Mestrado em Biologia da Relação Parasito-Hospedeiro (IPTSP)

URI Permanente para esta coleçãohttp://200.137.215.59/tede/handle/tde/214

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Isolamento, caracterização biológica e estabilidade de bacteriófagos líticos e sua compatibilidade com bactérias ácido-láticas (BALs) visando o biocontrole de Salmonella Enteritidis
(Universidade Federal de Goiás, 2025-08-25) Freitas, Daniela Borges; Vieira, José Daniel Gonçalves; http://lattes.cnpq.br/1742731776579730; Vieira, José Daniel Gonçalves; http://lattes.cnpq.br/1742731776579730; Silva, Carla Afonso da; http://lattes.cnpq.br/7673897995590123; Vasconcelos, Lara Stefânia Netto de Oliveira Leão; http://lattes.cnpq.br/5423949965995995
Embargada.
Avaliação histopatológica da glândula digestiva do caramujo Biomphalaria glabrata (Say, 1818) após exposição a nanopartículas verdes
(Universidade Federal de Goiás, 2025-08-28) Ferreira, Luiz Felipe da Silva; Rocha, Thiago Lopes; http://lattes.cnpq.br/6325937100056775; Rocha, Thiago Lopes; http://lattes.cnpq.br/6325937100056775; Rodrigues Filho, Juscelino; http://lattes.cnpq.br/7721100359125364; Tallarico, Lenita de Freitas; http://lattes.cnpq.br/7300603266478611
Embargado
Adenovírus humano em diferentes espécimes clínicos de crianças internadas em unidade de terapia intensiva em Goiânia-Go
(Universidade Federal de Goiás, 2025-08-29) Sousa, José Arthur Silva e; Souza, Menira Borges de Lima Dias e; http://lattes.cnpq.br/0054562567103606; Souza, Menira Borges de Lima Dias e; Matos, Marcia Alves Dias de; Freitas, Erika Regina Leal de
Resumo
Implicações da adesina iceA de Helicobacter pylori para os desfechos clínicos de pacientes dispépticos
(Universidade Federal de Goiás, 2025-06-05) Silva, Mariana Oliveira; Rocha, Viviane Lopes; http://lattes.cnpq.br/7165684017307847; Barbosa, Mônica Santiago; http://lattes.cnpq.br/2417424087790287; Barbosa, Mônica Santiago; http://lattes.cnpq.br/2417424087790287; Santos, Rodrigo da silva; http://lattes.cnpq.br/4806187026900959; Rasmussen, Lucas Trevizani; http://lattes.cnpq.br/9152072043915486
Embargada
Bioprospecção de bactérias de ambientes cavernícola com potencial antimicrobiano
(Universidade Federal de Goiás, 2025-06-26) Lima, Michel Gentile; Vieira, José Daniel Gonçalves; http://lattes.cnpq.br/1742731776579730; Vieira, José Daniel Gonçalves; http://lattes.cnpq.br/1742731776579730; Amaral, André Corrêa; http://lattes.cnpq.br/8801299423520104; Anunciação, Carlos Eduardo; http://lattes.cnpq.br/4354412874919580
Embargado
Toxicidade de nanopartículas de prata funcionalizadas com ácido mercaptosuccínico e químicos ambientais no caramujo Biomphalaria glabrata (Say, 1818)
(Universidade Federal de Goiás, 2024-12-13) Leão, Gabrielly Rodrigues; Silva, Luciana Damacena; http://lattes.cnpq.br/3800305083164308; Rocha, Thiago Lopes; http://lattes.cnpq.br/6325937100056775; Rocha, Thiago Lopes; http://lattes.cnpq.br/6325937100056775; Tallarico, Lenita de Freitas; http://lattes.cnpq.br/7300603266478611; Rodrigues Filho, Juscelino; http://lattes.cnpq.br/7721100359125364
Embargado
Cultivo submerso de Metarhizium anisopliae (Metsch.) Sorokin: Estratégias para a otimização da produtividade e secagem de blastosporos
(Universidade Federal de Goiás, 2025-03-12) Peixoto, Marciel José; Mascarin, Gabriel Moura; http://lattes.cnpq.br/3162686608683670; Fernandes, Éverton Kort Kamp; http://lattes.cnpq.br/2135541732341157; Fernandes, Éverton Kort Kamp; http://lattes.cnpq.br/2135541732341157; Faria, Marcos Rodrigues de; http://lattes.cnpq.br/3612465581571231; Lima, Valesca Henrique; http://lattes.cnpq.br/4870259780815703
Embargado.
