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Item Aditivos antimicrobianos e processamento de grão na terminação bovinos de corte confinados(Universidade Federal de Goiás, 2017-02-17) Camilo, Fernando Rossi; Siqueira, Gustavo Rezende; http://lattes.cnpq.br/9873354467031857; Fernandes, Juliano José de Resende; http://lattes.cnpq.br/7949776443064259; Fernandes, Juliano José de Resende; Silva, Rodrigo Medeiros da; Oliveira Júnior, Reinaldo Cunha de; Pádua, João Teodoro; Ribeiro, Marinaldo DivinoThe experiment 1 objectives were to evaluate the isolated and combined effects of the virginiamycin (VM) and monensin sodium (MON) on performance, feed intake, feed efficiency, ruminal fermentation, and carcass characteristics of beef cattle fed with high concentrate diet, the experiment 2 had the objective evaluate ruminal parameter the bulls Nelore with use the isolated and combined effects of the virginiamycin (VM) and monensin sodium (MON) and the experiment 3 had the objective to determine the optimal moisture content for reconstituted ensiled corn grain, evaluating final moisture concentrations of 27%, 30%, 33%, and 36%. In Exp. 1, 339 crossbred bulls Nellore x Guzera; were used in a randomized complete block design with five treatments and seven replicates. The blocks were defined by initial body weight. The animals were allocated in group pens for 103d, including 28d adaptation period. Treatments were defined by levels of VM and MON (mg/kg of DM) as follows: 30MON; 15VM+30MON; 25VM+30MON; 34VM+ 30MON e 34VM. In the phase of adaptation was no statistical (P>0.05) in body weight initial, body weight final, and average daily gain. Already to dry matter intake have the statistical difference (P<0.05) to compare 30MON vs 34MON e 34VM vs 34VM+30MON, in the phase adaptation the dose association 34VM+30MON have the smaller dry matter intake. In Exp. 2, 15 Nellore bulls with ruminal cannula were used in a randomized complete block design with 5 treatments and 3 replicates. Animals were blocked by initial body weight. The animals were kept in individual pens for 35 days has received the same diet to Exp.1. The sample liquid ruminal was collected in days 0, 7, 14, 21, and 28 for evaluation pH, VFA, N-NH3, and protozoan. After this period was realized the digestibility. The values of pH, NH3-N, and VFA were no differences (P>0.05) for contrast and linear and quadratic regression, except for acetic acid concentration that was difference (P<0.05). The protozoa concentration total had effect in contrast 34VM vs 34VM+30MON (P<0.05), being that the dose 34VM provide more concentration. In the Exp.3 sixty cattle (Bos taurus) were randomized complete block design with 6 treatments and 10 replicates was used. The treatments were corn grain reconstituted with water until they reach to final moisture concentrations of 27 (HMC27%), 30 (HMC30%), 33 (HMC33%), and 36% (HMC36%) and grains were allowed to ensile for 101 days. The steam-flaker corn and dry-rolled corn also were usually with treatment. Were no statistical difference (P<0.05) in performance the cattle confined for 89 days. Already in experiment in vitro the gas production the high moisture corn. To usually the high moisture corn in diet the cattle no increased the performance. Already the processing corn were availed in vitro, high moisture result in high fermentation and gas production.Item Aditivos antimicrobianos e inclusão de fibra em dietas de milho grão inteiro para bovinos de corte(Universidade Federal de Goiás, 2016-03-09) Lemos, Barbara Juliana Martins; Castro, Flávio Geraldo Ferreira; http://lattes.cnpq.br/7651676206685012; Couto, Victor Rezende Moreira; http://lattes.cnpq.br/4329309889502866; Fernandes, Juliano José de Resende; http://lattes.cnpq.br/7949776443064259; Fernandes, Juliano José de Resende; Ferreira, Reginaldo Nassar; Oliveira Júnior, Reinaldo Cunha de; Lima, Milton Luiz Moreira; Melo, Heloisa Helena de CarvalhoThe objective of this study was evaluate the effects of antimicrobial feed additives and inclusion of pelleted sugarcane crop residue (SCR) as a fiber source in whole shelled corn (WSC)-based finishing diets (85% WSC + 15% pelleted concentrate, DM basis) on growth performance, nutrients digestibility, rumen fermentation and eating behavior of zebu cattle. Growth performance trials were conducted in randomized block design, and metabolical trials were conducted