Mestrado em Biologia da Relação Parasito-Hospedeiro (IPTSP)

URI Permanente para esta coleçãohttp://200.137.215.59/tede/handle/tde/214

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Desenvolvimento de formulação seca à base de Metarhizium humberi com terra diatomácea para controle de Aedes aegypti
(Universidade Federal de Goiás, 2026-02-26) Oliveira Júnior, Wellington Pereira de; Luz, Wolf Christian; http://lattes.cnpq.br/1104009511235835; Luz, Wolf Christian; Bezerra, Jadson Luiz; Santos , Solange Xavier dos
Entomopathogenic fungi are promising biological agents for controlling populations of Aedes aegypti, the main vector of medically important arboviruses in tropical and subtropical regions. Prolonged periods of low humidity, common during the dry season in regions where this mosquito occurs, impair the development of the extracuticular stages. After adhering to the cuticle, conidia require high humidity to germinate and infect the host. Conidia formulations with specific additives aim to increase the insecticidal effect, in addition to improving the conditions for propagule development. The definition of additives is crucial for the development and success of a formulation. Diatomaceous earth (DE) is an inert powder obtained from microalgae fossils, which, when in contact with the insect's cuticle, causes abrasive damage and adsorbs lipids, leading to the evaporation of liquids from the insect and, consequently, death by dehydration. Because of this, it is possible that the damage caused to the cuticle, together with the extravasated moisture, favors the penetration and germination of conidia, even in low humidity environmental conditions. The present study sought to define an appropriate concentration of TD as an additive in a formulation of Metarhizium humberi conidia for the control of A. aegypti adults in low humidity conditions. Under laboratory conditions of 35 ± 2 or > 98% RH (positive control), TD was tested at six concentrations ranging from 0.05 to 1 mg/cm2 , and based on the results found, a sublethal operational concentration was determined for testing with conidia. TD had a high adulticide effect at low humidity. The LC30 of TD at 3 and 5 days were 0.04 (0.006‒0.08) mg/cm2 and 0.03 (0.007‒0.06) mg/cm2 , respectively, and the TL50 ranged from 1.5 (0.9‒2) days for 1 mg/cm2 to 6.5 (4.1‒9) days for 0.05 mg/cm2 . At high humidity, the LC30 at 10 days was 0.46 (0.13‒4.30) mg/cm2 , and the TL50 was 11 (6.4‒24.5) days for 1 mg/cm2 . Based on applicability in a powder formulation, 0.02 mg TD/cm2 was defined as the sublethal concentration of the additive. Under the same conditions, conidia were tested at three concentrations ranging from 3.3 x 105 to 3.3 x 107 conidia/cm2 , formulated or not with 0.02 mg TD/cm2. At low humidity, the additive enhanced the adulticide effect of conidia. The average mortality caused only by conidia in 3 and 5 days was 0% and 0% for 3.3 x 105 conidia/cm2, 0% and 30 ± 34.6% for 3.3 x 106 conidia/cm2, and 25 ± 17.3% and 90 ± 11.5% for 3.3 x 107 conidia/cm2. For conidia + TD, the average mortality rates in the same period were 12.5 ± 9.5% and 35 ± 17.3% for 3.3 x 105 conidia/cm2 , 0% and 50 ± 20% for 3.3 x 106 conidia/cm2 , and 20 ± 8.1% and 77.5 ± 17% for 3.3 x 107 conidia/cm2 . At high humidity, conidia without TD showed high mortality rates, and the addition of TD also increased theadulticide effect. The mortality rates for conidia alone at 3 and 5 days were 0% and 10 ± 11.5% for 3.3 x 105 conidia/cm2 , 0% and 55 ± 28.8% for 3.3 x 106 conidia/cm2 , and 65 ± 28.8% and 90 ± 11.5% for 3.3 x 107 conidia/cm2 . For conidia + TD, the mean mortality rates in the same period were 0% and 22.5 ± 12.5% for 3.3 x 105 conidia/cm2 , 0% and 67.5 ± 27.5% for 3.3 x 106 conidia/cm2 , and 67.5 ± 22.1% and 100% for 3.3 x 107 conidia/cm2 . Based on biorationality, mortality averages, LC, and LC, the most appropriate conidia concentration + 0.02 mg TD/cm2 for the formulation was 1.5 x 106 conidia/cm2 . This formulation is effective in both low and high humidity conditions in laboratory conditions, and in future studies, it will be tested in semi-field and field conditions.