Análises do papel do TLR7 em infecções por Leishmania (Viannia) guyanensis ou Leishmania (Viannia) braziliensis
(Universidade Federal de Goiás, 2025-03-07) Torres, Ana Luisa Macedo das Mercez; Dias, Fátima Ribeiro; http://lattes.cnpq.br/5741031258926403; Dias, Fátima Ribeiro; Carmo Neto, José Rodrigues do; Fonseca, Simone Gonçalves da
Embargado.
Galleria mellonella como modelo experimental para avaliar os efeitos nocivos de cocaína: resposta imune e comportamental
(Universidade Federal de Goiás, 2025-02-07) Noleto, Ludmylla Prateado de Assis Costa; Amaral, André Corrêa; http://lattes.cnpq.br/8801299423520104; Amaral, André Corrêa; Garcia, Luane Ferreira; Fernandes, Éverton Kort Kamp
Galleria mellonella (“wax moth”) has been used to study the pathogenesis of microorganisms, the toxicity of antimicrobials and physical and chemical agents. The use of this insect as a model system in animal research is possible because the immune system of G. mellonella shares functional and structural homology with the innate immune system of vertebrates. The present study evaluated the use of G. mellonella larvae as a model organism to investigate the effects and toxicity of cocaine using negative control groups (PBS), positive control groups (DMSO) and test groups with different concentrations of cocaine. A pilot test was carried out with concentrations of 5 mg/mL, 7.5 mg/mL, 10 mg/mL and 20 mg/mL of cocaine and behavioral changes were observed for 120 min. Based on the survival data, concentrations of 10 mg/mL, 20 mg/mL, 40 mg/mL, 80 mg/mL and 200 mg/mL of cocaine were standardized to assess the health of the larvae, the percentage of survival and death and the average lethal concentration (LC50), which were analyzed over 120 h. To assess the immune response and tissue damage, concentrations of 10 mg/mL, 20 mg/mL, 40 mg/mL and 80 mg/mL were used 24 h after drug administration. The behavioral changes observed were: immediate and intermittent hyperexcitation (rhythmic tremors, sweating and regurgitation), followed by paralysis with sporadic spasms and, finally, complete paralysis, not necessarily followed by death. Cocaine did not inhibit silk production and cocoon formation (except at a concentration of 200 mg/mL, where all the individuals died less than 24 hours after administration of the drug). The death of at least one individual was observed in the test groups during the 120 hours of the experiments. The administration of different concentrations of cocaine produced immune responses in G. mellonella larvae: with regard to cellular immunity, activation of granulocytes, spherulocytes and oenocytoids was observed; and with regard to humoral immunity, activation of the pro-phenoloxidase cascade was observed, with subsequent melanization at the highest concentrations of the drug (80 mg/mL and 200 mg/mL) in nearly half of the body surface of the lower region (caudal). There was tissue damage in the digestive system of G. mellonella larvae characterized by a reduction in the haemocyte layer and rupture of part of the intestine (middle or final) which may be related to the administration of different concentrations of cocaine. The LC50 was 73.53 mg/mL. Cocaine had an immunosuppressive and immunotoxic effect on G. mellonella larvae. In conclusion, G. mellonella larvae can be used as a model system for initial studies of the acute effects of cocaine, with the potential for studies of chronic abuse (addiction).
