Programa de Pós-graduação em Biologia da Relação Parasito-Hospedeiro
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Navegando Programa de Pós-graduação em Biologia da Relação Parasito-Hospedeiro por Por Orientador "Fernandes, Éverton Kort Kamp"
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Item Termotolerância de Metarhizium spp. e efeito de sua formulação sobre a virulência para Rhipicephalus microplus expostos à condições estressantes de temperatura e umidade(Universidade Federal de Goiás, 2015-04-01) Muniz, Elen Regozino; Angelo, Isabele da Costa; Fernandes, Éverton Kort Kamp; http://lattes.cnpq.br/2135541732341157; Fernandes, Éverton Kort Kamp; Arruda, Walquíria; Silva, Andréa Caetano daRhipicephalus microplus, known as tick of the cattle, cause great economic losses each year in Brazil. Entomopathogenic fungi stand out as an alternative to the exclusive control and indiscriminate use of the chemical. However, ambient temperature above the threshold considered optimal for these organisms can interfere with your biology, and compromise their development and effectiveness. In the present study evaluated biological parameters seven strains of Metarhizium spp. (IP1, IP34, IP46, IP60, IP119, IP125 and IP146) originated from the Central Brazil, incubated at 32 ± 0.5°C or 27 ± 1°C. In addition, the virulence was assessed for each strain of R. microplus engorged females. IP119, Metarhizium anisopliae s.s., was selected among the other isolates for virulence tests against R. microplus submitted with stressful temperature condition (32 ± 0.5°C) or low relative humidity (RH = 75%) to present considerable heat tolerance and virulence in the screening tests. Tests with conidia formulated in oil-water emulsion (liquid vaseline 5%), carboxymethylcellulose gel or emulgel 5% were conducted to evaluate the conidial thermotolerance when exposed to 45°C for 4 hours or 6 hours, and to test the efficacy of formulated to control engorged female R. microplus the stressful conditions of temperature (32°C) and low humidity (RH = 75%). It was concluded that a constant temperature of 32°C or exposure for a few hours at 45°C is stressful condition for the isolates. The conidia IP119 when bathed in liquid vaseline jelly were more tolerant of exposure to 45°C for 4 hours. IP119 was shown to be virulent for engorged female R. microplus, being the oil-water emulsion 5% of the most effective formulation.Item Bacillus thuringiensis para controle de Musca domestica: seleção de estirpes e avaliação do perfil de hemócitos em larvas(Universidade Federal de Goiás, 2017-06-28) Stein, Ana Elisabeth Korte; Pontes , Rose Gomes Monnerat Solon de; http://lattes.cnpq.br/9239372895737198; Fernandes, Éverton Kort Kamp; http://lattes.cnpq.br/2135541732341157; Fernandes, Éverton Kort Kamp; http://lattes.cnpq.br/2135541732341157; Monteiro, Caio Marcio de Oliveira; Angelo, Isabele da CostaMusca domestica is considered one of the most harmful pests to the egg industry due to its high reproductive capacity and great potential of acting as a mechanical vector of many pathogenicagens to the egg-laying hens. Currently, the society is interested in alternative methods for pest control, once the constant and indiscriminate use of chemical products has caused intoxication to humans and domestic animals. Also, the irregular use of chemicals has selected resistant fly populations, accumulated chemical residues in the environment and food, and eliminated insects important to the natural control of flies. In order to develop alternative methods to control M. domestica, this study investigated Bacillus thuringiensis strains able to act as promising biological control agents. Twenty two strains of B. thuringiensis were tested in a screening assay for selecting the most virulent ones; one third of them were virulent to M. domestica larva, and two of them were selected for the next tests. Bioassays were conducted with the strains S601 and S599 to determine the larval treatment efficiency; they showed efficiency to control larva of M. domestica in all concentrations tested. The current study also characterized the hemocytes from hemolymph of larva of M. domestica by their identification and quantification; also, we evaluated the effect of the strain S599 of B. thuringiensis infection to the total hemocytes counts, and number and size of hemocyte types, using optical microscopy. Collections were made at the times of 12, 24, 48 and 72 hrs. Five hemocyte types were found: prohemocytes, plasmatocytes, granulocytes, oenocytoids and spherulocytes, in which plasmatocytes were found in superior number. Prohemocytes were the smallest cells observed, and oenocytoids were the biggest ones. The treatment of M. domestica larva with B. thuringiensis did not change morpholigical characteristics of hemocytes, as well as it did not modify the counts of hemocyte types. The total number of hemocytes in the hemolymph, however, increased significantly 48 hours after the larval treatment. B. thuringiensis strains selected in the current study are promising agents for biological control of M. domestica.