Doutorado em Ciência Animal (EVZ)
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Item Avaliação de novas opções terapêuticas para infecção causada pela Rickettsia rickettsii(Universidade Federal de Goiás, 2025-03-17) Paula, Warley Vieira de Freitas; Martins, Danieli Brolo; http://lattes.cnpq.br/2159963349521553; Labruna, Marcelo Bahia; http://lattes.cnpq.br/9096422507543208; Krawczak, Felipe da Silva; http://lattes.cnpq.br/6875905418744479; ; Krawczak, Felipe da Silva; http://lattes.cnpq.br/6875905418744479; Angerami, Rodrigo Nogueira; http://lattes.cnpq.br/7698815158936976; Santos, Adriano Pinter dos; http://lattes.cnpq.br/8887336916442663; Muñoz-Leal, Sebastián; http://lattes.cnpq.br/9816006648370440; Bermúdez, SergioEmbargado.Item Eficácia de fungos e nematoides entomopatogênicos testados isoladamente ou em combinação para controle de Rhipicephalus microplus: do laboratório para o campo(Universidade Federal de Goiás, 2021-12-20) Gomes, Marcos Daniel Filgueiras; Monteiro, Caio Márcio de Oliveira; http://lattes.cnpq.br/2496419306394657; Soares, Filippe Elias de Freitas; http://lattes.cnpq.br/1857312169349768; Fernandes, Éverton Kort Kamp; http://lattes.cnpq.br/2135541732341157; Fernandes, Éverton Kort Kamp; http://lattes.cnpq.br/2135541732341157; Carvalho , Vanessa Andaló Mendes de; http://lattes.cnpq.br/8132224406035792; Camargo, Mariana Guedes; http://lattes.cnpq.br/1797246939020030; Bernardo, Cíntia das Chagas; http://lattes.cnpq.br/7827463777771968; Borges, Lígia Miranda Ferreira; http://lattes.cnpq.br/2591106734434348Entomopathogenic fungi and nematodes are biocontrol agents widely investigated for the biological control of several pests, including ticks. This study aimed to determine the efficacy of fungi and nematodes, combined or not combined, against Rhipicephalus microplus engorged females under laboratory and field conditions. Bioassays under controlled conditions were conducted to evaluate the pathogenicity of the nematode Heterorhabditis bacteriophora HP88 against R. microplus females with different weights (150, 200, 250, 300, and 350 mg), and against females from eight different populations. In addition, a study under field conditions were conducted to evaluate the efficacy of H. bacteriophora HP88 formulated on Tenebrio molitor cadavers for controlling non-parasitic R. microplus on infested pastures. Likewise, a study was sought to determine, under controlled conditions, the synergistic combination between Metarhizium spp. and H. bacteriophora HP88 against R. microplus engorged females, as well as to determine a granular formulation containing M. robertsii IP 146 microsclerotia with potential to produce conidia when incubated onto different substrates. Lastly, a study was conducted to determine, under field conditions, the efficacy of combined granular formulation and H. bacteriophora HP88 infective juveniles (third stage) for controlling non-parasitic R. microplus population on infested pastures during different seasons of the year (wet or dry). Nematode pathogenicity to R. microplus females did not show variation of the percent control (98.4% to 97.5%) on the groups with different weights. There were also no differences on susceptibility of females from different populations (percent control between 89% and 99%) to HP88. In the field test, non-parasitic R. microplus population was reduced by 73.1% in the plots treated with H. bacteriophora HP88. Combined treatments with Metarhizium spp. and H. bacteriophora HP88 reduced biological parameters of engorged females, with additive effect detected. However, treatmented with M. robertsii IP 146 combined with H. bacteriophora HP88 sub-doses demonstrated synergism. The field test conducted in the wet season reduced the non-parasitic R. microplus population on infested pastures, with efficacy of 54.09% (fungus only), 38.11% (nematode only), and 46.72% (fungi and nematodes combined). In the dry period, only the fungal treatment reduced R. microplus population, with efficacy of 26.27%. We concluded that M. robertsii IP 146 and H. bacteriophora HP88 are promising agents for biological control of the non-parasitic phase of R. microplus, although the treatment efficacy had varied according to the wet or dry seasons.Item Ultrassonografia em modo B e elastografia do grupo muscular quadríceps femoral e da articulação femorotibiopatelar de cães hígidos e com ruptura do ligamento cruzado cranial(Universidade Federal de Goiás, 2024-12-20) Silva, Wanessa Patrícia Rodrigues da; Cardoso, Júlio Roquete; Oliveira, Raquel de Souza Lemos de; Borges, Naida Cristina; http://lattes.cnpq.br/9181279951885005; Borges, Naida Cristina; Lima, Aline Maria Vasconcelos; Pfrimer, Gabriel de Abreu; Neves, Carla Amorim; Martins, Apóstolo FerreiraEmbargado.Item Doença respiratória em bovinos criados extensivamente(Universidade Federal de Goiás, 2025-02-24) Passos, Pedro Henrique Miranda; Borges, Naida Cristina; http://lattes.cnpq.br/9181279951885005; Martins, Danieli