Desenvolvimento de uma ferramenta de identificação e estimativa de contagem de moscas-brancas no feijoeiro
(Universidade Federal de Goiás, 2026-02-26) Gil, Henric Pietro Vicente; Hirose, Edson; http://lattes.cnpq.br/7892692886810778; Pinheiro, Patrícia Valle; http://lattes.cnpq.br/1705855482738430; Pinheiro, Patrícia Valle; Lobo Junior, Murillo; Christian, Arthur
Based on the principles of One Health, food security requires sustainable practices that reduce pesticide use without compromising production. The common bean (Phaseolus vulgaris), a widely consumed food especially in Latin America and African countries, is affected by viral diseases transmitted by the whitefly Bemisia tabaci (Hemiptera: Aleyrodidae), such as the bean golden mosaic virus, which can cause severe losses. The control of these diseases depends on vector management, which requires more efficient and lower-cost monitoring methods. In this context, this study aimed to develop a computer vision tool capable of assisting in the process of counting whitefly nymphs on common bean leaves, to facilitate monitoring and integrated vector management actions. The methodology involved the development of a mobile application integrated with the YOLO11 computer vision model, trained on an image dataset composed of 1,352 photographs of nymphs on soybean and bean leaves, randomly divided in an 8:1:1 ratio into training, testing, and validation sets. The model was then used to perform detections on bean leaves. The results were organized into three detection scenarios: (i) mixed resolution with macro images, (ii) low resolution with macro images, and (iii) mixed resolution without macro use. For each scenario, considering a confidence threshold of 0.5, the model achieved the following Precision, Recall, and mAP50 values on the validation set: (0.7735, 0.7215, 0.82071), (0.87024, 0.7014, 0.7554), and (0.83371,0.7393, 0.82189), respectively. Based on the results obtained, it can be concluded that the proposed tool presents satisfactory performance for the detection and counting of whitefly nymphs on bean leaves. However, its performance is influenced by image quality, achieving better results with higher resolution images captured in macro mode. Nevertheless, the tool shows potential as an auxiliary instrument for population monitoring of the vector and for supporting further research in the management and control of this insect.
Avaliação da circulação de vírus respiratórios no município de Goiânia-GO, no período 2022-2023
(Universidade Federal de Goiás, 2026-02-27) Sousa, Aline Maria Luz de; Fiaccadori , Fabíola Souza; http://lattes.cnpq.br/0993842409303174; Fiaccadori , Fabíola Souza; Carneiro, Megmar Aparecida dos Santos; Souza, Menira Borges de Lima Dias e
Embargo
Investigação molecular dos vírus dengue e zika em amostras de soro e líquor de pacientes com suspeita de distúrbio neurológico atendidos em hospital de referência em Goiânia
(Universidade Federal de Goiás, 2025-12-19) Freitas, Thalita de Paula Medeiros Mota; Fiaccadori, Fabíola Souza; http://lattes.cnpq.br/0993842409303174; Souza , Menira Borges de Lima Dias de; http://lattes.cnpq.br/0054562567103606; Souza, Menira Borges de Lima Dias e; Sales, Marcelle Figueira Marques da Silva; Siqueira, Cláudio Morais
Embargado
Cenário clínico e laboratorial de toxoplasmose congênita detectado pelo Teste do pezinho em recém-nascidos encaminhados a um centro de referência em Goiânia, Goiás
(Universidade Federal de Goiás, 2025-08-28) Mateus, Fernando Oliveira; Castro, Ana Maria de; http://lattes.cnpq.br/9232309971000621; Vinaud, Marina Clare; http://lattes.cnpq.br/1921551651088660; Vinaud, Marina Clare; http://lattes.cnpq.br/1921551651088660; Storchilo, Heloisa Ribeiro; http://lattes.cnpq.br/3543813852521316; Rezende, Hanstter Hallison Alves; http://lattes.cnpq.br/4982752673858886
Resumo
Infecção pelo virus da hepatite C em indivíduos privados de liberdade no Estado de Goiás: prevalência e fatores associados
(Universidade Federal de Goiás, 2025-08-27) Morais, Renata Oliveira Aquino; Carneiro, Megmar Aparecida dos Santos; http://lattes.cnpq.br/8398563469665169; Matos, Márcia Alves Dias de; http://lattes.cnpq.br/0582530071710533; Matos, Márcia Alves Dias de; Souza, Menira Borges de Lima Dias e; Oliveira, Max Moura de
Embargado
Isolamento, caracterização biológica e estabilidade de bacteriófagos líticos e sua compatibilidade com bactérias ácido-láticas (BALs) visando o biocontrole de Salmonella Enteritidis
(Universidade Federal de Goiás, 2025-08-25) Freitas, Daniela Borges; Vieira, José Daniel Gonçalves; http://lattes.cnpq.br/1742731776579730; Vieira, José Daniel Gonçalves; http://lattes.cnpq.br/1742731776579730; Silva, Carla Afonso da; http://lattes.cnpq.br/7673897995590123; Vasconcelos, Lara Stefânia Netto de Oliveira Leão; http://lattes.cnpq.br/5423949965995995
Embargada.
