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Item Utilização do óleo obtido de resíduos agroindustriais de goiaba (Psidium guajava L.) na nutrição de poedeiras comerciais(Universidade Federal de Goiás, 2014-10-31) Barbosa, Nathalia Pedroso; Stringhini, Jose Henrique; http://lattes.cnpq.br/8505634095383289; Mello, Heloísa Helena de Carvalho; http://lattes.cnpq.br/5510965166352073; Conceição, Edemilson Cardoso da; http://lattes.cnpq.br/7193007113950510; Conceição, Edemilson Cardoso da; Bara, Maria Teresa Freitas; Café, Marcos BarcellosPsidium guajava L. is a plant originated in Mexico, belonging to mytarceae family. The guava fruit is growth in economic value for the production of derivatives in the Brazilian agribusiness. However the use of guava pulp generates waste discarded in the environment, without the correct use, ie an environmental liability. The guava residue consists of pulp, peel, but mainly seeds. Yet is not described in the literature depth studies on its chemical composition or a suitable waste recovery. Thus, this study aimed to characterize the dust of organic residues of guava (donated by Food Predilecta ® - Matão, São Paulo), followed by obtaining the ethanol extract of the percolation method. The ethanol extract was characterized as the fatty acid profile, density and acid number and came to be known as: Powder oil waste processing Guava (OWG) from the OWG obtained the volatile fraction by hydrodistillation and characterization was carried out by gas chromatography coupled to mass spectrometry (GC / MS), followed by characterization of β-caryophyllene marker for high performance liquid chromatography (HPLC) and the identification of ORG constituents by GC / MS. We evaluated the antioxidant potential by free radical trapping methods 2,2-diphenyl-1- picryl hydrazyl (DPPH) and the capture of ferrous sulfate (FRAP). And finally, evaluated the implementation of the OWG, in laying hens Bovans White to verify the performance, posture, egg quality, digestibility and chemical composition of eggs and lipid oxidation of gems. The analysis of the volatile fraction of the OWG by GC / MS resulted in the identification of 25 compounds having as the major, the β-caryophyllene with 21.15%. Thus, an analytical HPLC method was revalidated in accordance with the criteria of Resolution 899/03 of ANVISA for quantification of β-caryophyllene (26.26%) in the OWG, to then be used for monitoring and characterization of OWG. Moreover, GC / MS, were identified in OWG, vitamin E and phytosterols such as α- tocopherol and the β-tocopherol, β-sitosterol and campesterol. OWG showed potential antioxidant activity, with EC50 31.24 mg / mL for the capture of DPPH and a content of 3,67.10-6 mM of ferrous sulfate per gram of capture OWG. Performance reviews and posture did not differ between treatments. The eggs of the birds supplemented with ORG and BHT had favored characteristics in relation to egg weight, yolk weight, White egg weight, suggesting the replacement of BHT, a commercial antioxidant with potential carcinogen by OWG. In supplementation in the diet digestibility analysis of OWG and BHT favored the absorption of protein and feed lipids, suggesting that because the oil antioxidant potential in preserving the laying of the power of nutritional constituents as well as to preserve the moisture content, low value caloric albumen and the lipid content in the yolks. The results showed an egg production with superior quality compared to conventional production. The work generates natural antioxidant additive application alternatives that results in improved quality, productivity and reduction in production costs. Moreover favors the export in view of the new requirements of importing countries products of Brazilian poultry.Item Extrato líquido padronizado em capsantina a partir de Capsicum chinense Jacq. (Variedade Biquinho) e suas aplicações no desenvolvimento de um produto cosmético anti-aging(Universidade Federal de Goiás, 2018-12-13) Brito, Lucélia de Sousa; Conceição, Edemilson Cardoso da; http://lattes.cnpq.br/7193007113950510; Conceição, Edemilson Cardoso da; Borges, Leonardo Luiz; Silva, Luis Antônio DantasIntroduction: The pepper Capsicum chinense Jacq., popularly known as "biquinho pepper", has been outstanding in Brazil for presenting low pungency and a strong aroma. Its fruits are composed of several secondary metabolites, especially carotenoids, with important antioxidant properties. Objective: Develop a cosmetic product from the liquid extract of Capsicum chinense Jacq. standardized on capsanthin, evaluate its anti-aging efficacy in vivo and antioxidant activity. Methodology: The fruits of the "biquinho pepper" were