Brolo; http://lattes.cnpq.br/2159963349521553; Cunha, Paulo Henrique Jorge da; Cunha, Paulo Henrique Jorge da; Queiroz, Paulo José Bastos; Cerqueira, Aline BarichelloBovine respiratory disease (BRD) is a syndrome that affects cattle and can have a variety of etiologies. The present study presents a review of the literature on this disease, focusing on clinics, laboratories and ultrasounds, as tools to aid the diagnosis of BRD and viable to be carried out in the field. Furthermore, the present study objective to describes clinical, hematological, ultrasonographic, microbiological and histopathological findings of an outbreak of BRD in extensively reared elite Nellore calves. In these animals, based on clinical results and plasma fibrinogen values, eight animals (21.6%) from a batch of 37 calves were selected to undergo thoracic ultrasound. Of these cattle, seven (87.5%) presented images compatible with DRB. The ultrasound findings of these animals showed a significant difference (p = 0.0041) in animals with hyperfibrinogenemia (828.6 ± 372.9 mg/dL) when compared to animals with normal fibrinogen values (513.3 ± 208.0 mg/dL). The results reinforce the importance of combining different diagnostic techniques in the detection of DRB, in addition to indicating the potential for using plasma fibrinogen as a biological marker for the selection of animals in which thoracic ultrasound is indicated.Item Efeito Antitumoral Do Extrato Metanólico Do Látex De Synadenium Grantii Hook f. (Euphorbiaceae) Em Tumor De Ehrlich Sólido(Universidade Federal de Goiás, 2025-02-20) Nascente, Eduardo de Paula; Alves, Carlos Eduardo Fonseca; http://lattes.cnpq.br/7622517973469222; Paula, Joelma Abadia Marciano de; http://lattes.cnpq.br/0118857572417556; Moura, Veridiana Maria Brianezi Dignani de; http://lattes.cnpq.br/8773201078957745; Moura, Veridiana Maria Brianezi Dignani de; Santos, Adriana da Silva; Porto, Regiani Nascimento Gagn; Ronchi, Alessandra Aparecida Medeiros; Sant’Ana, Fabiano José de FerreiraMammary tumors are frequent in both women and female dogs, representing the most common neoplasms in these species. Among the similarities between mammary tumors in the two species are spontaneous development, tumor progression, biological behavior, and the expression of certain receptors. Thus, the female dog serves as a natural model for this disease. Furthermore, some tumors pose therapeutic challenges, necessitating new therapies when the goal is to improve patient prognosis. In this context, various chemotherapeutic drugs originate from botanical compounds, and the Brazilian Cerrado presents a diversity of plants with potential for the identification of new drugs. Synadenium grantii HOOK F. (Euphorbiaceae), a botanical species used in ethnomedicine in the Central-western and southern regions of Brazil, is empirically used in treating a range of diseases, including cancer. Therefore, this study aimed to evaluate the antiproliferative potential of the methanolic extract of S. grantii latex, administered intraperitoneally, in mice bearing Ehrlich solid tumors (EST), as well as its effects on the tumor microenvironment and the performance of treated animals. Additionally, phorbol esters in the botanical specimen were investigated. Initially, samples of the latex from a specimen of Synadenium grantii were collected in the municipality of Nerópolis, Goiás, Brazil. The latex was diluted in bidistilled water, subjected to lyophilization, and used to prepare the methanolic extract. Subsequently, experimental induction of TES was performed on the dorsum of adult female mice, which were treated with four weekly intraperitoneal applications of methanolic latex extract from S. grantii (MLE) at doses of 5, 15, and 25 mg/kg. Three control groups were utilized, including vehicle control, negative control, and carboplatin. After 7, 14, 21, and 35 days, animals were euthanized, and tumor fragments were collected for histological and immunohistochemical (anti-Ki67 and anti-CD31) evaluations. Phytochemical analysis was performed on the MEL sample using high-performance liquid chromatography coupled with high-resolution mass spectrometry (HPLC-HRMS). Furthermore, the survival rate and weight gain of the animals were determined. The phytochemical analysis identified a phorbol compound (phorbol-12,13,20-triacetate), and the 25 mg/kg dose of S. grantii MEL resulted in a reduced survival rate and weight gain in the animals. Conversely, the 15 mg/kg dose demonstrated safety for intraperitoneal use, reflecting a decrease in mitotic figure counts and neoplastic cell proliferation rates after three consecutive administrations. Additionally, the 15 and 25 mg/kg doses of S. grantii MEL induced structural modifications in the EST microenvironment in mice. The use of the bioproduct also resulted in increased microvascular density and a reduction in the amount of type I and III collagen fibers in intra- and peritumoral regions, significant at 14 and 21 days. It was