Avaliação do papel da opsonização na suscetibilidade de macrófagos murinos à infecção pelas diferentes espécies de Leishmania
(Universidade Federal de Goiás, 2023-03-31) Polonski, Daniella Cristina Silva; Oliveira, Milton Adriano Pelli de; http://lattes.cnpq.br/2152513705182408; Oliveira, Milton Adriano Pelli de; Gomes, Rodrigo Saar; Borges, Arissa Felipe
Leishmaniasis are a set of parasitic diseases caused by a protozoa of the Leishmania genus. Clinical manifestations vary according to the Leishmania species and host immune response. Among of the possible clinical manifestation of leishmaniasis, according to the symptomatology, three stand out: visceral, cutaneous and mucosal leishmaniasis. Macrophages are key cells in the prognosis of Leishmania infection. M1 macrophages stimulate the Th1 response are associated with leishmanicidal activity; the M2 macrophages are activated mainly by Th2 lymphocytes are associated with the growth and survival of the parasites. The M2b subtype regulates the immune response and the inflammatory reaction against intracellular parasites and is related to the humoral response. The present work evaluated the susceptibility of different species of Leishmania: Leishmania (V.) braziliensis, Leishmania (L.) major, Leishmania (L.) infantum and Leishmania (L.) amazonensis to in vitro infections, using macrophages from BALB/c and C57BL/6. Murine macrophages were cultured and infected with promastigotes from different Leishmania species, opsonized or not with anti-leishmania antibody. The infection was evaluated by the promastigotes retrieval method at the points: 3 h, 24 h, 72h and 120h. We found that C57BL/6 macrophages are resistant to infection by L. (V.) braziliensis and L. (L) amazonensis. BALB/c mice show susceptibility to L. (L) infantum. Both C57BL/6 and BALB/c infected with L. (L.) major parasites show susceptibility to the parasite. Both in infections with L. major and L. infatum, it was observed that the association with antibodies is more related to the reproduction of the parasites and survival inside the macrophages than to the killing of parasites We therefore conclude that opsonization with anti-leishmania can modulate the macrophage to a characteristic M2b profile, favoring infection.
Associação entre polimorfismos genéticos do hospedeiro e suscetibilidade à infecção por Helicobacter pylori: uma revisão sistemática e meta-análise
(Universidade Federal de Goiás, 2024-08-08) Santos, Hellen Christina de Oliveira; Santos, Rodrigo da Silva; http://lattes.cnpq.br/4806187026900959; Barbosa, Mônica Santiago; http://lattes.cnpq.br/2417424087790287; Barbosa, Mônica Santiago; Gomes, Rodrigo Saar; Rocha, Viviane Lopes
Conteúdo embargado
Papel da sinalização purinérgica na infecção por Leishmania braziliensis
(Universidade Federal de Goiás, 2025-02-21) Paula, Wesley Lima de; Gomes, Rodrigo Saar; http://lattes.cnpq.br/8840051460928720; Gomes, Rodrigo Saar; Dias, Fátima Ribeiro; Borges, Pauline Martins Leite
Leishmaniasis are infectious-parasitic diseases caused by protozoa of the genus Leishmania. The infection process causes the release of ATP by cells and tissues. Extracellular ATP can be converted into adenosine by the enzymes CD39 and CD73. Adenosine has anti-inflammatory effects by binding to P1 receptors, especially A2A and A2B receptors. This work evaluated the role of purinergic signaling in the human immune response to Leishmania braziliensis. Data obtained from transcriptome analyzes (GEO GSE55664) show that patients with localized cutaneous leishmaniasis (LCL; n = 21), caused by L. braziliensis, showed higher expression of CD39, CD73 and A2A receptor than healthy controls (n = 7). On the other hand, A2BR expression was lower in patients with LCL. In patients, we found positive correlations between the expression of A2AR and IL1B, IL6 and IL32, while the expression of A2BR is negatively correlated with the expression of the genes of these cytokines. Parasitism rates were evaluated in human THP-1 macrophages infected by Leishmania braziliensis-GFP, which also expressed higher levels of A2AR, in the presence and absence of selective A2AR antagonists, at 24 and 48 hours. There was no change in parasitism rates in macrophages and in the production of cytokines and ROS. However, when the enzyme adenosine deaminase (ADA) is inhibited, the A2AR antagonist significantly reduces infection rates. These data suggest that L. braziliensis infection alters the expression of enzymes and receptors involved in purinergic signaling, and these changes are important in modulating the immune response during LCL.
Análise funcional da 7-desidrocolesterol redutase na resposta imune durante a infecção por Leishmania spp.
