Efeito da fotobiomodulação e de células-tronco derivadas do tecido adiposo na reparação de feridas por queimadura térmica em ratos
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2020-07-31
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Universidade Federal de Goiás
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Introduction: Injuries caused by full-thickness burns are considered a severe form of trauma with a high worldwide morbidity and mortality rate capable of promoting destruction of skin tissue and morphologically compromising the organism. The use of stem cells derived from adipose tissue (ADSC) and low-level laser (LLL) are promising therapeutic alternatives explored by regenerative medicine. The aim of this study was to establish an effective isolation model for more homogeneous ADSC cells; to evaluate the effects of ADSC and LBP on the skin healing process and to investigate the association of techniques (LBP and ADSC) in the treatment for full-thickness burn in an experimental rat model. Material and methods: The obtaining of the ADSC was carried out by means of imonumagnetic isolation by CD49d protein and conventional culture. A total of 150 Wistar rats were divided into a control group and groups treated with ADSC CD49d positive, ADSC CD49d negative, conventionally isolated stem cells (CULT), LLL, ADSC+LLL, ADSC-LLL. Scald burns were induced on the back of each animal, with subsequent surgical debridement. LLL with a wavelength of 660 nm, 30 mW of power at five points of the wound was used, under total fluence of 450 Joules / cm2 and total application of 5 Joules. Macroscopic and histopathological analyzes were performed according to biopsies collected on days 5, 14 and 21 post-injury, processed and stained using the techniques of Hematoxylin and Eosin (HE) and Gomori's Trichrome (GT). Statistical analyzes were performed using Tukey's test and analysis of variance (ANOVA), Kruskal-Wallis test (non-parametric) and T-Paired test. For all analyzes, a significance level of 5% (p <0.05) was used. Results: The results showed a statistical difference in morphometry between the groups with the best degree of reepithelization and reduction in the lesion area presented by the ADSC+ and CULT treatments at 21 days of biopsy. Histopathological analysis showed that ADSC+, LLL and ADSC+LLL treatments significantly influenced the healing process, showing improvement in the increase of mononuclear inflammatory infiltrates, greater angiogenesis, decreased edema, increased granulation tissue, in the number of fibroblasts at the beginning of tissue repair, in addition to promoting re-epithelialization in a more organized manner with greater deposition of collagen and formation of epithelial attachments at 21 days after the injury. Conclusion: low-power laser photobiomodulation of ADSC cells enhanced the repair process in full-thickness burn wounds, demonstrating a significant interaction for the remodeling of the extracellular matrix and skin tissue. Additional studies are needed to measure the effects of LBP and the activity of ADSCs on skin tissue repair.
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RIBEIRO, M. Efeito da fotobiomodulação e de células-tronco derivadas do tecido adiposo na reparação de feridas por queimadura térmica em ratos. 2020. 148 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2020.