sanitized, weighed, dehydrated, ground in a knife mill and hammers and stored at room temperature. The physico-chemical characterization of the pulverized vegetable raw material was carried out using the methods of microscopic analysis of the powder, determination of the volatile content, total ash, granulometric distribution and swelling index. Afterwards, the raw material was applied in the preparation of the liquid extract, using the techniques of maceration, percolation and concentration by forced ventilation, using the ethyl alcohol 96o GL as solvent extractor. The liquid extract was then characterized for solids content, pH, density and viscosity. Subsequently, total phenol concentration was determined by the technique of complexation of the phenolic compounds with ferric chloride solution, and also, the assay of total flavonoids and carotenoids by spectrophotometric methods. In addition, an analytical method by HPLC was validated for the quantification of the capsanthin in the concentrated liquid extract. Two cosmetics formulations were developed to perform comparative efficacy tests, a phytocosmistry developed from the liquid extract standardized on capsanthin, and another placebo formulation composed only of the galenic base of the product, without the addition of the extract. The cosmetics formulations were then evaluated for their accelerated stabilities and clinical efficacy anti-aging in vivo, evaluated in humans. The antioxidant activity was determined for the vegetable raw material, standardized liquid extract in capsanthin and the cosmetic formulation, using the DPPH radical capture, β-carotene / linoleic acid capture and ABTS radical capture assays. Results and discussion: For the physical-chemical characterization of the pulverized vegetable raw material, the microscopic analysis of the powder demonstrated the intimate nature of the sample as well as its purity, presented a volatile content of 4.62% (m / m), total ash content of 6.341%, the determination of the granulometric distribution classified the sample as coarse powder and the intumescence index was 1.27 ml ± 0.12 using alcohol 96o GL as an intumescent agent. In the physical-chemical characterization of the liquid extract the solids content was 19.16%, pH 3.7 ± 0.06, density 1.040 ± 0.0014 g / mL and viscosity of 3.22 mPas. The total phenolics, flavonoids and carotenoids contents for the raw material and concentrated liquid extract samples were respectively: 18 mg / 100 g and 168 mg / 100 g phenols, 10 mg / 100 g and 90.7 mg / 100 g flavonoids, 10mg / 100g and 83,70 mg / 100g of carotenoids. The validation of the analytical method by HPLC used in the quantification of capsanthin proved to be selective, linear (in the range of 0.48 to 7.2 μg / ml for the capsanthin standard and 26.93 to 404 mg / ml for the liquid extract), accurate, exact and robust. The content of capsanthin found in the liquid extract of C. chinense Jacq. was 1.126% (m / m). Both the standardized liquid extract and the cosmetic formulations were stable against accelerated stability tests, with results of organoleptic and physico-chemical characteristics within the parameters established for the tests. The analysis of the clinical anti-aging efficacy in vivo for cosmetic formulations has demonstrated the potentiation of efficacy for the cosmetic formulation consisting of the standardized liquid extract of Capsicum chinense Jacq., Compared with the placebo cosmetic, showing improvements in wrinkles, expression lines and sagging skin. The antioxidant activity in vitro for the vegetable raw material, standardized liquid extract in capsanthin and cosmetic formulation were respectively: 194.6μg / ml, 73.7μg / ml and 100μg / ml for capturing the DPPH radical relative to the IC50, 33.03%, 42.08% and 19.35% for inhibition of β-carotene oxidation, 22.87 μM Trolox / g, 51.81 μM Trolox / g, 900 μM Trolox / g for the ABTS radical capture. The values obtained for evaluations of antioxidant activities in vitro indicated that all the samples showed to be effective by antioxidant action, although the results have been lower if compared to their respective positive controls. Conclusions: The cosmetic product developed showed antioxidant activity and intended anti-aging efficacy, proving of potential use of the standardized liquid extract of biquinho pepper (Capsicum chinense Jacq.) in technological and innovative phytocosmetic formulations.Item Desenvolvimento tecnológico de extratos vegetais padronizados a partir da Lafoensia pacari A. St.-Hill (Lythraceae)(Universidade Federal de Goiás, 2013-02-28) Cardoso, Natasha Queiroz; Conceição, Edemilson Cardoso da; http://lattes.cnpq.br/7193007113950510Lafoensia pacari A. St.