concluded that S. grantii MEL constitutes a promising source of compounds with antitumor activity. Therefore, it is concluded that the methanolic latex extract of Synadenium grantii represents a promising source of compounds with antitumor activity, exhibiting a dose-dependent antiproliferative effect. However, this formulation induces significant histological changes in organs such as the liver and kidneys, in addition to demonstrating pro-angiogenic effects in the long term.Item Investigação de Salmonella sp. em amostras de suínos abatidos sob inspeção e determinação de seu perfil de resistência aos antimicrobianos(Universidade Federal de Goiás, 2014-06-27) Oliveira, Fábio Henrique de; Sobestiansky, Jurij; Andrade, Maria Auxiliadora; Andrade, Maria Auxiliadora; Bueno, Cláudia Peixoto; Parente, Leila Maria Leal; Pascoal, Lívia Mendonça; Matos, Moema Pacheco ChediakEmbargadoItem Prevenção de problemas colibacilares no início da fase de terminação em suínos mediante a vacinação com colidex-c®(Universidade Federal de Goiás, 2012) Pascoal, Lívia Mendonça; Sobestiansky, Jurij; Chediak, Moema Pacheco Matos; Nunes, Romão da Cunha; Nunes, Romão da Cunha; Vidal, Manuel Guilhermo Ramis; Miguel, Marina Pacheco; Duarte, Sabrina Castilho; Lopes, Eurípedes Laurindo-Item Melia azedarach: influência biogeográfica de diferentes populações oriundas do Estado de Goiás sobre a eficiência carrapaticida e ação sobre a ecdise e oogênese em rhipicephalus microplus (acari: ixodidae)(Universidade Federal de Goiás, 2012-08-10) Sousa, Lorena Alessandra Dias de; Sabóia-Morais, Simone Maria Teixeira de; Ferri, Pedro Henrique; Borges, Lígia Miranda Ferreira; Borges, Lígia Miranda Ferreira; Arruda, Walquíria; Pereira, Débora; Gomes, Abraão Garcia; Silva, Andréa Caetano daEmbargadoItem Aspectos anatômicos dos músculos caudais e mediais da coxa do quati (Nasua nasua, Linnaeus, 1766)(Universidade Federal de Goiás, 2014-05-30) Guimarães, Flávio de Rezende; Cardoso, Júlio Roquete; Araújo, Luciana Batalha de Miranda; Araújo, Eugênio Gonçalves de; Araújo, Eugênio Gonçalves de; Severino, Renato Souto; Pereira, Kléber Fernando; Silva, Marcelo Seixo de Brito e; Cruz, Viviane SouzaSouth american carnivores, members of the Procyonidae family, the coatis (Nasua Nasua) are generalist omnivores that found on fruits the important component for their diet. Thereby, they forage not only on the ground but also in the trees, showing themselves to be skilled climbers. This skill requires more strength and mobility of their hindlimbs when compared to those of their closest relatives, the dogs, with whom they share the same Suborder Caniformia. Thus, it was examined the anatomical aspects of the muscles that integrate the caudal and medial muscular groups of the thigh of coatis, which had its general aspects, location, shape, origin, insertion, syntopy and basic actions evaluated. Both hindlimbs of five adult animals (two females and three males) donated by IBAMA GO (License: 98/2011) were used in this study, which has its protocol approved by the CEUA-UFG (protocol 235/2011). Of these, three animals were euthanized (SISBIO/ ICMBio license: 26278-3). They were fixed with 10% formaldehyde solution and stored in tanks within the same solution, in which they remained for at least 72 hours before the beginning of dissection procedures. Subsequently, they were kept in tanks with ethylic alcohol at 92.8º GL. This doctorate thesis generated two scientific articles. In the first one titled ANATOMICAL ASPECTS OF THE CAUDAL THIGH MUSCLES OF COATI (Nasua nasua, LINNAEUS 1766), it was verified that muscles which comprise the caudal muscular group of the thigh of coatis are the m. semitendinosus, m. semimembranosus, m. biceps femoris and the m. abductor cruris caudalis. The m. semitendinosus has two heads, long and short, besides an intersection tendon. It does not contribute to the formation of the common calcaneal tendon. The m. semimembranosus is incompletely divided into cranial and caudal parts. The m. biceps femoris has only one head and also does not contribute to the formation of the common calcaneal tendon. The ligamentum sacrotuberale is absent and the m. abductor cruris caudalis originates from the deep surface of the m. gluteofemoralis and adjacent part of the m. gluteus superficialis. The second article titled ANATOMICAL ASPECTS OF THE MEDIAL THIGH MUSCLES OF COATI (Nasua nasua, LINNAEUS 1766), it was verified that muscles which comprise the medial muscular group of the thigh of coatis are the m. gracilis, m. pectineus, magnus, brevis and longus adductors muscles and the m. external obturator. All these muscles show particularities regarding to its origin and/or insertion. The m. gracilis is very broad and does not contribute to the formation of the common calcaneal tendon. The m. pectineus extends up to the half of the caudal surface of the femur. The three individual adductors are arranged like three layers that increase craniocaudally, being the adductor brevis