) was used. Results: The results showed a statistical difference in morphometry between the groups with the best degree of reepithelization and reduction in the lesion area presented by the ADSC+ and CULT treatments at 21 days of biopsy. Histopathological analysis showed that ADSC+, LLL and ADSC+LLL treatments significantly influenced the healing process, showing improvement in the increase of mononuclear inflammatory infiltrates, greater angiogenesis, decreased edema, increased granulation tissue, in the number of fibroblasts at the beginning of tissue repair, in addition to promoting re-epithelialization in a more organized manner with greater deposition of collagen and formation of epithelial attachments at 21 days after the injury. Conclusion: low-power laser photobiomodulation of ADSC cells enhanced the repair process in full-thickness burn wounds, demonstrating a significant interaction for the remodeling of the extracellular matrix and skin tissue. Additional studies are needed to measure the effects of LBP and the activity of ADSCs on skin tissue repair.Item Função sistólica do ventrículo esquerdo em cães com doença mixomatosa valvar mitral(Universidade Federal de Goiás, 2019-12-27) Sousa, Paulo Roberto de; Damasceno, Adilson Donizeti; http://lattes.cnpq.br/3900110295277130; Torres, Bruno Benetti Junta; http://lattes.cnpq.br/7060242425070126; Carvalho, Rosângela de Oliveira Alves; http://lattes.cnpq.br/5593911382353878; Carvalho, Rosângela de Oliveira Alves; Sousa, Marlos Gonçalves; Damasceno, Adilson DonizetiMyxomatous mitral valve disease (MMVD), or mitral valve endocardiosis, is the most prevalent acquired heart disease in dogs, being the main cause of heart failure in small dogs. The echocardiographic examination, which allows cardiac morphological and functional evaluation, is considered the “gold standard” test for diagnosing and staging MMVD in dogs. Information obtained by echocardiography on ventricular function also determines the prognosis in dogs with MMVD, since systolic dysfunction is a risk factor in these patients and contributes to the development of heart failure. However, the assessment of ventricular systolic function using conventional echocardiographic indexes is limited, since it is directly influenced by preload and afterload factors. The evaluation of cardiac mechanics by strain (ST) and strain rate (STR), using two-dimensional speckle tracking (2D-STE), allows, in humans, an earlier diagnosis of ventricular dysfunction. In addition, the mitral ring tissue displacement (TMAD) is an index of longitudinal systolic function that has a good correlation with conventional echocardiographic indices and low intra and interobserver variability. Thus, the objective of this study was to verify whether the myocardial deformity indexes (ST and STR) and the TMAD are more accurate and precocious than the conventional echocardiographic indexes in the assessment of left ventricular systolic function in dogs with MMVD, measuring the variables in the day of diagnosis (T0) and after 30 days (T30). For this purpose, 29 dogs were evaluated, categorized according to the ACVIM consensus, eight of which were in stage B1, 12 in stage B2 and nine in stage C. Considering the conventional variables used in this study, the presence of systolic dysfunction was not observed in dogs with MMVD, however changes after the start of pharmacological treatment were observed in the variables FET% and MAM. From the 2D-STE evaluation, similar to the conventional evaluation, systolic dysfunction cannot be determined in dogs with MMVD. However, the longitudinal ST variable indexed by weight showed a significant increase between the assessment times in group C, being still significantly higher in C than in B2, at administration, and higher in C than in the other groups after treatment. For the variable longitudinal STR it decreased in T30 in B1 and increased in C. When comparing the groups, in T30, group C showed higher values than in B1 and B2. Still, the variables longitudinal ST and TMAD showed a significant negative correlation when indexed to weight. In conclusion, the assessment of myocardial deformity using 2D-STE assessment did not demonstrate superiority compared to conventional indices for the diagnosis of systolic dysfunction in dogs with MMVD in its different stages.Item Ultrassonografia da articulação femorotibiopatelar de ovinos com sinovite experimental(Universidade Federal de Goiás, 2019-01-17) Souza, Michel Felipe Soares; Borges, Naida Cristina; http://lattes.cnpq.br/9181279951885005; Silva, Marco Augusto Machado; http://lattes.cnpq.br/2134206254307043; Silva, Marco Augusto Machado; Borges, Naida Cristina; Silva, Marcelo Seixo de Brito e; Cardoso, Júlio Roquete; Souza, Luiz Augusto deThe ovine stifle joint is complex and multiple bone and soft tissue elements comprise its anatomy. It is used as an experimental model for human stifle joint disease studies. Articular ultrasonography is an important diagnostic technique for articular diseases. However, there is a gap in the literature regarding ultrasound assessment of the ovine stifle joint as an experimental model. Thus, the purpose of this study was to describe sonographic changes of the ovine stifle joint undergone experimentally induced synovitis. Twelve ovine received intra-articular infusion of E. coli lipopolysaccharide in the right stifle joint to induce synovitis. Injected stifles were assessed by ultrasound at baseline and 12, 24, 48, 72 and 120 hours after intra-articular injection of lipopolysaccharide. Nine ultrasound positions were used to assess the stifle joint: suprapatelar area; lateral area of the femorotibial compartment; infrapatelar space; medial area of the femorotibial compartment; lateral femoropatelar compartment; medial femoropatelar compartment; craniolateral area; sheath of the origin of the extensor muscle tendons; and medial supracondilar recess. Stifle joint was evaluated for volume changes in the synovial fluid, thickness of the synovial membrane and cellularity. Intra-articular lipopolysaccharide injection yielded echographic changes in seven out of nine scanning positions