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Item Avaliação da atividade genotóxica e antigenotóxica de Celtis iguanaea (Jacq.) Sargent em bactérias e camundongos(Universidade Federal de Goiás, 2010-02-24) Borges, Flávio Fernandes Veloso; Lee, Chen Chen; http://lattes.cnpq.br/4621907105842007Celtis iguanaea (Jacq.) Sargent is a member of the Cannabaceae family and popularly known as esporão de galo. Ethnobotanical surveys of cerrado native plants show that the tea leaves of C. iguanaea is used in traditional medicine for body aches, asthma, cramps, poor digestion, urinary infections, kidney disfunctions, as a stimulant or as a diuretic.The purpose of the present work was to evaluate the possible cytotoxic, genotoxic and antigenotoxic effects of the leaves aqueous extract of C. iguanaea by the prophage λ induction test (SOS Inductest) and the micronucleous test in mice bone marrow. The SOS Inductest was performed according to Moreau (1976), using lysogenic WP2s(λ) and indicator RJF013 strains derived from Escherichia coli. WP2s(λ) cultures were treated with different doses of the extract (0.5, 1, 2.5, and 5 mg) (evaluation of genotoxicity) or co-treated with a single dose of MMC (0,5 mg) and different doses of extract (assessment of antigenotoxicity). Then, the treated culture was added to the indicator strain (RJF013) and both were poured on plates in LB(1/2) medium. For the evaluation of cytotoxicity, cultures of WP2s(λ) treated with different doses of extract were diluted in M9 buffer and plated on LB. The micronucleus test was carried out according to Schmid (1975). To assess the genotoxic activity, animals were treated with Celtis iguanaea extract (100, 300 and 500 mg/kg concentrations). To evaluate the antigenotoxic activity, the same doses of extract were treated simultaneously with 4mg/Kg of MMC. The frequencies of micronucleated polychromatic erythrocytes (MNPCE) were evaluated at 24h and 48h exposure period except the negative control (24h). The cytotoxicity was assessed by the polychromatic and normochromatic erythrocytes ratio (PCE/NCE). In both tests the obtained results demonstrated anticytotoxic activity and absence of genotoxicity and cytotoxicity of the Celtis iguanaea leaves extract. The extract also showed partial antigenotoxic activity in bacteria and antigenotoxic activity in mice.Item Efeito anti-proliferativo de curcumina associada á nanopartículas magnéticas funcionalizadas com bicamada de ácido láurico sobre células de melanoma humano skmel 37(Universidade Federal de Goiás, 2011) Souza, Fernanda França de; Guillo, Lídia Andreu; http://lattes.cnpq.br/3401436781775091Curcumin has emerged as a great promise for cancer treatment and chemoprophylaxis with curcumin, but its low solubility in water is minimal systemic bioavailability. Attempts were made to improve its solubility in aqueous solutions by incorporating curcumin in liposomes or micelles, but these systems have low stability. In addition, magnetic nanoparticles have been used as promising drug delivery systems because they can be functionalized to become water soluble and biocompatible. Studies with curcumin incorporated into the magnetic nanoparticles are still scarce. This study aims to evaluate the antiproliferative effect of curcumin combined with magnetic nanoparticles functionalized bilayer of lauric acid in human melanoma cell line SKMEL 37. Human melanoma cells were treated at different concentrations and cytotoxicity was evaluated by MTT assay. The cell morphology was evaluated by light microscopy and fluorescence. Our studies have shown that curcumin has incorporated into nanoparticles cytotoxic effect at concentrations above 40,8 μg/ml or 111 μM. Since free curcumin caused significant death at concentrations above 11.5 μM. We also observed morphological changes typical of apoptosis such as chromatin condensation, nuclear fragmentation and bleb formation. These findings show us that both their cytotoxic activity, as both the magnetic properties and its solubility in water make this new formulation with a curcumin compound interest for therapeutic use.