positioned between the longus cranially and the magnus caudally. Several anatomical changes were observed in the caudal and medial thigh muscles of coatis in relation to domestic dogs muscles, with some of these, however, resembling more the anatomic patterns of felids. Probably these changes are related to the climbing skills acquired by the coatis during their evolutionary process.Item Qualidade da carne de rãs (Lithobates catesbeianus) suplementadas com óleo de peixe na fase de engorda(Universidade Federal de Goiás, 2014-10-14) Floriano, Luciane Sperandio; Nicolau, Edmar Soares; Padua, Delma Machado Cantisani; Lage, Moacir Evandro; Lage, Moacir Evandro; Castro, André Luis da Silva; Navarro, Rodrigo Diana; Prado, Cristiano Sales; Paula, Fernanda Gomes deEmbargadoItem Aspectos histológicos de órgãos do sistema reprodutor feminino e glândula mamária de quati (Nasua nasua, Linnaeus 1766)(Universidade Federal de Goiás, 2014-06-30) Saddi, Thelma Michella; Araújo, Eugênio Gonçalves de; Santos, Fernanda Cristina Alcantara dos; Brito, Wilia Marta Elsner Diederichsen de; Brito, Wilia Marta Elsner Diederichsen de; Oliveira, Sérgio Marcelino de; Blanck, Lara Silvia Corradi Ollê; Cardoso, Júlio Roquete; Matos, Moema Pacheco ChediakEmbargadoItem Avaliação do potencial de javalis (Sus scrofa) como hospedeiros amplificadores de Rickettsia rickettsii para o carrapato Amblyomma sculptum(Universidade Federal de Goiás, 2025-03-14) Neves, Lucianne Cardoso; Labruna, Marcelo Bahia; http://lattes.cnpq.br/9096422507543208; Monteiro, Caio Márcio de Oliveira; http://lattes.cnpq.br/2496419306394657; Krawczak, Felipe da Silva; http://lattes.cnpq.br/6875905418744479; Krawczak, Felipe da Silva; Hernandez, Alejandro Ramirez; Ueno, Tatiana Evelyn Hayama; Santos, Adriano Pinter dos; Luz, Hermes RibeiroEmbargo.Item Helmintos gastrintestinais de bovinos no Brasil: prevalência, impacto no ganho de peso e efeito do tratamento com lactonas macrocíclicas na produção de ovos de estrongilídeos(Universidade Federal de Goiás, 2023-03-10) Zapa, Dina Maria Beltran; Teixeira, Weslen Fabrício Pires; Felippelli, Gustavo; Lopes, Welber Daniel Zanetti; http://lattes.cnpq.br/4480577038117234; Lopes, Welber Daniel Zanetti; Borges, Fernando Almeida; Amarante, Alessandro Francisco Talamini do; Pereira Júnior, Ronaldo Alves; Bastos, Thiago Souza AzeredoEmbargado.Item Otimização e validação de método bioanalítico para determinação do canabidiol em plasma e obtenção de parâmetros farmacocinéticos em diferentes modelos animais(Universidade Federal de Goiás, 2024-03-28) Santiago, Nathânia Rodrigues; Cunha, Luiz Carlos da; Graziani, Daniel; Martins, Danieli Brolo; http://lattes.cnpq.br/2159963349521553; Martins, Danieli Brolo; Costa, Renata Mazaro e; Sgobbi, Lívia Flório; Cruz, Alessandro de Carvalho; Salazar, Vania Cristina RodríguezIt has dozens of substances called phytocannabinoids, including cannabidiol, a non-psychoactive phytocannabinoid with several therapeutic activities described.. In this sense, the objective of this work was to develop a reliable, economical and robust bioanalytical method to evaluate the pharmacokinetic parameters of cannabidiol in plasma of Wistar rats and dogs. The samples were then evaporated and reconstituted in methanol and formic acid and injected into an HPLC system. Separation was performed using an ACE® C18 column (100 mm × 4.0 mm, 5 μm) at 35 ◦C with isocratic elution using an aqueous phase A (10 mM ammonium formate) and an organic phase B (0.1% methanol/formic acid) (10:90 v/v) under a flow rate of 0.6 mL/min and injection volume of 10 μL. This new method showed linearity in the range of 1–5,000 ng/mL and a lower limit of quantification (LLOQ) of 1 ng/mL, which is comparable to the previously reported LC–MS/MS methods. Inter- and intraday precision and accuracy were less than 15% of DSR and DO, respectively. To demonstrate the suitability of the method for in vivo studies in rats, the assay was applied to a preliminary pharmacokinetic study following IV administration of 10 mg/kg CBD and 20 mg/kg orally. Pharmacokinetic analysis revealed the following: t1/2 CBD, 4.56 h (i.v.) and 3.27 h (p.o). The mean absorption time of oral CBD was 0.08 h, indicating that injectable CBD is primarily absorbed within one hour. Clearance (CL) and volume of distribution (Vd) were 4.8 L·h−1 kg−1 and 33.3 L·kg−1. In conclusion, a simple, sensitive, and cost-effective LC/MS-MS method for CBD determination has been developed, validated, and successfully applied to a pharmacokinetic study in rats.Item Sistema de gestão da qualidade em laboratório oficial de análises de alimentos da Agência Goiana de Defesa Agropecuária: inovação e internacionalização para governança estadual(Universidade Federal de Goiás, 2024-04-30) Gonçalves, Pryscilla Vanesa Rodrigues; Melo, Cristiano Barros de; http://lattes.cnpq.br/1686704472655874; Benício, Cristyene Gonçalves; http://lattes.cnpq.br/0465149436149457; Rezende, Cíntia Silva Minafra