Avaliação histopatológica da glândula digestiva do caramujo Biomphalaria glabrata (Say, 1818) após exposição a nanopartículas verdes
(Universidade Federal de Goiás, 2025-08-28) Ferreira, Luiz Felipe da Silva; Rocha, Thiago Lopes; http://lattes.cnpq.br/6325937100056775; Rocha, Thiago Lopes; http://lattes.cnpq.br/6325937100056775; Rodrigues Filho, Juscelino; http://lattes.cnpq.br/7721100359125364; Tallarico, Lenita de Freitas; http://lattes.cnpq.br/7300603266478611
Embargado
Adenovírus humano em diferentes espécimes clínicos de crianças internadas em unidade de terapia intensiva em Goiânia-Go
(Universidade Federal de Goiás, 2025-08-29) Sousa, José Arthur Silva e; Souza, Menira Borges de Lima Dias e; http://lattes.cnpq.br/0054562567103606; Souza, Menira Borges de Lima Dias e; Matos, Marcia Alves Dias de; Freitas, Erika Regina Leal de
Resumo
Implicações da adesina iceA de Helicobacter pylori para os desfechos clínicos de pacientes dispépticos
(Universidade Federal de Goiás, 2025-06-05) Silva, Mariana Oliveira; Rocha, Viviane Lopes; http://lattes.cnpq.br/7165684017307847; Barbosa, Mônica Santiago; http://lattes.cnpq.br/2417424087790287; Barbosa, Mônica Santiago; http://lattes.cnpq.br/2417424087790287; Santos, Rodrigo da silva; http://lattes.cnpq.br/4806187026900959; Rasmussen, Lucas Trevizani; http://lattes.cnpq.br/9152072043915486
Embargada
Bioprospecção de bactérias de ambientes cavernícola com potencial antimicrobiano
(Universidade Federal de Goiás, 2025-06-26) Lima, Michel Gentile; Vieira, José Daniel Gonçalves; http://lattes.cnpq.br/1742731776579730; Vieira, José Daniel Gonçalves; http://lattes.cnpq.br/1742731776579730; Amaral, André Corrêa; http://lattes.cnpq.br/8801299423520104; Anunciação, Carlos Eduardo; http://lattes.cnpq.br/4354412874919580
Embargado
Toxicidade de nanopartículas de prata funcionalizadas com ácido mercaptosuccínico e químicos ambientais no caramujo Biomphalaria glabrata (Say, 1818)
(Universidade Federal de Goiás, 2024-12-13) Leão, Gabrielly Rodrigues; Silva, Luciana Damacena; http://lattes.cnpq.br/3800305083164308; Rocha, Thiago Lopes; http://lattes.cnpq.br/6325937100056775; Rocha, Thiago Lopes; http://lattes.cnpq.br/6325937100056775; Tallarico, Lenita de Freitas; http://lattes.cnpq.br/7300603266478611; Rodrigues Filho, Juscelino; http://lattes.cnpq.br/7721100359125364
Embargado
Cultivo submerso de Metarhizium anisopliae (Metsch.) Sorokin: Estratégias para a otimização da produtividade e secagem de blastosporos
(Universidade Federal de Goiás, 2025-03-12) Peixoto, Marciel José; Mascarin, Gabriel Moura; http://lattes.cnpq.br/3162686608683670; Fernandes, Éverton Kort Kamp; http://lattes.cnpq.br/2135541732341157; Fernandes, Éverton Kort Kamp; http://lattes.cnpq.br/2135541732341157; Faria, Marcos Rodrigues de; http://lattes.cnpq.br/3612465581571231; Lima, Valesca Henrique; http://lattes.cnpq.br/4870259780815703
Embargado.