-Hill (Lythraceae) popularly known as dedaleiro, didal, or mad-mangabeira pacari, is used by the Brazilian population as a febrifuge, tonic and healing. Studies have shown the antioxidant, anti-inflammatory, and antinoceptiva antiedematogenic extracts from the stem bark, ellagic acid being one of those responsible for the same. This study aimed to obtain dry extract standardized technology from the stem bark of L. pacari. The drug was tested for various quality control tests, according to the Brazilian Pharmacopoeia 5th edition, which ensured the characterization of plant drug and subsequently obtaining the liquid and dry extracts standardized. The extraction method was chosen slow percolation with 80% hydroalcoholic solution as a solvent and velocity of 7 drops per min .. The liquid extract standardized had a density of 0.986 g / mL solids content of 12.49% (w / w), pH 3.53 and an alcohol content of 44.80% (v / v). The content of ellagic acid found in liquid extract was 3.84% (m / m). The spray drying / atomization was performed by experimental design of univariate and then by planning a fractional 5-2 with 2 replicates. During the process the adjuvants were used maltodextrin and aerosil, demonstrating that the optimal conditions found maltodextrin provides greater protection and ellagic acid concentration in the extract.Item Oleorresina de Copaifera spp.: caracterização, verificação da atividade antimicrobiana in vitro e avaliação preliminar de uma formulação em vacas leiteiras com mastite(Universidade Federal de Goiás, 2014-05-07) Faria, Maria Juíva Marques de; Braga , Carla Afonso da Silva Bitencourt; Conceição, Edemilson Cardoso da; http://lattes.cnpq.br/7193007113950510; Conceição, Edemilson Cardoso da; Mourão, Rosa Helena Veras; Borges, Leonardo LuizBovine mastitis is an inflammatory/infectious process of the mammary gland, its etiology is complex and multivariate. The most common mastitis has bacterial origin. Because of the high cost to treat this disease, and the bacterial resistance to antibiotics, beyond the losses due to the milk discard or the presence of drug residues in milk, studies are necessary to find out alternative methods to circumvent these problematic. The medicinal plants with antimicrobial activity have been making room in veterinary medicine with the objective to reduce the impact and the use of drugs indiscriminately, as well as maintain the integrity of the animal and control the dissemination of resistant bacterial lineage. Therefore, the aim of this work was characterize the oleoresin Copaifera spp., verify the antimicrobial activity in vitro of oleoresin and essential oil of Copaifera spp. in combating against aerobic and facultative isolated bacteria from cows’ milk with subclinical grade III mastitis diagnostic and evaluate preliminarily, in vivo, phytotherapic formulation composed of Copaifera spp. The tests for characterization of the oleoresin, confirmed its authenticity and quality, considered appropriate for use. The analyzes performed by gas chromatography coupled to mass spectrometry (GC/MS) and electrospray ionization Fourier transformed íonic cyclotron resonance mass spectrometry (ESI FT-ICR MS) identified sesquiterpenes and diterpenes present in the essential oil and oleoresin of Copaifera spp., respectively. The technique of High Performance Liquid Chromatography (HPLC) for sesquiterpenes, especially for β-caryophyllene, can be used in quality control of essential oil and oleoresin of Copaifera spp., as well as in phytotherapic formulation using oleoresin of Copaifera spp. The oleoresin showed better antimicrobial activity than the essential oil of Copaifera spp. (ρ<0,0001) in combating the 55 isolated bacteria. The antibiogram test of the three phytotherapic formulations demonstrated that the oleoresin had antimicrobial activity against the isolated microorganisms. The preliminary evaluation, in vivo, of a phytotherapic formulation containing 20% oleoresin of copaiba was neither effective nor safe, because it intensified the inflammatory process, requiring further tests, such as cytotoxicity and irritability of the raw material plant and of the vehicle used. Key words: Bovine Mastitis. Oleoresin. Essential Oil. Copaifera spp. Minimum Inhibitory Concentration. ESI FT-ICR MS.Item Extrato padronizado de Rosmarinus officinalis L. (Lamiaceae) na supressão da brusone foliar em arroz(Universidade Federal de Goiás, 2015-11-27) Garcia, Mythali Lima; Filippi, Marta Cristina Corsi; http://lattes.cnpq.br/0029536556461484; Conceição, Edemilson Cardoso da; http://lattes.cnpq.br/7193007113950510; Conceição, Edemilson Cardoso da; Gonçalves, Fábio José; Barra, Maria