Mestrado em Ciência Animal (EVZ)
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Item Estudo comparativo entre dois critérios de classificação histológica, contagem de agnor’s e expressão de c-kit no mastocitoma canino(Universidade Federal de Goiás, 2013-09-27) Borges, Juliana Carvalho de Almeida; Moura, Veridiana Maria Brainezi Dignani de; Prado, Yandra Cássia Lobato do; Araújo, Eugênio Gonçalves de; http://lattes.cnpq.br/3919777570059928; Araújo, Eugênio Gonçalves de; Miguel, Marina Pacheco; Damasceno, Adilson DonizetiIn dogs, mastocytoma is the most common skin cancer, representing up to 21% of all skin tumors. Although the most consistent prognostic factor in diagnosis is histopathological evaluation, different classification systems for canine cutaneous mast cell tumors are used, reducing the prognostic value of histological grading. The Patnaik system, based on a three-level classification, is still the most used, despite its subjectivity. In 2011, KIUPEL and collaborators proposed a new histological classification system, sorting the tumors into two classes, low intensity and high intensity. The objective of this study was to evaluate the comparison between the methods of classification of Patnaik and Kiupel, and establish a possible relationship between the biological behavior of Kiupel method classified mastocytoma with the through the histochemical AgNOR's technique and the expression of c-KIT. Samples of canine cutaneous mastocytoma from the Laboratory of Animal Pathology, School of Veterinary and Animal Science, Federal University of Goiás were used for this purpose. In addition to highlighting the subjectivity of tumors classified as intermediate grade system by Patnaik, the analysis indicated that the Kiupel’s method brought up greater agreement among pathologists, confirming its efficiency. The proliferative index determined by AgNOR's was greater in high intensity mastocytomas. In general, the expression of c-KIT corroborated the histological classification. The results showed that the new Kiupel classification system, is more efficient for the histological diagnosis and prognosis of canine mastocytoma when coupled with immunohistochemical and histochemical study.Item Atividade citotóxica do extrato etanólico da casca de pequi (Caryocar brasiliense) em células de osteossarcoma canino in vitro(Universidade Federal de Goiás, 2016-03-04) Braga, Karla Márcia da Silva; Bianchi Filho, Cesario; http://lattes.cnpq.br/1927380763523677; Araújo, Eugênio Gonçalves de; http://lattes.cnpq.br/3919777570059928; Araújo, Eugênio Gonçalves de; Damasceno, Adilson Donizeti; Prado, Yandra Cássia Lobato doOsteosarcoma is a mesenchymal high mortality tumor of dogs and men. Cytotoxicity assays employing tropical plants have been performed in order to identify possible active compounds able to inhibit neoplastic cells proliferation. The fruits from the Cerrado, Center-west Brazil’s diversity-rich, predominant vegetation, are well-known by the general public but very little is known about their pharmacological properties. Thus, the aim of this study was to verify the cytotoxic activity of the ethanoic extractItem Análise química de extratos de Caryocar brasiliense com potencial antioxidante(Universidade Federal de Goiás, 2019-03-01) Ferreira, Claudiane Marques; Cruz, Vanessa de Sousa; http://lattes.cnpq.br/8788967925940484; Araújo, Eugênio Gonçalves de; http://lattes.cnpq.br/3919777570059928; Araújo, Eugênio Gonçalves de; Terezan, Ana Paula; Araújo, Luciana Batalha Miranda deThe pequi (Caryocar brasiliense) is a typical Brazilian fruit that stands out for its high nutritional value, besides its sensorial attributes like flavor, aroma and color. The fruit peel is a byproduct of industrial processing and has potential for explotation due to the presence of phenolic compounds that act in the animal body as antioxidants, capable of preventing or preventing the action of free radicals that cause degenerative diseases. The objective of this work was to analyze the presence of phenolic compounds and their antioxidant activities through chemical analyzes of crude ethanolic extract and its hexane, dichloromethane, ethyl acetate and aqueous fractions of Caryocar brasiliense bark. The identification of phenolic compounds was performed by high performance liquid chromatography coupled to high resolution mass spectrometry. Phenol content was analyzed by determination of the total phenolic profile by the Folin-Ciocalteu method and the antioxidant capacity was analyzed by the in vitro antioxidant activity method by the DPPH assay. A total of fifteen phenolic compounds were identified by comparison with the standards: gallic, ellagic, protocatechic, gentilic, vanillic, caffeic, ferulic and p-coumaric acids. In addition to catechin, epicatechin, luteolin, kaempferol, quercetin, rutin and naringerin. The dichloromethane, ethyl acetate and aqueous fractions showed thirteen of the fifteen phenolic compounds, the crude extract twelve and the hexane ten. The total phenol profile ranged from 0.6 to 48 mg / 100 mL and the in vitro antioxidant activity showed high values