Análises do papel do TLR7 em infecções por Leishmania (Viannia) guyanensis ou Leishmania (Viannia) braziliensis
(Universidade Federal de Goiás, 2025-03-07) Torres, Ana Luisa Macedo das Mercez; Dias, Fátima Ribeiro; http://lattes.cnpq.br/5741031258926403; Dias, Fátima Ribeiro; Carmo Neto, José Rodrigues do; Fonseca, Simone Gonçalves da
Embargado.
Galleria mellonella como modelo experimental para avaliar os efeitos nocivos de cocaína: resposta imune e comportamental
(Universidade Federal de Goiás, 2025-02-07) Noleto, Ludmylla Prateado de Assis Costa; Amaral, André Corrêa; http://lattes.cnpq.br/8801299423520104; Amaral, André Corrêa; Garcia, Luane Ferreira; Fernandes, Éverton Kort Kamp
Galleria mellonella (“wax moth”) has been used to study the pathogenesis of microorganisms, the toxicity of antimicrobials and physical and chemical agents. The use of this insect as a model system in animal research is possible because the immune system of G. mellonella shares functional and structural homology with the innate immune system of vertebrates. The present study evaluated the use of G. mellonella larvae as a model organism to investigate the effects and toxicity of cocaine using negative control groups (PBS), positive control groups (DMSO) and test groups with different concentrations of cocaine. A pilot test was carried out with concentrations of 5 mg/mL, 7.5 mg/mL, 10 mg/mL and 20 mg/mL of cocaine and behavioral changes were observed for 120 min. Based on the survival data, concentrations of 10 mg/mL, 20 mg/mL, 40 mg/mL, 80 mg/mL and 200 mg/mL of cocaine were standardized to assess the health of the larvae, the percentage of survival and death and the average lethal concentration (LC50), which were analyzed over 120 h. To assess the immune response and tissue damage, concentrations of 10 mg/mL, 20 mg/mL, 40 mg/mL and 80 mg/mL were used 24 h after drug administration. The behavioral changes observed were: immediate and intermittent hyperexcitation (rhythmic tremors, sweating and regurgitation), followed by paralysis with sporadic spasms and, finally, complete paralysis, not necessarily followed by death. Cocaine did not inhibit silk production and cocoon formation (except at a concentration of 200 mg/mL, where all the individuals died less than 24 hours after administration of the drug). The death of at least one individual was observed in the test groups during the 120 hours of the experiments. The administration of different concentrations of cocaine produced immune responses in G. mellonella larvae: with regard to cellular immunity, activation of granulocytes, spherulocytes and oenocytoids was observed; and with regard to humoral immunity, activation of the pro-phenoloxidase cascade was observed, with subsequent melanization at the highest concentrations of the drug (80 mg/mL and 200 mg/mL) in nearly half of the body surface of the lower region (caudal). There was tissue damage in the digestive system of G. mellonella larvae characterized by a reduction in the haemocyte layer and rupture of part of the intestine (middle or final) which may be related to the administration of different concentrations of cocaine. The LC50 was 73.53 mg/mL. Cocaine had an immunosuppressive and immunotoxic effect on G. mellonella larvae. In conclusion, G. mellonella larvae can be used as a model system for initial studies of the acute effects of cocaine, with the potential for studies of chronic abuse (addiction).