Teresa FreitasThe blast caused by the fungus Magnaporthe oryzae, is a disease affecting rice significantly throughout the world. Seeking its control, fungicides have been used indiscriminately. With the need to develop alternative control methods, the uses of plant extracts have been a promising strategy for the control of phytopathogens. The objective of this study was to develop and characterize the liquid extract of Rosmarinus officinalis standardized in rosmarinic acid and evaluate its potential as resistance inducer and suppressor of leaf blast on rice crops. The standardization was initiated by characterization of plant material followed by obtaining the liquid extract by percolation method. The liquid extract was characterized as pH, viscosity, density, solids content and chromatographic profile for Thin Layer Chromatography. A method by high-performance liquid chromatography to quantify the rosmarinic acid in the liquid extract was validated. In Embrapa Rice and beans, rice seeds were sown and three concentrations of R. officinalis extract and standard of rosmarinic acid were sprayed and monitored in plants to test their respective capabilities suppression of leaf blast and inducing resistance in rice from increase enzyme activity of chitinase, β-1,3 glucanase, peroxidase, lipoxygenase, phenylalanine ammonia lyase, of phenolic compounds and salicylic acid hormone, before and after challenge with M. oryzae. All treatments reduced the severity of leaf blast in rice in more than 80%. In the absence of the pathogen from four hours, all treatments increased the expression of enzymes related to the defense of the plant, the phenolic compounds and salicylic acid hormone, except for the peroxidase. In the presence of M. oryzae, the enzymatic activity of GLU, CHI, POX, LOX, PAL, phenolic compounds and salicylic acid content increased significantly. The standardized rosemary extract in rosmarinic acid has proven to be a powerful alternative method for controlling the blast in rice and may be associated with integrated management techniques.Item Extrato padronizado de Ruta graveolens L.: avaliação de seu potencial no controle da brusone em arroz(Universidade Federal de Goiás, 2013-12-18) Reis, Karinna Bannach; Filippi, Marta Cristina Corsi de; http://lattes.cnpq.br/0029536556461484; Conceição, Edemilson Cardoso da; http://lattes.cnpq.br/7193007113950510; Bresolin, Tania Mari Bellé; http://lattes.cnpq.br/2248201275641475; Fonseca, Maria Jose Vieira; http://lattes.cnpq.br/9252516331552117Ruta graveolens has been successfully applied for many human diseases treatment and promises a well succeed alternative for plant diseases control because it has also phytoalexins in its composition. The aim of this study was to standardise the R. graveolens liquid extract and evaluate its potential for controlling rice (Oryza sativa) leaf blast (Magnaporthe oryzae). The drug has been characterized, the liquid extract obtained and the methodology for quantifying the standards components, furanocoumarins psoralen and bergapten, validated by high performance liquide chromatography. The components of essential oil obtained from standardized liquid extract were characterized by gas chromatography-mass spectrometry. In a completely randomized design, conducted in artificial hydrophobic surface with three replications and eleven treatments composed of M. oryzae conidial suspension (105 con.ml-1) mixed with R. graveolens standardized extract (0.01 to 0.10 g.mL-1), or furocoumarins psoralen (0.18 to 1.82 μg. mL-1), or bergapten (0.29 to 2.91 μg. mL-1), or water (control). It was evaluated the inhibition of conidial germination and appressorium formation and the median lethal dose (LD50). A second assay, in a completely randomized design in three replications was conducted in greenhouse conditions. It was composed of 21 days old rice plants, which were sprayed with a mixture containing M. oryzae conidial suspension (3x105 con.ml-1) and R. graveolens extract, without dilution, or furocoumarins psoralen (18.26 μg. mL-1), or bergapten (29.14 μg. mL-1), or water (control). Nine days after inoculation, leaf blast severity was scored with a diagrammatic scale, the data were statistically analyzed and means compared. The standardized plant extract inhibited M. oryzae conidial germination (LD50=0.237mg) and appressorium formation (LD50=0.121 mg) up to 100% and reduced 80.84% of leaf blast. By fluorescence microscopy it was possible to observe that standardized plant extract did not damage M. oryzae cell wall and plasma membrane, indicating another type of interaction to inhibit conidia development. Isolated standards furanocoumarins psoralen and bergapten did not