in Latin Square design using ruminally fistulated steers. In the antimicrobial feed additives studies, 100 bulls were fed for 101 d in Exp. 1 to evaluate the treatments M30 (30 mg of monensin [M]/kg DM), V25 (25 mg of virginiamycin [V]/kg DM), M20V25 (M 20 mg/kg DM + V 25 mg/kg DM), F4 (4.4 mg of flavomycin [F]/kg DM), and M20F2 (M 20 mg/kg DM + F 2.2 mg/kg DM); and in the Exp. 2 (Latin Square 7 × 7) were evaluate the same 5 treatments of Exp. 1 plus 2 additional treatments: M30V25 (M 30 mg/kg DM + V25 mg/kg DM) and M20F4 (M 20 mg/kg DM + F 4.4 mg/kg DM). In the SCR studies, 80 bulls were fed diets with 0, 3, 6, and 9% SCR (DM basis; SCR-0, SCR-3, SCR-6, and SCR-9, respectively) for 103 d in Exp. 1; and in the Exp. 2 (5 × 5 Latin Square) were evaluated the inclusion of 0, 3, 6, 9 and 12% of SCR (DM basis; SCR-0, SCR-3, SCR-6, SCR-9, and SCR-12, respectively). In the antimicrobial feed additives studies growth performance (final BW, ADG in live weight basis, DMI, and G:F), carcass characteristics (HCW, ADG in carcass basis, carcass transfer, dressing percent, and 12th-rib-fat), apparent total tract DM, OM, CP, and NDF digestibility were similar among treatments. There were no treatment effects and no interaction of treatment × time in rumen fermentation responses (ruminal pH, rumen ammonia nitrogen, number of protozoa, and VFA). In the WSC study, inclusion of SCR linearly increased daily DMI up to 25% (P = 0.01). Dry matter intake, as percentage of BW, increased (P = 0.01) 0.06 percentage units with each 1% inclusion of SCR. Over the entire finishing period (d 0 to 103), there were trends for linearly increase final BW up to 3.3% (P = 0.10), and ADG up to 10.7% (P = 0.07) in response to dietary SCR levels, consequentially G:F tended (P = 0.07) to linearly decrease up to 10.2% with inclusion of SCR compared with CTL diet. Apparent total tract digestibility linearly decreased (P < 0.01) 30% for DM, 28% for OM, 45% for CP, and 61% for NDF, from CTL for SCR-12. No treatment effect and no interaction of treatment × time were observed on ruminal pH, concentrations of rumen ammonia nitrogen and VFA. Time spent eating, eating rate, and meal frequency were not affected by inclusion of SCR up to 12%. There was a quadratic response on meal size (P = 0.04). In summary, feeding isolated or combined antimicrobial feed additives to zebu cattle resulted in similar responses. Pelleted SCR seemed to be low fermentable and non-effective to stimulate rumination by cattle fed WSC-based finishing diet.Item Facilitando a adaptação de bovinos (Bos taurus indicus) para dietas de confinamento de alta proporção de concentrado utilizando Megasphaera elsdenii(Universidade Federal de Goiás, 2021-05-28) Lopes, Ana Laura Araújo; Fernandes, Juliano José de Resende; http://lattes.cnpq.br/7949776443064259; Fernandes, Juliano José de Resende; Meschiatti, Murillo Alves Porto; Couto, Victor Rezende MoreiraThis study evaluated the effects of Megasphaera elsdenii administration at the beginning of the feedlot period on performance of Bos taurus indicus bulls. On d 0, 383 Nellore bulls (initial shrunk body weight 384 ± 29.2 kg; initial age = 24 ± 2 mo) were assigned to treatments in a randomized complete block design. Treatments consisted of 1) 14 d adaptation diet and transition to a finishing diet (CONT), 2) CONT plus oral administration of 20 mL of Lactipro-NXT (M. elsdenii) on d 0 of the study (MEG-14), 3) CONT diet, consisting of 6 d of adaptation diet plus oral administration of 20 mL of Lactipro-NXT on d 0 of the study (MEG-6), and 4) No adaptation diet and oral administration of 20 mL of Lactipro-NXT on d 0 of the study (MEG-0). Experimental period lasted 119 d. No treatment effects were observed for any of the performance parameters evaluated herein (P ≥ 0.15). Nonetheless, a treatment × wk interaction was observed for DM, NEm, and NEg intakes (P < 0.0001). For all these parameters, MEG-0 and MEG-6 had a reduced intake vs. MEG- 14 and CONT in the first wk of the study (P ≤ 0.05). For the carcass traits, no effects were observed for HCW (P ≥ 0.24), whereas MEG-6 had a greater REA when compared with MEG-0 and MEG-14 (quadratic effect; P = 0.04) and MEG-administered bulls tended to have a greater BFT vs. CONT (P = 0.08). In summary, M. elsdenii administration at the beginning of the feedlot period did not improve performance, whereas reducing the length of the