e; http://lattes.cnpq.br/5841210447886226; Rezende, Cíntia Silva Minafra e; Martins, Danieli Brolo; Alves, Cláudia Bueno; Guimarães, Marcelo Sales; Fernandes, Daiana de SouzaAgricultural Defense is responsible for ensuring the origin, conformity and safety of products of animal origin intended for human consumption. Food safety depends on compliance with ood manufacturing practices, inspection and correct application of technical norms and standards established in the country. Official laboratory analysis constitutes a relevant regulatory instrument, supporting inspection actions through product monitoring. The lack of management in testing laboratories generates uncertainty regarding the carrying out of activities and results in results with a lower degree of reliability and lack of traceability. To this end, its implementation transforms the analytical rite, assessing innovative management and internationalization of the laboratory ecosystem. In this sense, the objective of this study was to describe the process of implementing a Quality Management System in an official state laboratory for analyzing foods of animal origin, in accordance with the International Organization for Standardization (ISO) 17.025-2017. Initially, a situational diagnosis was carried out in the laboratory intended for this purpose, linked to the government of the state of Goiás named the Food Quality Control Laboratory (LABQUALI); In a second stage, the viability of the laboratory management system (QMS) in the current laboratory structure and the public administration's adherence to execution and restructuring were assessed. The third stage involved the implementation of the QMS and the fourth stage, the critical analysis of the consistency of previous actions for the effectiveness of the new laboratory management. All instruments for enabling the ISO 17.025-2017 standard were declared as necessary requirements and awareness was raised by the state Agricultural Defense Agency of Goias (AGRODEFESA) for the implementation and implementation of the standard. Documents, records were created and prepared, internal documents were issued. There was a broad flow of external documents, creation of manuals, writing of administrative, managerial and technical reports. For physical and financial execution, a schedule for acquisitions, human resources training, internal and external audits, proficiency tests, statistical process control and adaptation of all physical infrastructure and administrative, technical and scientific structure were prepared in relation to the requirements of the General Coordination of Accreditation (CGCRE) from the National Institute of Metrology, Quality and Technology (INMETRO). The results obtained attested that the QMS proposed to LABQUALI met the requirements determined by the reference standard and was in line with what was recommended regarding the precepts and intentions for governance of the state of Goiás. In addition to laboratory quality management, it was verified that the communication with the state structure through the insertion of computerization of the laboratory system into the agency's digital platform and innovative action, compared to the other states of the federation and the Federal District. Another important result refers to LABQUALI's adherence to the Goiás Public Compliance Program. It was concluded that the creation of a laboratory management system generated numerous benefits for AGRODEFESA and the state. The existence of responsibility, reliability and traceability actions for the accuracy of results in food analysis, contributing to the growth and qualification of the official inspection service in the state of Goiás, also corroborates risk monitoring. The QMS implementation model for LABQUALI could support other official laboratories seeking qualifications to master laboratory management for regional and national developmentItem Óleos essenciais para controle de carrapatos: revisão das pesquisas com testes em condição de campo e avaliação de eficácia de combinações de carrapaticidas com (E)-cinamaldeído para controle de Rhipicephalus microplus(Universidade Federal de Goiás, 2022-10-27) Gonzaga, Bruno César Ferreira; Marchesini, Paula Barroso Cruz; http://lattes.cnpq.br/2159998919091266; Lopes, Welber Daniel Zanetti; http://lattes.cnpq.br/4480577038117234; Monteiro, Caio Márcio de Oliveira; http://lattes.cnpq.br/2496419306394657; Monteiro, Caio Márcio de Oliveira; Fernandes, Éverton Kort Kamp; Perinotto, Wendell Marcelo de Souza; Ferreira , Lorena Lopes; Tavares, Caio PavãoThe Rhipicephalus microplus tick was originally found infesting ruminants in South Asia and transported on zebu cattle to other locations in the world. This arthropod causes direct damage to the hosts, being the biggest cause of economic losses in livestock worldwide. In Brazil, with 224.6 million cattle, estimates point to losses of more than 3 billion dollars annually. The control of this parasite is carried out almost exclusively with synthetic