Avaliação do papel da opsonização na suscetibilidade de macrófagos murinos à infecção pelas diferentes espécies de Leishmania
(Universidade Federal de Goiás, 2023-03-31) Polonski, Daniella Cristina Silva; Oliveira, Milton Adriano Pelli de; http://lattes.cnpq.br/2152513705182408; Oliveira, Milton Adriano Pelli de; Gomes, Rodrigo Saar; Borges, Arissa Felipe
Leishmaniasis are a set of parasitic diseases caused by a protozoa of the Leishmania genus. Clinical manifestations vary according to the Leishmania species and host immune response. Among of the possible clinical manifestation of leishmaniasis, according to the symptomatology, three stand out: visceral, cutaneous and mucosal leishmaniasis. Macrophages are key cells in the prognosis of Leishmania infection. M1 macrophages stimulate the Th1 response are associated with leishmanicidal activity; the M2 macrophages are activated mainly by Th2 lymphocytes are associated with the growth and survival of the parasites. The M2b subtype regulates the immune response and the inflammatory reaction against intracellular parasites and is related to the humoral response. The present work evaluated the susceptibility of different species of Leishmania: Leishmania (V.) braziliensis, Leishmania (L.) major, Leishmania (L.) infantum and Leishmania (L.) amazonensis to in vitro infections, using macrophages from BALB/c and C57BL/6. Murine macrophages were cultured and infected with promastigotes from different Leishmania species, opsonized or not with anti-leishmania antibody. The infection was evaluated by the promastigotes retrieval method at the points: 3 h, 24 h, 72h and 120h. We found that C57BL/6 macrophages are resistant to infection by L. (V.) braziliensis and L. (L) amazonensis. BALB/c mice show susceptibility to L. (L) infantum. Both C57BL/6 and BALB/c infected with L. (L.) major parasites show susceptibility to the parasite. Both in infections with L. major and L. infatum, it was observed that the association with antibodies is more related to the reproduction of the parasites and survival inside the macrophages than to the killing of parasites We therefore conclude that opsonization with anti-leishmania can modulate the macrophage to a characteristic M2b profile, favoring infection.
Associação entre polimorfismos genéticos do hospedeiro e suscetibilidade à infecção por Helicobacter pylori: uma revisão sistemática e meta-análise
(Universidade Federal de Goiás, 2024-08-08) Santos, Hellen Christina de Oliveira; Santos, Rodrigo da Silva; http://lattes.cnpq.br/4806187026900959; Barbosa, Mônica Santiago; http://lattes.cnpq.br/2417424087790287; Barbosa, Mônica Santiago; Gomes, Rodrigo Saar; Rocha, Viviane Lopes
Conteúdo embargado
Papel da sinalização purinérgica na infecção por Leishmania braziliensis
(Universidade Federal de Goiás, 2025-02-21) Paula, Wesley Lima de; Gomes, Rodrigo Saar; http://lattes.cnpq.br/8840051460928720; Gomes, Rodrigo Saar; Dias, Fátima Ribeiro; Borges, Pauline Martins Leite
Leishmaniasis are infectious-parasitic diseases caused by protozoa of the genus Leishmania. The infection process causes the release of ATP by cells and tissues. Extracellular ATP can be converted into adenosine by the enzymes CD39 and CD73. Adenosine has anti-inflammatory effects by binding to P1 receptors, especially A2A and A2B receptors. This work evaluated the role of purinergic signaling in the human immune response to Leishmania braziliensis. Data obtained from transcriptome analyzes (GEO GSE55664) show that patients with localized cutaneous leishmaniasis (LCL; n = 21), caused by L. braziliensis, showed higher expression of CD39, CD73 and A2A receptor than healthy controls (n = 7). On the other hand, A2BR expression was lower in patients with LCL. In patients, we found positive correlations between the expression of A2AR and IL1B, IL6 and IL32, while the expression of A2BR is negatively correlated with the expression of the genes of these cytokines. Parasitism rates were evaluated in human THP-1 macrophages infected by Leishmania braziliensis-GFP, which also expressed higher levels of A2AR, in the presence and absence of selective A2AR antagonists, at 24 and 48 hours. There was no change in parasitism rates in macrophages and in the production of cytokines and ROS. However, when the enzyme adenosine deaminase (ADA) is inhibited, the A2AR antagonist significantly reduces infection rates. These data suggest that L. braziliensis infection alters the expression of enzymes and receptors involved in purinergic signaling, and these changes are important in modulating the immune response during LCL.