ranging from 26% to 99.4%. The results confirm that the crude extract of the bark of the Caryocar brasiliense fruit and its hexane, dichloromethane, ethyl acetate and aqueous fractions have phenolic compounds and antioxidant activity.Item Propriedades citotóxicas da Beta-Lapachona em células de osteossarcoma in vitro(Universidade Federal de Goiás, 2017-03-09) Gabriel, Gabriela Hadler; Bianchi Filho, Cesário; http://lattes.cnpq.br/1927380763523677; Araújo, Eugênio Gonçalves de; http://lattes.cnpq.br/3919777570059928; Araújo, Eugênio Gonçalves de; http://lattes.cnpq.br/3919777570059928; Oliveira, Cairo Henrique Sousa de; Cruz, Vanessa de SousaOsteosarcoma is the main primary bone tumor, with unfavorable prognosis, high mortality and high incidence of metastases. The treatment of choice is the removal of the tumor associated with combined chemotherapy, whose adverse effects allude to the increasing need to develop new drugs. The plants constitute a large natural reserve of compounds that have medicinal and therapeutic properties, such as lapachol and its derivative, β-lapachone. The aim of this study was to evaluate the cytotoxic effects of β-lapachone in osteosarcoma cells cultured in vitro. Cells were cultured and treated with β-lapachone at different concentrations and times of exposure. Tripan blue exclusion, tetrazolium reduction and cell survival assay methods were performed to evaluate the effects of the compound on the cells. Cells treated with 0,1μM β-lapachone showed lower initial cytotoxicity in the 24h time, whereas those submitted to 1,0μM showed less viability after 72h of treatment. Cytotoxicity increased as the concentration and time of exposure increased. The lowest IC50 (0,148μM) was observed in treated cells for 72h. Cell growth after treatment was lower in the 1.0μl group after 72h and the highest cell growth was observed under a concentration of 0.1μl after 24h. There was no difference between groups for cell proliferation after treatment, and the cell survival fraction was lower after 72h of exposure. It was concluded that β-lapachone has cytotoxic effects on osteosarcoma cells cultured in vitro.Item Ação citotóxica e antioxidante da beta lapachona em células endoteliais(Universidade Federal de Goiás, 2017-12-18) Gonçalves, Patricia de Almeida Machado; Cruz, Vanessa de Sousa; http://lattes.cnpq.br/8788967925940484; Pereira, Kleber Fernando; http://lattes.cnpq.br/4779531102983799; Araújo, Eugênio Gonçalves de; http://lattes.cnpq.br/3919777570059928; Araújo, Eugênio Gonçalves de; Santin, Ana Paula Iglesias; Porto, Regiani Nascimento GagnoOxidative stress is the overproduction of reactive oxygen species that cause cell damage and even the death. It is a mechanism related to ischemia and reperfusion, a process that affects several organs and is involved in neurodegenerative diseases, cardiac and/or renal insufficiencies, hepatic dysfunction, among others. The discovery of new antioxidant substances derived from natural sources, is important for the prevention and treatment of these diseases. Beta lapachone is a substance with antioxidant action obtained from sawdust of Ipe wood, a symbolic tree from the Brazilian cerrado. This study aimed to analyze the cytotoxic and antioxidant action of beta lapachone in endothelial cells, in vitro. EA.hy 926 lineage cells were subcultured, treated with different concentrations of beta lapachone and subjected to ischemia and reperfusion. Cytotoxicity and antioxidant action were assessed through the cellular viability analysis by the tetrazolium reduction method. The averages were verified by the Tukey test (5% of significance) and by variance analysis. There was a dose-dependent progression of cytotoxicity in the non-ischemia/reperfusion (control) group and cell viability increase in the ischemia/reperfusion group. However, there was no statistical difference between the concentrations and between groups. Considering the settings of this experiment, Beta lapachone did not present cytotoxic or antioxidant action on endothelial cells.Item Efeitos do plasma rico em plaquetas na expressão de metaloproteinases MMP-2 e MMP-9 no líquido sinovial de equinos com sinovite induzida(Universidade Federal de Goiás, 2019-06-26) Peixoto, Andréia da Costa; Brandstetter, Luciana Ramos Gaston; http://lattes.cnpq.br/0184140058949751; Moura, Veridiana Maria Brianezi Dignani de; http://lattes.cnpq.br/8773201078957745; Araújo, Eugênio Gonçalves de; http://lattes.cnpq.br/3919777570059928; Moraes, Júlia de Miranda; Araújo, Eugênio Gonçalves de; Noronha Filho, Antônio Dionísio FeitosaThe equine musculoskeletal system suffers contant overload, what increases its predispositon to injuries, particularly the development of joint diseases. Conventional anti-inflammatory treatments have both temporary and symptomatic effects, as well as unwanted side effects. Platelet Rich Plasma (PRP) has been studied as a regenerative cellular therapy for joint diseases as it aims the modulation of extracellular matrix degradation and regeneration (MEC) factors. Important degradation factors are the metalloproteinases (MMPs), especially MMP-2 (Matrix metalloproteinases type 2) and MMP-9 (Matrix metalloproteinases type 9) in equines. Therefore the aim of this dissertation is to review the elements involved in the arthropathies, and investigate the intra-articular effects of PRP on the MMP-2 and MMP- 9expression. Twelve healthy horses were divided into groups: Control and Treatment (CG and GT). Induction of synovitis was performed in all horses by means of lipopolysaccharide (LPS) injection into the left radiocarpal joint. GT was treated with a single intrasinovial PRP injection. PRP was obtained by double centrifugation method. The expression of MMP-2 and MMP-9 in the synovial fluid was determined by ELISA test. Density, pH, glucose, protein, red blood cells and nucleated cells were also determined. These analysis were done prior to and after induction of synovitis and six, 24 and 48 hours after PRP injection. Regarding MMPs expression, there was no difference between groups (p> 0.05), as well as in the evaluation of glucose, red blood cells and nucleated cells. However, density, pH and proteins were different in the comparison between the two groups at six hours post PRP treatment (p = 0.0057; p = 0.0233; p = 0.0152, respectively). In the experimental model of induced synovitis in horses, PRP seems to have an influence on MMP-2 levels, but not on MMP-9, although it was not statistically observed. In addition, it also influenced the physico-chemical and cytological aspects of LS.Item Detecção molecular de herpesvírus bovino tipo-1 e herpesvírus bovino tipo-5 em amostras de encéfalos bovinos incluídos em parafina(Universidade Federal de Goiás, 2014-09-04) Silva, Danilo Rezende e; Linhares, Guido Fontgalland Coelho; Cunha, Paulo Henrique Jorge da; Araújo, Eugênio Gonçalves de; http://lattes.cnpq.br/3919777570059928; Araújo, Eugênio Gonçalves de; Miguel, Marina Pacheco; Prado, Yandra Cássia Lobato doIdentification of herpesvirus in specimes fixed in formalin and paraffin embedded has been used in experimental analysis. Several molecular techniques have been used in research and in diagnosing of herpesviral meningoencephalitis. 80 cases of routine in veterinary pathology were reviewed on the Department of Animal Pathology of Veterinary and Science Animal School Medicine of Federal University of Goias, Goiania, GO (EVZ/UFG) performed between January 2007 to December 2012. Were used 18 cases with a total of 44 paraffin blocks (Group I - 14 blocks, Group II - 17 blocks and Group III - 13 blocks), which were grouped according to histopathological diagnosis, in Group I - samples without histological changes and no clinical history of neurological disease, Group II - samples with nonspecific encephalitis, Group III - samples with rabies encephalitis. Were extracted DNA fragments, from cuts blocks, using a commercial extraction kit of formalin and paraffin embedded tissue. Afterwards the technique standardization of conventional PCR for bovine herpesvirus-1 and bovine herpersvírus type-5 was performed. Were positive for BoHV-5 28% (5/18) of cases, with 11% (2/18) of the group with nonspecific encephalitis and 17% (3/18) of the group with encephalitis and positive for the rabies virus. In the test for checking of the bands for the BoHV-1, all samples, 100% (18/18) were negative. These results demonstrate that the conventional PCR technique is helpful in aiding the definitive diagnosis of encephalitis bovine herpesvirus type-5.Item Citotoxicidade de extratos das folhas de gabirobeira (Campomanesia adamantium) em células de osteossarcoma in vitro(Universidade Federal de Goiás, 2018-06-29) Silva, Nara Cristina; Cruz, Vanessa de Sousa; http://lattes.cnpq.br/8788967925940484; Damasceno, Adilson Donizeti; http://lattes.cnpq.br/3900110295277130; Araújo, Eugênio Gonçalves de; http://lattes.cnpq.br/3919777570059928; Pôrto, Regiani Nascimento Gagno; Santin, Ana Paula Nascimento Iglesias; Araújo, Eugênio Gonçalves deOsteosarcoma is a highly aggressive malignant bone tumor that has an unfavorable prognosis. The treatment of choice is surgical intervention associated with systemic chemotherapy. However, the chemotherapeutic agents currently employed have several adverse effects on patients that limit their use. Thus, research aimed at new alternatives to the treatment of this cancer is of paramount importance. This study aimed to evaluate, in vitro, the cytotoxic potential of extracts from leaves of gabirobeira in osteosarcoma cells. From the leaves of this plant, the crude ethanolic extract and ethyl acetate were extracted. The cells were cultured and treated with both extracts at different concentrations and at predetermined exposure times. Cell cytotoxicity was analyzed by the Tripan blue exclusion assay and the IC50 calculated by the tetrazolium reduction method. The crude extract presented higher cytotoxicity compared to acetate, and the best result was in the 1.0μL/mL group treated for 48 hours. In addition, the IC50 value for G48 is within the range suggested as the best cytotoxicity. Finally, extracts of the leaves of the gabirobeira presented cytotoxic action in the cells of in vitro osteosarcoma.