Programa de Pós-graduação em Medicina Tropical e Saúde Pública
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Item Envolvimento de células ER-MP58+ na produção de IL-12 em linfonodos drenantes da infecção inicial por leishmania major em camundongos BALB/c(Universidade Federal de Goiás, 2006) Cardoso, Ludimila Paula Vaz; Lima, Glória Maria Collet de Araújo; http://lattes.cnpq.br/9228137287854181; Oliveira, Milton Adriano Pelli de; http://lattes.cnpq.br/2152513705182408The development of a Th1 immune response is the key event to prevent Leishmania infection and is linked with the IL-12 production by monocytes, dendritic cells, macrophages and neutrophils. The IL-12 signal induces the increase of IFN-γ production by T cells, favoring the profile of the Th1 immune response and the resistance phenotype to the infection. In the vertebrate host, the leishmania inhabits in cells of the mononuclear phagocyte system (MPS), which is also responsible for the elimination of the parasite. The participation of immature cells of MPS in the initial production of IL-12 has been discussed. It was demonstrated recently that a population of mononuclear phagocytes, derived from bone marrow culture and expressing the marker ER-HR3 produced great amount of IL-12p40 after stimulation in vitro with procyclic or metacyclic promastigotes of L. (L.) major. The initial production of IL-12 by MPS cells is under investigation. It is unclear the role of mononuclear phagocytes, in different stages of maturation, in the production of IL-12p40 in vivo. In this work, we evaluated the involvement of MPS cells, in different stage of maturation, with the IL-12p40 production in vivo in draining lymph nodes of BALB/c mice after 48 h of infection with L. (L.) major. Our results showed that CD31+ cells (mononuclear phagocytes precursors) were absent in the draining lymph nodes of the subcutaneously footpad injection and that the number of ER-MP58+ (immature mononuclear phagocytes), ER-HR3+ cells (mononuclear phagocytes in the intermediate stage of maturation) and 33D1+ (mature phagocytes) increase in the period of 48 h after infection. We showed, also, that ER-MP58+ responsible for great part of the draining lymph nodes IL-12 production 48 h after infection with stationary-phase promastigotes of L. (L.) major in mice BALB/c. We suggested that, in vivo, the ER-MP58+ population, together with other cellular populations, known as IL-12 producers, ER-MP58+ cell has an important role in the inflammation induced by the parasitic inoculum.Item Colonização pelo streptococcus do grupo b: prevalência, fatores de risco, características fenotípicas e genotípicas, em mulheres no terceiro trimestre de gestação, atendidas por serviço de referência materno infantil de Goiânia-Goiás(Universidade Federal de Goiás, 2009) Pires, Telma Sousa; André, Maria Claúdia Dantas P. B.; Turchi, Marília Dalva; http://lattes.cnpq.br/3769826743537934To estimate the prevalence, to asses risk factors for Group B Streptococcus (GBS) colonization and to describe phenotypic and genotypic characteristics of isolated strains from pregnant women in Goiânia, Goiás. Methods: A cross sectional study was carried out among 198 pregnant women, at least at the 32o weeks´ gestation, attending a reference health unit, from March to June 2009. Socio, demographic and obstetric profiles were investigated using a standard questionnaire. Samples of vaginal and rectal secretion were collected and placed into selective enrichment broth Todd-Hewitt. Tests for GBS identification (gram, catalase and CAMP) followed by susceptibility test using antibiotic disk diffusion technique were performed. Genetic diversity was assessed by pulsed-field gel electrophoresis (PFGE). Descriptive and analytic statistical tests were applied (Epi Info e SPSS 13.0). Analyses were performed at the Instituto de Patologia Tropical Saúde Pública/UFG. Results: Thirty pregnant women were colonized by GBS yielding a prevalence of 15.2% (IC95% 10.5 ?? 20.9). Pregnant women younger than 20 years and with low income had higher risk of GBS colonization, in univariate analysis (p<0.05). GBS was isolated from 28 vaginal and 14 rectal specimens. Twelve pregnant were vaginal and rectal colonized. All 42 strains were susceptible to penicillin, vancomycin, ceftriaxone and levofloxacin. Three strains (7.1%) were resistant to erythromycin and two (4.7%) to clindamycin. 19 pulsotypes and four clusters were identified. Nine out 12 pars of positive strains (vaginal and rectal) were genetically identical, two were stricted related and one par was colonized by different strains. The same genetic profile was observed in more than one pregnant. Conclusions: Socioeconomic and obstetrics variables had low predictive value for GBE colonization among pregnant women, reinforcing the need for universal microbiology screening strategy in this population, in order to prevent neonatal sepsis. A high genetic diversity of GBS was found among pregnant women in Goiania.Item Pesquisa de anticorpos IgG séricos anti-lipoproteínas de mycoplasma fermentans e mycoplasma hominis ou anti-mam (superantigeno de mycoplasma arthritidis) em pacientes com artrite reumatoide ou lupus eritematoso sistemico(Universidade Federal de Goiás, 2008) Rocha Sobrinho, Hermínio Maurício da; Dias, Fátima Ribeiro; http://lattes.cnpq.br/5741031258926403Rheumatoid Arthritis (RA) and Systemic Lupus Erythematosus (SLE) are autoimmune diseases of unknown etiology. Some species of mycoplasmas cause arthritis in animals and humans, and their lipid-associated membrane proteins (LAMPs) and Mycoplasma arthritidis mitogen (MAM superantigen) are potent stimulators of the immune system. Thus, it has been proposed that mycoplasma can be involved in autoimmune-disease etiology. The objective of the present work was to detect antibodies to MAM and LAMPs of M. hominis and M. fermentans in the patient sera, and to characterize the profile of IgG antibodies reactivity with LAMPs in order to identify the major immunogenic mycoplasmal lipoproteins that could be involved in the etiopathogenesis of these autoimmune diseases. Serum samples were obtained from peripheral blood of female patients at the same age of healthy controls. Recombinant MAM (from M. arthrititidis), LAMPs of M. hominis PG21 and M. fermentans PG18 were used in Western blotting assays. Antibodies to MAM were detected in the patient and control sera (RA: 27.5% vs 18.8%; SLE: 21.7% vs 20.0%). At least 23 LAMPs were found in the preparations of M. hominis PG21 and of M. fermentans PG18 with molecular masses between 20 and 192 KDa. The sera of RA patients recognized a larger number of LAMPs of M. hominis PG21 and M. fermentans PG18 than the control sera (RA: 11 ± 4 vs controls: 7 ± 3, n = 35; p < 0,05). Most of the sera of RA patients presented strong reactivity with LAMPs of M. hominis PG21 (RA: 65.7% vs controls: 20%, p < 0.05). LAMPs of M. hominis PG21 with molecular masses < 49 and ? 20 KDa and LAMPs of M. fermentans PG18 < 102 and ? 58 were mainly recognized by IgG antibodies of RA patients. When comparing sera from SLE patients and controls there was detected no significant differences between the profiles of IgG reactivity. Therefore, M. hominis PG21 LAMPs (< 49 and ? 20 KDa) and M. fermentans PG18 LAMPs (< 102 and ? 58 KDa) are high immunogenic mycoplasmal antigens that can induce antibody cross reactivity with self antigen, contributing with the RA pathogenesis.