(Universidade Federal de Goiás, 2024-02-24) Mota, Carlos Henrique da Silva; Gomes, Rodrigo Saar; http://lattes.cnpq.br/8840051460928720; Rodrigo Saar; Gomes, Rodrigo Saar; Fonseca, Simone Gonçalves da; Silveira, Murilo Barros
Leishmaniasis is caused by protozoa of the genus Leishmania spp. and is a neglected parasitic disease with a broad clinical spectrum, whose treatments are toxic and present failures and resistance. Leishmania braziliensis (Lb) and Leishmania amazonensis (La) are highly prevalent species in Brazil. Cholesterol metabolism affects immune responses. 7-dehydrocholesterol reductase (DHCR7) converts 7-dehydrocholesterol (7-DHC) into cholesterol and this enzyme regulates the polarization of macrophages towards the M2 profile. The main objective is to evaluate the role of DHCR7 in the immune response and in the control of Leishmania spp. infections. Human macrophages derived from THP-1 cells were infected with L. amazonensis or L. braziliensis expressing green fluorescent protein (GFP) for 24 h and treated with pharmacological inhibitors of DHCR7 (tamoxifen and AY9944) for an additional 24 h for flow cytometric analysis. Public transcriptome analysis demonstrated that DHCR7 is less expressed in lesions from patients with cutaneous leishmaniasis compared with skin from healthy controls. DHCR7 is negatively correlated with the expression of pro-inflammatory molecules in lesions from patients. Inhibition of DHCR7 reduced infection by both Leishmania species in human macrophages. Furthermore, DHCR7 inhibitors promoted an increase in the production of reactive oxygen intermediates (ROS) and in the production of TNF and IL-1β by human macrophages infected with Leishmania spp, assessed by CBA in the supernatant. These data suggest that DHCR7 inhibitors control Leishmania spp. infection and pave the way for new therapeutic approaches for this neglected disease.
Atividade biológica de óleos essenciais em leveduras do complexo Cryptococcus neoformans
(Universidade Federal de Goiás, 2013-06-24) Abrão, Fernando Yano; Souza, Lúcia Kioko Hasimoto e; Oliveira, Milton Adriano Pelli de; Paula, José Realino de
Species of the complex Cryptococcus neoformans can cause infection in both healthy individuals and immunocompromissed patients, especially in AIDS patients, in which meningoencephalitis is the main clinical manifestation. There are few available antifungals for cryptococcosis treatment and all of them present high toxicity, besides the reports of resistance. In this context, the natural products are important source in the search for new antifungal compounds. In this study, it was evaluated the biological activity of the essential oils (EO) of Pelargonium graveolensis, Syzygium aromaticum and Cymbopogon flexuosus. The antifungal activity against Cryptococcus isolates was determined by minimum inhibitory concentration (MIC) by microdilution broth. Once the antifungal activity was measured, its cytotoxic activity were evaluated in L929 fibroblastic cell line in an in vitro assay. The mechanism of action of the EOs was analyzed by flow cytometry, using propidium iodide as fluorescent marker for lesions in the cell membrane, and FUN-1 ([2-chloro-4-(2,3-dihydro-3-methyl- (benzo-1,3-thiazol-2-yl)-methylidene)-1-phenylquinolinium iodide]), to analyze alterations of the cellular metabolism. Furthermore, the amount of ergosterol in the fungal membrane was evaluated to ascertain if there were modifications on its synthesis due to the exposition to the EOs. All the EOs presented antifungal activity, P. graveolensis and S. aromaticum with MICs between 128 and 256 µg/mL, while C. flexuosus obtained MICs from 8 to 32 µg/mL, whereas for these values, none EO has shown cytotoxicity in L929 fibroblastic cell line. The metabolic inhibition of fungal cells and membrane lesion were observed as the mechanism of action of all the evaluated EOs, however, only the EO of S. aromaticum was able to decrease the ergosterol amount in the fungal membrane. This study reveals the antifungal potential of the EOs of P. graveolens, S. aromaticum e C. flexuosus, due to their activity in isolated samples of Cryptococcus and relative toxicological safety.
