Programa de Pós-graduação em Medicina Tropical e Saúde Pública
URI Permanente desta comunidade
Navegar
Navegando Programa de Pós-graduação em Medicina Tropical e Saúde Pública por Por Orientador "Cardoso, Divina das Dores de Paula"
Agora exibindo 1 - 2 de 2
Resultados por página
Opções de Ordenação
Item Análise proteômica de células a-549 infectadas por adenovírus espécie f sorotipo 40 (HAdV-40)(Universidade Federal de Goiás, 2015-08-06) Guissoni, Ana Carla Peixoto; Cardoso, Divina das Dores de Paula; http://lattes.cnpq.br/9770835116155857; Cardoso, Divina das Dores de Paula; Leite , José Paulo Gagliardi; Barardi , Célia Regina Monte; Fiaccadori , Fabíola Souza; Souza , Menira Borges de Lima Dias eHuman adenovirus (HAdVs) are causative agents of different clinical syndromes such as gastroenteritis, respiratory diseases, eye diseases and cystitis. Adenovirus infection can modify the cellular homeostasis through the interaction with the host cell by inducing proteins of several metabolic pathways. The resulting knowledge of this virus-cell interaction may aid the elucidation of the pathogenic mechanisms caused by adenovirus and the host response against viral infection. To study this interaction, a methodology that has been widely used is proteomics, a tool used in this study, which aimed to identify induced proteins due to viral infection. In this context, we used cells A-549 infected with human adenovirus of type F, serotype 40 (HAdV-40). Infected cells and non-infected cells were used for the osmotic lysis, which were quantified by the Bradford method and then digested with trypsin. Peptides were separated on the LC system in two dimensions. The ionization of the peptides was performed by nano-eletronspray source and through analysis of ToF-MSE system aiming the protein identification. A sum of 336 proteins were identified, 206 of them induced and 130 suppressed by the infection with HAdV-40. The main pathways affected by viral infection were: energy, cellular organization, stress response and apoptosis. It was observed alteration of cell metabolism with increase of the glycolytic pathway, β-oxidation and respiratory chain. Also, the results suggest cytoskeleton reorganization and apoptosis induction. The data can to contribute knowledge about adenovirus pathogenesis considering the proteins related to distinct metabolic pathways induced by viral infection.Item Detecção e caracterização de calicivírus em amostras fecais de crianças frequentadoras de creche em Goiânia, Goiás(Universidade Federal de Goiás, 2013-04-17) Oliveira, Denisy Marques Mendanha de; Souza, Menira Borges Lima dias e; http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4705032H5; Cardoso, Divina das Dores de Paula; http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787251Z2; Cardoso, Divina das Dores de Paula; Féres, Valéria Christina Rezende; Pereira, MaristelaThe calicivirus (CV) are important etiologic agents of acute gastroenteritis, affecting people of different ages and in various parts of the world. The present study aimed at the detection and molecular characterization of caliciviruses - norovirus (NoV) and sapovirus (SaV) - from fecal samples from children attending a daycare in Goiânia, Goiás. Were collected and analyzed 539 fecal samples 56 children from October 2009 to October 2011. The viral detection and genotyping was done by polymerase chain reaction post-transcription (RT-PCR) and by genomic sequencing using primers designed for partial regions of the viral capsid. From the 56 children participants of the study, 29 (51.8%) had at least one positive sample for CV during the study period. Positive children, 21 (37.5%) had at least one sample for NoV, 16 (28.6%) for SaV and eight (14.3%) positivity to both the virus. The CV were detected in children of both genders, and a higher positivity rate was detected among children aged 13 to 24 months, when compared to the other age groups. A significantly higher positivity for CV was observed in samples from asymptomatic children, and a defined seasonality pattern was not detected for these agents. In terms of molecular characterization for NoV the circulation of samples GII.6, GII.2, GII.1, GII.4, GI.1 and GI.7 was observed, whereas for SaV samples were detected GI.1 and GI.3. During the study period five outbreaks took place, being three of NoV, GII.6, GII.2 and GII.1, two SaV GI.1 and GI.3. Two children presented by prolonged NoV excretion, one for two months and another for 23 days. Twelve (20.4%) children had more than infection for NoV and / or SaV. This study showed circulation of CV at the daycare, a higher rate of infection in asymptomatic children, and prolonged de NoV excretion, which may have contributed to the occurrence of outbreaks and to the dissemination of these agents in such environment.