inhibit conidial germination and appressorium formation and reduce leaf blast severity proportionally, suggesting that synergistic interactions between extract and essential oil components were responsible for the success of R. graveolens in suppressing rice disease, making it an alternative to compose rice blast management.Item Aproveitamento da casca e polpa de Baru (Dipteryx alata Vogel) no desenvolvimento de um protótipo de suplemento alimentar, enriquecido com selênio(Universidade Federal de Goiás, 2019-06-28) Santos, Cláudia Maria Barbosa; Santiago, Raquel de Andrade Cardoso; http://lattes.cnpq.br/0424807117498265; Conceição, Edemilson Cardoso da; http://lattes.cnpq.br/7193007113950510; Santiago, Raquel de Andrade Cardoso; Nóbrega, Andréa Bezerra da; Morais, Carla Cristina deIntroduction. Baru is a plant typical of the Cerrado Biome, whose nutritional potential is little explored, especially in the case of bark and pulp. It is known that baru has a fibrous pulp, and despite the benefits it can provide such as, reduced food consumption by increasing satiety power and consequent reduction in waist hip ratio, control of arterial hypertension and improvement of intestinal functioning , 68% of the population has less fiber intake than recommended. For this reason your supplementation may be beneficial. In addition to fiber, another important nutrient for health is selenium, given its association with changes or malfunctioning of the digestive tract. Objective. Develop a prototype food supplement in sachets, from bark and pulp of baru, that meets the daily nutritional needs of fiber and selenium. Methods. The raw material was obtained and characterized. The microbiological quality, centesimal composition, evaluation of the accelerated stability study of bark pulp powder and baru pulp and the prototype of the supplement were evaluated. Results and discussion. The raw material was adequate for the quality parameters. With the addition of excipients the prototype remained stable, with respect to the microbiological profile, humidity, fibers, selenium and other parameters. Two sachets with 30 g of supplement provides an energy value of 67.40 kcal, 1.56 g of protein, 0.82 g of lipids, 13.44 g of carbohydrates, 9.42 g of fibers, 200.32 g of selenium, 24.20 mg of calcium, 144.90 μg of copper, 0.98 mg of iron, 0.018 mg of phosphorus, 9.86 mg of magnesium, 0.283 mg of manganese, 211.468 mg of potassium, 6.591 mg of sodium and 0.225 mg of zinc. The labeling of the prototype of the supplement may be attributed as: source of fibers, source and / or selenium-rich, source of copper and low sodium content. Conclusion. The elaborated formulation was able to meet the recommendations for food supplement in relation to dietary fiber and selenium, for the population group above 19 years of age, can be used for other food purposes and also contribute to the use of parts of the fruit that are discarded.Item Obtenção de pellets a partir dos extratos líquidos padronizados de Brosimum gaudichaudii Trécul (Moraceae) ou Lafoensia pacari A. St.-Hil. (Lythraceae)(Universidade Federal de Goiás, 2014-07-11) Silva Filho, Omar Paulino; Conceição, Edemilson Cardoso da; http://lattes.cnpq.br/7193007113950510; Conceição, Edemilson Cardoso da; http://lattes.cnpq.br/7193007113950510; Veiga Júnior, Valdir Florêncio da; Borges, Leonardo LuizPellets are spherical solid oral dosage forms that can be used in the development of new pharmaceutical products with standardized plant extracts. Brosimum gaudichaudii Trécul (Moraceae) and Lafoensia pacari A. St.-Hil. (Lythraceae) are typical species from the Cerrado biome. The extract obtained from the roots of B. gaudichaudii is traditionally used in the treatment of vitiligo, its effectiveness is due to the presence of the chemical markers psoralen and bergapten. As for L. pacari, the barks of the branches and trunk are traditionally used in the treatment of gastric ulcers, highly valued for there anti-inflammatory and wound healing because of there ellagic acid content. For each of these species optimal extractive methods exist to obtain standardized liquid extracts. However there are no studies that propose the development of solid oral dosage forms from them. Thus, the present study is aimed at obtaining pellets from the standardized liquid extract of the B. gaudichaudii roots, furthermore to determine the efficiency of a photoprotector coating on the stability of psoralen and bergapten and also obtaining pellets with standardized liquid extract of the L. pacari barks from the branches and trunk. The B. gaudichaudii roots were collected in the city of Jussara, Goiás, and the L. pacari barks in Niquelândia, Goiás. Separately, the