Análise in silico do papel do receptor imune TREM-1 na infecção pelos Norovírus murino e humano
(Universidade Federal de Goiás, 2024-01-22) Colmenares, Mike Telemaco Contreras; Sales, Marcelle Figueira Marques da Silva; http://lattes.cnpq.br/0477630359032513; Campos, Helioswilton Sales de; http://lattes.cnpq.br/1386621024118393; Campos, Helioswilton Sales de; Dias, Fátima de Rivero; Silva, Marcos Vinicius da
In humans, Norovirus (NoV) is one of the main causes of acute diarrheal disease (ADD). Due to the limitations of NoV cultivation, some molecular aspects of its interaction with the host's immune system remain unknown. The triggering receptor expressed on myeloid cells 1 (TREM-1) is associated with the amplification of inflammatory responses and the progression of infections, including viral infections. Thus, we believe that TREM-1 may be involved in the NoV infection. Initially, we investigated the expression of Trem1 and the genes involved in its pathway, in transcriptomic data bank of public domain. In experimental infection with murine Norovirus (MNoV), the expression of Trem1 was increased. We also observed that there is a co-expression of Trem1 and genes involved in the pyroptosis pathway, when compared to those in the apoptosis pathway. The in silico protein-protein interactions were assessed by molecular docking simulations between the Ig-like domain of murine TREM-1 and the P domain of the MNoV VP1 protein. The murine TREM-1 recognized the conserved C´-D´ antigen that is present in the murine VP1. In this regard, and based on phylogenetic criteria, different structures of the VP1 protein of NoV GII.4 strains from different years (1987, 2010, 2012, 2014, 2016 and 2019) were modeled. We performed docking and molecular dynamics (MD) simulations to determine the in silico interaction between the VP1 protein of NoV GII.4 and the Iglike domain of human TREM-1. The DM simulations suggest that there is a basic interaction between human TREM-1 and the NoV VP1 protein, regardless of the year of isolation. Interestingly, we observed changes in the participation of the different complementarity determining regions (CDRs) of TREM-1 when interacting with the domains of the VP1 protein, highlighting the participation of CDR3. Our data strongly suggests the involvement of TREM-1 in the recognition of NoV and its participation in the physiopathology of ADD caused by NoV.
Avaliação da resposta imune após vacinação para covid-19
(Universidade Federal de Goiás, 2023-08-28) Masson, Letícia Carrijo; Fonseca, Simone Gonçalves da; http://lattes.cnpq.br/3823367099967701; http://lattes.cnpq.br/3823367099967701; Fonseca, Simone Gonçalves da; Kipnis, Ana Paula Junqueira; Oliveira, Milton Adriano Pelli de
Immune responses after COVID-19 vaccination should be evaluated in different populations around the world. This study compared antibody responses induced by ChAdOx1 nCoV-19, CoronaVac, and BNT162b2 vaccines. Blood samples from vaccinees were collected pre- and post-vaccinations with the second and third doses. The study enrolled 78 vaccinees, of whom 62.8% were women, with the following median ages: 26 years—ChAdOx1 nCoV-19; 40 years—CoronaVac; 30 years—BNT162b2. Serum samples were quantified for anti-Receptor Binding Domain (RBD) IgG and anti-RBD IgA and anti-spike IgG by ELISA. After two vaccine doses, BNT162b2 vaccinees produced higher levels of anti-RBD IgA and IgG, and anti-spike IgG compared to ChAdOx1 nCoV-19 and CoronaVac vaccinees. The third dose booster with BNT162b2 induced higher levels of anti-RBD IgA and IgG, and anti-spike IgG in CoronaVac vaccinees. Individuals who reported a SARS-CoV-2 infection before or during the study had higher anti-RBD IgA and IgG production. In conclusion, two doses of the studied vaccines induced detectable levels of anti-RBD IgA and IgG and anti-spike IgG in vaccinees. The heterologous booster with BNT162b2 increased anti-RBD IgA and IgG and anti-spike IgG levels in CoronaVac vaccinees and anti-RBD IgA levels in ChAdOx1 nCoV-19 vaccinees. Furthermore, SARS-CoV-2 infection induced higher anti-RBD IgA and IgG levels in CoronaVac vaccinees. Novembro
Quitinases humanas e infecções fúngicas
(Universidade Federal de Goiás, 2023-02-27) Farias, Adriane Nunes; Amaral, André Corrêa; http://lattes.cnpq.br/8801299423520104; Amaral, André Corrêa; Silva, Daniela de Melo e; Soares, Renata de Bastos Ascenço