adaptation period for 6 d improved REA of finishing Bos taurus indicus bulls.Item Uso de aditivos e adaptação para dietas com alta inclusão de grão de milho inteiro de bovinos confinados(Universidade Federal de Goiás, 2017-02-17) Mobiglia, Andrea de Mello; Couto, Victor Rezende Moreira; http://lattes.cnpq.br/4329309889502866; Fernandes, Juliano José de Resende; http://lattes.cnpq.br/7949776443064259; Fernandes, Juliano José de Resende; Silva, Rodrigo Medeiros da; Ribeiro, Marinaldo Divino; Oliveira Júnior, Reinaldo Cunha de; Pádua, João TeodoroThe objective was to evaluate the effects of antimicrobial additives and the inclusion of roughage during adaptation period in diet containing whole shelled corn (WSC, 85% WSC + 15% pelleted concentrate) on performance of Zebu bulls (Exp. 1). The Exp. 1 was realized in randomized complete block design with 2x2 factorial and five replicates. A hundred bulls were fed for 105 d with diet containing monensin (MON, 30 mg/kg DM) ou virginiamycin (VM, 25 mg/kg DM) and with or without inclusion of sugarcane bagasse as roughage (ROU or NO-ROU) in adaptation period. The Exp. 2 was parallel conducted in randomized design with two treatments (MON, 30 mg/kg DM or VM, 25 mg/kg DM) and five replicates. Ten fistulated steers were used and fed with the same diet than Exp. 1 without inclusion of roughage. A laboratorial experiment (Exp. 3) was realized at Kansas State University and its objective was to evaluate the capacity of Megasphaera elsdenii (ME) strain 41125 metabolizes a range of alternative substrates as carbon source. The experiment was designed in randomized complete blocks with six replicates. Viable cell counting, pH, and optical density were measured in media containing glucose, fructose, galactose, arabinose, xylose, maltose, sucrose, lactose, lactate, trehalose, raffinose, Fructooligosaccharide, potato starch, soy protein, succinate. In Exp. 1 and Exp. 2, antimicrobial additives, MON and VM, added to diet containing 85% WSC and 15% pellet concentrate had equal results for performance, ruminal fermentation, and apparent total tract digestibility of nutrients (P≥0.05). However, lower intake was observed in animals fed with monensin includes on diet (P≤ 0.027). Although the additive used had no effect on performance on Exp. 1, the inclusion of roughage in adaptation period showed tendentiously greater final body weight, average daily gain, and efficiency (P≤ 0.088; P≤ 0.075 e P≤ 0.094, respectively) for adaptation period (1 to 20 d) and entire feedlot period (1 to 105 d). There were no treatment effects and interactions for carcass characteristics as hot carcass weight, dressing percentage, carcass daily gain (P≥0.05). In Exp. 3, Megasphaera elsdenii was able to metabolize alternative substrates as glucose, maltose, lactate, Fructo-oligosaccharide, and fructose. However, ME had greater growth in media containing fructose, but the viable cell counting did not show consistent results with optical density. Investigations must be done to clarify the ME metabolism when fructose is used as carbon source. Accord to results obtained in this study, a roughage sorce might be included on adaptation period in diet contenting 85% whole flint corn plus 15% concentrate pellete to obtain greater animal performance. Thus, Megasphaera elsdenii is able to use alternative substrates as lactate, glucose, fructose, and maltose, maintaining their population in rumen.Item Efeito do tanino no desempenho e características de carcaça de bovinos não castrados terminados em confinamento(Universidade Federal de Goiás, 2019-03-07) Nascimento, Kaique de Souza; Couto, Victor Rezende Moreira; http://lattes.cnpq.br/4329309889502866; Silva, Maurícia Brandão da; http://lattes.cnpq.br/9608210292530980; Fernandes, Juliano José de Resende; http://lattes.cnpq.br/7949776443064259; Fernandes, Juliano José de Resende; Cabral Filho, Sérgio Lucio Salomon; Restle, JoãoIn order to increase the efficiency of the use of the nutrients supplied to the animals, the use of additives becomes an option because it directly interferes with the ruminal fermentation and consequently can improve the performance of the animals. Antibiotics are the most commonly used additives in feedlots, but over the years the use of antibiotic alternatives has been increasing due to the restriction of some markets to the use of antibiotics in animal production. In this