raw materials were cleaned, dried in an oven with forced air circulation, then subsequently milled for the extractive process. After the standard liquid extract of B. gaudichaudii had been obtained, six formulas were developed at bench scale. Among these, one stood out by the homogeneity of the lot and the sphericity of its pellets. This formula had been selected to be sacaled-up and it`s composition had been formed by 49.5% (w/w) of microcrystalline cellulose (MCC), 1% (w/w) of hydroxypropylmethyl cellulose (HPMC) and 49.5% (w/w) of that extract. The pellets obtained from the scaled formula were divided into two portions, one of them had been coated with a photoprotective layer and both were subjected to a photostability test. The degradation of the markers had been evident only in the uncoated pellets. The reduction of 1.87% (w/w) in the content of psoralen and 8.1% (w/w) in bergapten content had been observed after 3 J/cm2 exposure to UVB radiation. After exposing to 30 J/cm2 of UVA, there had been a reduction of 24.1% (w/w) of psoralen and 28.48% (w/w) of bergapten. Therefore, the application of the photoprotective coating had been an effective alternative and ensured the stability of the chemical markers after the test. With respect to the liquid extract of L. pacari, 13 formulas were obtained among which only two formed pellets whose batch homogeneity had been greater than 70%. Among them, only one had been chosen to obtain the first scaled-up model. With the production of the scaled-up batch, it had been observed that formulas prepared with a lower proportion of standardized liquid extract of L. pacari had enabled us to obtaing more homogeneous, spherical and a smoother pellets. Based on these results and due to the innovative character of the studies, this work can be used as a model for future trials designed to obtain pellets with standardized liquid extract of B. gaudichaudii or L. pacari.Item Obtenção de extratos padronizados a partir das cascas de Stryphnodendron adstringens (Mart.) Coville (Fabaceae) e avaliações biológicas in vitro(Universidade Federal de Goiás, 2014-03-28) Sousa, Jordana Nunes de; Conceição, Edemilson Cardoso da; http://lattes.cnpq.br/7193007113950510; Conceição, Edemilson Cardoso da; Silva Júnior, José Otávio Carréra; Paula, Joelma Abadia Marciana deThe present work aimed to obtain extracts from the barks of Stryphnodendron adstringens (Mart) Coville (Fabaceae) and evaluating of biological activities in vitro, from them. Chapter 1 using pharmacopoeial and not pharmacopoeial methods; barks, powder and liquid extract of S. adstringens were characterized and then a HPLC method was developed and validated to idenfication of epigallocatechin gallate (EGCG) at extracts. In Chapter 2, described the cytotoxic activity of the dry extract of S. adstringens, obtained by atomization, and the EGCG standard against L929 fibroblast cells by the method of neutral red. EGCG at 200 µg/mL reduced, p ≤ 0.05, the cell viability of L929 fibroblasts to 47.70%, such as the dried extract of S. adstringens at 225 mg/mL. Chapter 3 presented the obtainment of dry extracts of S. adstringens by atomization. The influence of the proportion of adjuvant drying, the drying air temperature and flow rate of the drying air on total phenols, EGCG, tannins, total antioxidant activity and efficiency of the drying process were evaluated, the significant results (p≤0.05) were obtained by ANOVA and represented by response surface methodology. The increase in the adjuvant drying was not significantly favorable to efficiency of the drying process. Increasing the temperature of the drying air had a quadratic behavior negative to total phenols and positive to total tannins. The increasing of flow air drying showed linear and quadratic behavior. negative to total antioxidant activity. The efficiency of the drying process was not influenced by any variable. The dried extracts were analyzed for their content of volatile, water activity and the morphologies of the particles were observed by scanning electron microscopy. In Chapter 4 we describe the optimization of extraction of total phenols, tannins and epigallocatechin gallate powders of barks of S. adstringens ultrasonic using Box- planning Benhken 33. The use and validation of the desirability global statistical showed that the proportion of ratio drug solvent 4mg/mL, time extraction 30 min and the 65 % (v/v) hydroalcoholic solution are ideal to simultaneous extraction of these metabolites. The results indicate that the extracts were obtained and standardized quality, and have potential to be investigated their biological and/or insertion activities in herbal formulations