This work deals with a systematic review of the intrinsic mechanisms used by humans to fight fungal infections, focusing on chitinases, discussing the main enzymes and gene polymorphism with them involved. Fungal infections are increasingly common in the world, being considered one of the main causes of death in immunocompromised and immunosuppressive patients. Diagnoses and treatments for these infections are still limited and prophylaxis through vaccines is still far from being achieved. Fungi, in general, have chitin in their cell wall, the second most abundant polymer in nature, not found in humans. However, enzymes capable of degrading it, called chitinases, are synthesized by them. Human chitinases are found in the family 18 of glycosyl hydrolases, of which only chitotriosidase (QT), mammalian acid chitinase (AMCase) and di-N-acetyl-chitobiase have chitinolytic activity. QT and AMCase are the most reported chitinases in humans and differ mainly in terms of the chitin cleavage region and the immune response that leads to their expression. The enzymatic activity of chitinases has been observed in several diseases, including fungal infections such as candidiasis, mycetoma and aspergillosis. It is believed that these enzymes act on the innate immune system against pathogenic fungi and their absence or inactivity leads to susceptibility to these organisms. Polymorphisms associated with genes encoding chitinases, especially QT, have been recently described. The duplication of 24 bp in exon 10 of the gene that expresses QT results in a protein with enzyme deficiency. Therefore, this review seeks to gather existing data on fungal infections, their relationship with chitinases, discussing the influence of gene polymorphisms on the chitinolytic activity of these enzymes against pathogenic fungi.
Avaliação do processo cicatricial utilizando biomembrana de látex de hancornia speciosa e regederm® em feridas de espessura total induzidas experimentalmente em ratos
(Universidade Federal de Goiás, 2016-09-02) Martins, Kassya Lopes Epaminondas; Reis, Paulo Roberto de Melo; http://lattes.cnpq.br/7729613632937834; Lino Junior, Ruy de Souza; http://lattes.cnpq.br/0372118837748010; Lino Junior, Ruy de Souza; Mrué, Fátima; Galdino Júnior, Helio
The use of Hevea brasiliensis latex in the wound treatment have been widely studied as a biomembrane bandage. Nowadays it is comercially available as Regederm®, a gel-ointment indicated in the use of cutaneous wounds treatment. Also the Hevea brasiliensis látex has been used in the wound healing because of its angiogenic and anti-inflammatory ativities. The presente study aimed the comparison of the healing effects of H. speciosa biomembrane and Regederm®. 70 Wistar rats divided in G1 – control group, treated with physiologic solution, G2 – treated with Regederm®, G3 – treated with H. speciosa biomembrane, G4 – mixed treatment with Regederm® and physiologic solution. The euthanasia occurred at 3, 7, 14 and 21 days after the induction (DAI) of the incisional injury. Morphometric, microscopic and macroscopic analysis were performed evaluating the general pathologic processes. It was possible to observe that the H. speciosa biomembrane decreased necrosis and inflammation during the inflammatory and proliferative phases of the healing process. The angiogenesis observed was similar to the one found in the Regederm® treated group. During the maturation phase of the healing process the Regederm® continued to stimulate the angiogenesis which was not observed in the H. speciosa biomembrane treated group. There was an intense stimulus of fibroblastos with the Regederm® treated group and the mixed treatment group in the proliferative and maturation phases of the healing process while in the H. speciosa biomembrane treated group there was an inhibition of these cells. It was possible to observe that in the Regederm® treated group there was a continuous inflammation of the tissue during the maturation phase of the healing process. Therefore it is possible to conclude that Regederm® contributed to the healing process in its inflammatory and proliferative phases but tended to perpetuate the inflammation process. While the H. speciosa biomembrane decreased necrosis and inflammation during the initial phases of the healing process, stimulated angiogenesis and interrupted these stimuly during the maturation phase of the healing process. The mixed treatment group decreased the delayed angiogenic effects of Regederm® however the inflammation was sustained leading to a delay in the healing process with less wound contraction.