acaricides. However, the constant and sometimes incorrect and indiscriminate use of these products can accelerate the selection of resistant ticks. Research to develop technologies to control ticks has been carried out, such as the use of vaccines, biological control agents and essential oils (EOs). Cinnamon (Cinnamomum zeylanicum) EO and its derivative (E)-cinnamaldehyde have acaricides activities. Some EOs and compounds present in EOs (CEOs) showed good acaricide results, including the association between them and synthetic products in vitro and in vivo studies, but these studies are scarce. The present study reviewed the literature with EOs and CEOs for field and semi-field tick control between the years 1991 and 2021 and investigated the effect of the combination of (E)- cinnamaldehyde and amitraz in the laboratory and in the field to control R .microplus. Twentyeight studies were selected in the review, of which 53.6% were carried out in Brazil, 71% with the application of EOs and CEOs in animals and 29% in the environment. In these studies, the spray application method was the most used (75%). The bovine species was the most studied (70%) and the tick R. microplus the most researched (46.4%). From this field and semi-field review, we realized the still limited number of articles evaluating the use of EOs and CEOs, and further studies under these conditions are needed to assess the real potential of these substances for tick control. In this sense, some aspects need to be prioritized: the characterization of the EOs, the standardization of the methodologies used in the efficacy evaluation tests, the use of EOs and COEs combined with synthetic acaricides (looking for synergistic interactions), the development of formulations and the evaluation of the application safety for animals, humans and environment. In vitro tests were carried out with 116 populations of ticks from all regions of Brazil, with 14 synthetic products, and those with 50-80% efficacy was chosen, having only one active ingredient. Subsequently, the products based on amitraz and chlorfenvinphos were tested in binary combinations with (E)-cinnamaldehyde in a tick strain, called CM strain, which showed better results for amitraz. The best association of (E)-cinnamaldehyde and amitraz was used in pharmacotechnical studies for the development and characterization of formulations, evaluating the in vitro and in vivo efficacy to control R. microplus infestations and clinical safety for cattle. The approach of combining this CEO and the synthetic product improved the effectiveness of the latter (reaching 86%) in engorged females (in vitro), but in the field study with application on animals, (E)-cinnamaldehyde caused intoxication in cattle. Future research, seeking to reduce the toxicity of formulations with (E)-cinnamaldehyde, deserves to be investigated.Item Desenvolvimento de sorvete funcional simbiótico com polpa de cagaita (Eugenia dysenterica)(Universidade Federal de Goiás, 2021-12-17) Pfrimer, Renata Teixeira; Cordeiro, Clarice Gebara Muraro Serrate; http://lattes.cnpq.br/5073363015303780; Coelho, Karyne Oliveira; http://lattes.cnpq.br/4493179418459800; Nicolau, Edmar Soares; http://lattes.cnpq.br/9601723963736071; Nicolau, Edmar Soares; Fonseca, Leorges Moraes da; Lage, Moacir Evandro; Antunes, Veridiana de Carvalho; Cavicchioli, Valéria QuintanaBio-inspired technological innovation in ice cream manufacturing has driven the market for dairy products. In this way, increasing the nutritional density and approving ice cream, with the use of whey, buttermilk, inulin, probiotic and fruit from the Cerrado are viable alternative for the sector. The objective was the production and physicochemical, microbiological and sensory characterization of different ice creams, varying those due to the milk/cream ratio, whey and buttermilk defined by the Simplex Centroide Design, and subsequently added a probiotic strain of Lactobacillus acidophilus, inulin and cagaita pulp (Eugenia dysenterica). Nine ice creams were produced with whey and buttermilk contents, ranging from 5% to 15%, and milk/cream ratio from 70% to 90%, as stipulated by the Simplex Centroide Design. All formulations result within the standards defined by current legislation. Ice creams showed higher content of palmitic, oleic and stearic fatty acids. They stood out as formulations with the highest concentration of co-products for presenting the best nutritional composition, considering the high levels of protein, protein fractions, lipids and lactose. The high concentration of whey and buttermilk concentration, as well as the control formulations, totaling Lactobacillus acidophilus six, were increased with 0.5% of, being three with the addition of 20% of cagaita pulp and three without the addition of pulp. Subsequently, all formulations were increased with 5% of inulin. Physical-composition, microbiological, fatty acid profile, quantitative descriptive analysis, rheology, texture profile