at the ovine stifle joint. Changes were observed when the medial femorotibial compartment was evaluated in 11 animals (91.7%), in the craniolateral region, in 10 animals (83,34%), in the tendinous recess, in 11 animals (91.7%), and in the infrapatellar space, lateral and medial aspects of the femoropatellar compartments and medial supracondilar recess in all animals (100%). It was not observed alterations within the suprapatellar region and the lateral femorotibial compartment. Stifle joint ultrasound assessment was efficient for detection of intra-articular changes following infiltration with lipopolysaccharide in the ovine experimental model. The most observed signs were increase of the synovial fluid volume and thickening of the synovial membrane, 12, 24 and 48 hours after infiltration.Item Comparação dos métodos de controles estratégico e seletivo na carga parasitária de Rhipicephalus microplus em rebanhos bovinos leiteiros(Universidade Federal de Goiás, 2020-03-02) Vieira, Luciano da Silva; Cotta, Viviane Zeringóta Rodrigues; http://lattes.cnpq.br/4269635925947346; Monteiro, Caio Márcio de Oliveira; http://lattes.cnpq.br/2496419306394657; Borges, Lígia Miranda Ferreira; http://lattes.cnpq.br/2591106734434348; Borges, Lígia Miranda Ferreira; Cotta, Viviane Zeringóta Rodrigues; Monteiro, Caio Márcio de Oliveira; Louly, Carla Cristina Braz; Lopes, Wélber Daniel ZanettiRhipicephalus microplus is one of the main ectoparasites of economic importance for cattle in tropical and subtropical regions, causing drop in milk production and transmitting pathogenic agents such as Anaplasma and Babesia. Therefore, constant studies on treatment schemes for parasite control are necessary to minimize the damage and losses that follow infestations by this arthropod. In this way, the present study aimed to compare two methods for R. microplus control, the strategic method and the selective method, in dairy cattle herds, aiming to provide support to the technical guidelines on the most efficient control method of the parasitic load. In the strategic method, seven treatments with spray acaricides were carried out every 21 days, starting in November. In the selective method, treatments with acaricides were performed only on animals that had an infestation equal to or greater than 40 engorged females. Only 5% of animals submitted to the selective method received treatment in November. The average of tick counts was lower in the strategic method compared to the selective method, in December. The maximum peak of infestation occurred in January, in both control methods, with no significant difference, persisting until February. No animal of the strategic method had an infestation greater than 40 engorged females in March. However, the parasitic load in both control methods was similar in the second evaluation in March. The averages of counts in the strategic method were lower in the months of April (10.9) and May (16.9) compared to the averages observed in the selective method (56.3 and 48.4). Yet, it was necessary to carry out tactical treatments in the strategic method, after the treatment scheme, in up to 16.7% of the animals in April and up to 50.0% in May, while in the selective method, the treatments varied from 42.8 to 88.8% in April and in May it varied from 28.6 to 75.0%. The total count of R. microplus was 5,036 engorged females in the strategic method, while in the selective method there were 13,829 engorged females in the study period. Therefore, it is concluded that the treatment scheme for control of R. microplus significantly interfered in the parasitic load of dairy cows, favouring the maintenance of acceptable levels of parasitism when the strategic method of tick control was implemented compared to the adoption of the selective method.Item Efeitos dos extratos de folhas de campomanesia adamantium e hymenaea martiana hayne sobre células de osteossarcoma canino e células endoteliais humanas(Universidade Federal de Goiás, 2020-02-28) Vieira, Vanessa de Souza; Cruz, Vanessa de Sousa; http://lattes.cnpq.br/1222753642148629; Araújo, Eugênio Gonçalves de; http://lattes.cnpq.br/391977757005992; Araújo, Eugênio Gonçalves; Pfrimer, Gabriel de Abreu; Pereira, Kleber Fernando; Arnhold, Emmanuel; Oliveira, Gerlon de Almeida Ribeiro deOsteosarcoma (OSA) is a very aggressive tumor in dogs, with low survival rate and ineffective treatment. In the search for alternative sources of therapy, the Brazilian biome becomes a scientific hope, presenting a diversity of medicinal plants of popular knowledge and use. However, they can have controversial biological functions. The aim of this study was to verify the bioactivity of the leaves of Campomania adamantium and Hymenaea martiana Hayne on canine osteosarcoma (OC) cells from cell cultures and human umbilical vein endothelial cells (HUVEC). As cells were cultured and subjected to treatment with C. adamantium (1 μg / mL, 10 μg / mL, 100 μg / mL, 1000 μg / mL) and H. martiana Hayne (10μL / mL, 100μL / mL, 1000μL / mL , 2000μL / mL and 5000μL / mL), in the 24h, 48h and 72h exposure periods in normoxia and 24h in cells exposed to oxidative stress, induced by hydrogen peroxide. The results were analyzed by analyzing cell viability and cytotoxicity using the tetrazolium reduction method (MTT). This study brings unprecedented results in relation to extractive effects, as it was possible to demonstrate that they have little cytotoxic action. In addition, it was observed that the higher the dosage and the longer the exposure time, the greater the proliferative activity, with increased cell viability in the 72-hour group. In endothelial cells, similarly, there was no cytotoxic activity, in addition to the occurrence of increased cell viability, even after submission to oxidative stress. C. adamantium leaf extract increased viability and did not show cytotoxic action in canine osteosarcoma cells and endothelial cells under oxidative stress. The crude ethanolic extract of the leaves of Hymenaea martiana Hayne, increased the cell viability of canine osteosarcoma cells and human umbilical vein endothelial cells, submitted to oxidative stress.