Indicadores de contaminação viral em amostras de água consumida em regiões rurais de Goiás
(Universidade Federal de Goiás, 2022-11-28) Bordoni, Graziela Picciola; Carneiro, Lilian Carla; http://lattes.cnpq.br/6506744224041777; Carneiro, Lilian Carla; Albuquerque, Antônio João Carvalho de; Oliveira, Bruno Francesco Rodrigues de
Rural communities are located far from urban centers, where the adequate supply of potable water is hampered. In the lack of a public distribution network, water is obtained from surface and groundwater sources, where the microbial quality is often unknown. Goiás has 9.7% of the population in rural areas and previous studies carried out in some of these rural and traditional communities have already demonstrated the vulnerability of water for the consumption of these residents by waterborne pathogens, such as enteric viruses. Therefore, the aim of the work was to continue the evaluation of fecal contamination of water samples from individual and collective sources, from 24 communities in rural areas of the State of Goiás. 160 samples were collected, whose water sources were shallow tubular well, deep tubular well, dug well and spring, surface water, rainwater stored in cisterns and water truck. The enteric viruses Human Adenovirus (HAdV), Rotavirus (RV) and Enterovirus (EV) were used as indicators of fecal contamination, analyzed by qPCR. In all, 30% of the samples showed signs of fecal contamination. Detection rates were 20% for RV, 9.4% for HAdV and 4.4% for EV. The geometric means of concentration of these indicators were 1.5x106 GC/L, 1.9x106 GC/L and 7.1x105 GC/L, respectively. RV was the most prevalent viral indicator in underground sources, being statistically associated with shallow tubular wells. In spring samples, HAdV stands out with 11.8%. In the cistern samples, both HAdV and RV were found in the same prevalence. These results reveals that the analyzed population is vulnerable to waterborne diseases caused by enteric pathogens, serving to guide future decisions and improve daily water treatment practices in these communities.
Toxicidade reprodutiva e resposta de múltiplos biomarcadores no caramujo Biomphalaria glabrata (Say 1818) após exposição crônica às nanopartículas de óxido de ferro (γ-Fe2O3) funcionalizadas com ácido glucônico
(Universidade Federal de Goiás, 2020-03-17) Caixeta, Maxwell Batista; Silva, Luciana Damacena; http://lattes.cnpq.br/3800305083164308; Rocha, Thiago Lopes; http://lattes.cnpq.br/6325937100056775; Rocha, Thiago Lopes; Monteiro, Caio Márcio de Oliveira; Fernandes, Éverton Kort Kamp
Nanotechnology has been applied to control parasites and intermediate hosts of etiologic agents of neglected global and tropical diseases, such as schistosomiasis. The parasite Schistosoma mansoni is the etiological agent of this disease in Brazil, and snails Biomphalaria spp. act as intermediate host. The use of nanomaterials (NMs) as molluscicidal agents is promising due to their specific properties that allow internalization, greater reactivity and specificity to the snail, in addition to the ease of production, and the possibility of removal from the environment. In this sense, the aim of this work was to evaluate the potential molluscicidal activity of gluconic acid - functionalized iron oxide nanoparticles (IONPs) in the snail Biomphalaria glabrata. Initially, a bibliometric analysis associated with a systematic review of the literature identified that different NMs were able to induce oxidative stress, genotoxicity, mutagenicity, embryotoxicity, reproductive and transgenerational toxicity, immunotoxicity, mortality and behavioural changes in different species of gastropods. Regarding bioassays, after chronic exposure (28 days) of B. glabrata to different concentrations of IONPs and FeCl3 (1.0; 2.5; 6.2 and 15.6 mg L-1), a high bioaccumulation of iron by IONPs in visceral mass of the snail compared to the iron ions and the control group was observed. Likewise, there was a high frequency of behavioural changes in snails exposed to IONPs when compared to their ionic counterpart and to the control group. Both forms of Fe reduced fertility, while mortality and reduced snail’s fertility were observed only after exposure to IONPs at 15.6 mg L-1. The general results indicated behavioural impairments and reproductive toxicity, associated with the bioaccumulation of IONPs in B. glabrata. This study emphasizes that metal-based nanoparticles are potential molluscicidal agents.

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