sense, the use of tannins in the nutrition of beef cattle has been gaining strength as an alternative to antibiotics. The objective of this study was to evaluate the performance and carcass traits of steers in the finishing phase, receiving diets with the addition of a commercial product based on tannins or Monensina. A total of 160 Nelore (64) and Angus (96) with mean age of 20 months and initial weight of 342 ± 25 kg were used. The experimental design used was a randomized complete block, being considered as weights and breeds blocks, the experimental unit considered was the pen composed by 8 animals and 5 replicates per treatment, totaling 20 experimental units. The treatments evaluated were: M14 - Diet with 14% of CP and 25 ppm of Monensin; MT14 - Diet with 14% of CP, 25 ppm of Monensin and 0.15%/DM of Tannins; T14 - Diet with 14% of CP and 0.15%/DM of Tannins; MT13 - Diet with 13% of CP, 25 ppmof Monensin and 0.15%/DM of Tannins. The treatments provided the same diet for all animals (90 and 10%, for concentrate and forage, respectively). The experimental period was 105 days with 14 days of adaptation. The treatments MT14 and T14 presented values higher than the others for final weight (P = 0.002), dry matter intake (P <0.001), intake per live weight percentage (P <0.001), total mean gain (P = 0.005) and average daily gain (P = 0.005). Food efficiency (P = 0.157) was similar among all treatments. The parameters of ribeye area (P = 0.332), subcutaneous fat thickness (0.848), gluteus medius fat thickness (P = 0.830) and gluteus medius area (P = 0.141) obtained by ultrasonography showed no difference between the treatments. The final carcass weight (P = 0.075) and carcass ADG (P = 0.076) were higher for the MT14 and T14 treatments compared to the others. Carcass yield did not differ between treatments (P = 0.904). The use of a mixture of condensed and hydrolyzable tannins was shown to be viable in the finishing of beef cattle, presenting feed efficiency and carcass characteristics similar to the monensin additive in diets with 14% and 13% crude protein.Item Substituição da virginiamicina por produtos à base de levedura (Saccharomyces cerevisiae) em dietas de bovinos(Universidade Federal de Goiás, 2020-03-10) Oliveira, Daiana dos Santos de; Couto, Victor Rezende Moureira; http://lattes.cnpq.br/4329309889502866; Fernandes, Juliano José de Resende; http://lattes.cnpq.br/7949776443064259; Fernandes, Juliano José de Resende; Cabral Filho, Sérgio Lúcio Salomon; Manzano, Ricardo PereiraWith the intensification of production systems, additives such as antibiotics have been widely used in the animals' diet in order to manipulate the rumen environment, avoiding metabolic disorders and improving the digestive efficiency of nutrients. However, some antibiotics have in their composition substances also present in medicines for human use, which can make treatments less effective due to the resistance of certain microorganisms, in addition to the high potential to pollute the environment, and can be banned from the diet provided to animals. In order to replace the use of antibiotics in the diet of cattle, this study used yeast-based products that are considered natural products and have a lower acquisition cost when compared to some antibiotics. The viability of this substitution was evaluated through the digestibility of nutrients and ruminal parameters. Five steers with dairy aptitude were distributed in a 5x5 Latin square. The diets contained roughage: concentrate ratio of 35.5: 64.5, with corn silage being the roughage used, and the concentrate was composed of ground corn, soybean meal, soybean husk, mineral core, calcitic limestone, urea and common salt. . The treatments were: VM: 18mg / kg in the material (MS) of Virginiamycin (VM); CL7: 7g of yeast culture; CL14: 14g of yeast culture; LA7: 7g of autolysed yeast; LA14: 14g of autolyzed yeast. No significant difference was found (P> 0.05) for dry matter consumption with: 8.22; 8.45; 8.33; 8.51 and 8.27 kg consumed; the dry matter digestibility was 61.41%; 53.79%; 57.46%; 54.45% and 55.83%, average ruminal pH of 6.75; 6.76; 6.76; 6.78 and 6.69, for VM; CL7; CL14; LA7 and LA14, respectively. Likewise, no significant difference was found for short-chain fatty acids and ammoniacal nitrogen between treatments, thus suggesting the possibility of replacing Virginiamycin with yeast-based products.