analysis and confocal microscopy were performed. All formulations result within the standards defined by current legislation. Available probiotic and symbiotic ice creams were categorized into standard and premium ice creams by fat content. Formulations with cagaita pulp presented higher acidity, moisture and lower melting speed and higher instrumental hardness. The ice creams exhibited equivalence in relation to the semiquantification of image pixels, accompanied by confocal microscopy analysis, with the quantification of lipids and proteins. Ice cream with the addition of pulp, presented higher intensity of yellow color and citrus aroma. All ice creams are categorized as probiotic foods and source count 8 Log10 CFU / g, being great vehicles to provide probiotic culture and all its benefits when consumed.Item Estudo macroscópico do telencéfalo, da vascularização da base e seios da dura-máter em Alouatta belzebul(Universidade Federal de Goiás, 2020-11-11) Pereira, Dayane Kelly Sabec; Pereira, Kleber Fernando; http://lattes.cnpq.br/4779531102983799; Vulcani, Valcinir Aloisio Scalla; http://lattes.cnpq.br/9821938265591545; Vulcani, Valcinir Aloisio Scalla; Franzo, Vanessa Sobué; Silva, Ana Paula Sousa Paixão Barroso da; Ramos, Luana dos Anjos; Birck, Arlei JoséPrimates of the species Alouatta belzebul, also known as howler monkeys, guariba and barbado are endemic to Brazil, of large size, slow movements and quadrupedal locomotion. The knowledge of the morphology of this species is scarce and, in some aspects, as the brain is non-existent. The objective was to describe the macroscopic anatomy of Alouatta belzebul's telencephalon, as well as the vascularization of the base of the brain, nuclei of the base and the venous sinuses of the dura mater. Twenty specimens of Alouatta belzebul were studied, where the brain gyres and grooves were dissected, the base nuclei were identified and the arterial system and venous sinuses were perfused with the injection of colored latex. The telencephalon of A. belzebul presented lysencephalic characteristics, corroborating with several other species of non-human primates and differing from genera such as Pan and Homo. The nuclei of the base were quite evident and the caudate nucleus, putamen, pale medial globe and pale lateral globe, cloister and black substance have been described, which are functionally related to the motor behavior of the species. In the analysis of the encephalization index, it was observed that the Alouatta belzebul is phylogenetically closer to Sapajus and Macaca and more distant from genera such as Brachyteles and Callithrix, showing expressive cognition and intelligence. Regarding the inclination of the central groove, it was observed that in Alouatta belzebul the upper extremity is posterior to the lower extremity, data that corroborate with man, baboons and chimpanzees and reveal a large frontal lobe when compared to Sapajus libidinosus, showing the maximum development in these primates. The arterial circle of the Alouatta belzebul consists of two vascular systems: the vertebro-basilar and the carotid system, which anastomose to close the arterial circuit. In the caudal portion of the arterial circle are the vertebral arteries and their branches: the rostral spinal artery and the caudal inferior cerebellar artery. Anastomosis of the vertebral arteries gives rise to the basilar artery. This presented an anatomical variation with the formation of a double basilar artery, called the arterial island. Nine venous sinuses were observed in the dura mater: dorsal sagittal sinus, ventral sagittal sinus, transverse sinus, straight sinus, sigmoid sinus, temporal sinus, parietal sinus, basilar sinus and cavernous sinus, with morphological similarities in the origin, path and destination of the flow blood to the internal jugular vein, helping in the venous drainage function of the brain in this species. Of the venous sinuses observed, the cavernous sinus was of considerable clinical and surgical importance in Alouatta belzebul due to its topographic arrangement next to the internal carotid artery and pituitary gland, data similar to those described for Sapajus libidinosus, Macaca fascicularis, Macaca mulatta, Papio ursinus, Cercopithecus pygerithrus and Galago senegalensis. The morphological study of the telencephalon, as well as the mechanisms of lining, arterial irrigation and drainage of the venous sinuses, generated information about the brain organization of the primate Alouatta belzebul, which had not been previously described and many of these data generate subsidies for understanding other áreas ethological investigations.Item Avaliação histológica, microestrutural e de biocompatibilidade in vivo de cartilagens auriculares de bovino tratadas em solução alcalina(Universidade Federal de Goiás, 2021-03-05) Ferreira, Kamilla Dias; Silva, Luiz Antônio Franco da; http://lattes.cnpq.br/0446055905975647; Vulcani, Valcinir Aloisio Scalla; http://lattes.cnpq.br/9821938265591545; Vulcani, Valcinir Aloisio Scalla; Silva, Danilo Conrado; Martins, Apóstolo Ferreira; Franzo, Vanessa Sobue; Oliveira, Kellen de SousaWith the advancement of techniques for the correction of tissue defects or losses, the use of biomaterials that promote repair with a minimum of inflammatory response is sought. Previous studies carried out used different means of preparing and obtaining elastic bovine cartilage, aiming at the use as biomaterial. However, one of the major challenges related to the use of these membranes, refers to the occurrence of unwanted immunogenic reactions at the implantation site. This study aimed to characterize the physical-chemical, microstructural and histological properties of elastic bovine cartilages treated in alkaline solution and their biocompatibility in vivo. Therefore, physical-chemical analyzes were carried out to characterize this material. The thermal analyzes performed, Thermogravimetry (TGA) and Differential Exploratory Calorimetry (DSC), verified the possible changes in the material in view of the temperature variations in which it was submitted. The TGA curves showed the variation in the mass of treated and untreated auricular cartilages as a function of temperature and in the DSC the temperature of the collagen denaturation of the cartilage samples was evaluated. The analyzes showed that the treated cartilage had physicochemical characteristics similar to untreated cartilage. In the microstructural evaluations, 2D and 3D computed microtomography and confocal laser scanner microscopy were performed. Two-dimensional Micro-Ct showed that the cartilage region showed higher density in relation to the perichondrium, and the alkaline treatment was effective in decellularization, due to the presence of gaps observed in the extracellular matrix interspersed with the collagen structure. The three-dimensional Micro-Ct showed that the cartilage has less porosity and pores with a larger diameter and in the laser scanner microscopy it was noted that the treated cartilage has considerable roughness, factors that can contribute to cell proliferation and adhesion. In this study, histological processing of cartilage was also performed, demonstrating that the alkaline treatment promoted tissue decellularization, with the maintenance of the architecture of the extracellular matrix and the structure of elastic and collagen fibers. It was concluded that the alkaline treatment was efficient to promote decellularization in the auricular cartilage. The last stage of the study consisted of evaluating the in vivo biocompatibility of elastic cartilage implants treated with alkaline solution (CA) compared to polypropylene (TP) mesh in rabbits. It was found that the group (CA) had a less intense inflammatory process than the group (TP), in which the formation of a fibrous capsule around the implants was observed. It was noted in the group (CA) the presence of calcification promoted by osteoinduction of osteoblasts due to alkaline processing, which can be considered a bias of interest for further studies, involving the regeneration of bone tissues, in which the cell-type applicability observed is feasible.Item Aspectos clínico-epidemiológicos e tratamento da tripanosomose bovina por trypanosoma vivax no estado de Goiás(Universidade Federal de Goiás, 2019-03-01) Bastos, Thiago Souza Azeredo; Linhares, Guido Fontgalland Coelho; http://lattes.cnpq.br/6261928164195145; Lopes, Welber Daniel Zanetti; http://lattes.cnpq.br/4480577038117234; Lopes, Welber Daniel Zanetti; Felippelli, Gustavo; Krawczak, Felipe da Silva; Ferreira, Lorena Lopes; Silveira Neto, Osvaldo José daThis study reports the first case of bovine trypanosomiasis caused by Trypanosoma vivax in Goiás, Brazil, as well as other cases that occurred within a period of 24 months, demonstrating epidemiological aspects such as needle sharing and commercialization of infected cows influencing the spread of this disease. Furthermore, it was observed that T. vivax was able to infect and develop the disease in calves when they were inoculated by the intradermal, subcutaneous, intramuscular or intravenous route. The presence of Anaplasma marginale latent in calves with trypanosomiasis interfer directly in the percentage of mortality of the animals. Finally, with regard to efficacy, only animals that received isometamidium were able to remain without new parasitaemia after 180 days of study. Treatment with diminazene allowed new parasitemia when reinfected 50 days after treatment and, finally, imidocarb was not effective in the treatment of bovine trypanosomosis. This is the first study that describes the presence of T. vivax, as well as its epidemiology in the State of Goiás, evaluated the infection capacity of this parasite by different pathways, clinical signs, and diagnostic methods and also evaluated the efficacy of the treatment with imidocarb, diminazene and isometamidium in cattle experimentally infected with T. vivax in Brazil.