Análise funcional da 7-desidrocolesterol redutase na resposta imune durante a infecção por Leishmania spp.
(Universidade Federal de Goiás, 2024-02-24) Mota, Carlos Henrique da Silva; Gomes, Rodrigo Saar; http://lattes.cnpq.br/8840051460928720; Rodrigo Saar; Gomes, Rodrigo Saar; Fonseca, Simone Gonçalves da; Silveira, Murilo Barros
Leishmaniasis is caused by protozoa of the genus Leishmania spp. and is a neglected parasitic disease with a broad clinical spectrum, whose treatments are toxic and present failures and resistance. Leishmania braziliensis (Lb) and Leishmania amazonensis (La) are highly prevalent species in Brazil. Cholesterol metabolism affects immune responses. 7-dehydrocholesterol reductase (DHCR7) converts 7-dehydrocholesterol (7-DHC) into cholesterol and this enzyme regulates the polarization of macrophages towards the M2 profile. The main objective is to evaluate the role of DHCR7 in the immune response and in the control of Leishmania spp. infections. Human macrophages derived from THP-1 cells were infected with L. amazonensis or L. braziliensis expressing green fluorescent protein (GFP) for 24 h and treated with pharmacological inhibitors of DHCR7 (tamoxifen and AY9944) for an additional 24 h for flow cytometric analysis. Public transcriptome analysis demonstrated that DHCR7 is less expressed in lesions from patients with cutaneous leishmaniasis compared with skin from healthy controls. DHCR7 is negatively correlated with the expression of pro-inflammatory molecules in lesions from patients. Inhibition of DHCR7 reduced infection by both Leishmania species in human macrophages. Furthermore, DHCR7 inhibitors promoted an increase in the production of reactive oxygen intermediates (ROS) and in the production of TNF and IL-1β by human macrophages infected with Leishmania spp, assessed by CBA in the supernatant. These data suggest that DHCR7 inhibitors control Leishmania spp. infection and pave the way for new therapeutic approaches for this neglected disease.
Atividade biológica de óleos essenciais em leveduras do complexo Cryptococcus neoformans
(Universidade Federal de Goiás, 2013-06-24) Abrão, Fernando Yano; Souza, Lúcia Kioko Hasimoto e; Oliveira, Milton Adriano Pelli de; Paula, José Realino de
Species of the complex Cryptococcus neoformans can cause infection in both healthy individuals and immunocompromissed patients, especially in AIDS patients, in which meningoencephalitis is the main clinical manifestation. There are few available antifungals for cryptococcosis treatment and all of them present high toxicity, besides the reports of resistance. In this context, the natural products are important source in the search for new antifungal compounds. In this study, it was evaluated the biological activity of the essential oils (EO) of Pelargonium graveolensis, Syzygium aromaticum and Cymbopogon flexuosus. The antifungal activity against Cryptococcus isolates was determined by minimum inhibitory concentration (MIC) by microdilution broth. Once the antifungal activity was measured, its cytotoxic activity were evaluated in L929 fibroblastic cell line in an in vitro assay. The mechanism of action of the EOs was analyzed by flow cytometry, using propidium iodide as fluorescent marker for lesions in the cell membrane, and FUN-1 ([2-chloro-4-(2,3-dihydro-3-methyl- (benzo-1,3-thiazol-2-yl)-methylidene)-1-phenylquinolinium iodide]), to analyze alterations of the cellular metabolism. Furthermore, the amount of ergosterol in the fungal membrane was evaluated to ascertain if there were modifications on its synthesis due to the exposition to the EOs. All the EOs presented antifungal activity, P. graveolensis and S. aromaticum with MICs between 128 and 256 µg/mL, while C. flexuosus obtained MICs from 8 to 32 µg/mL, whereas for these values, none EO has shown cytotoxicity in L929 fibroblastic cell line. The metabolic inhibition of fungal cells and membrane lesion were observed as the mechanism of action of all the evaluated EOs, however, only the EO of S. aromaticum was able to decrease the ergosterol amount in the fungal membrane. This study reveals the antifungal potential of the EOs of P. graveolens, S. aromaticum e C. flexuosus, due to their activity in isolated samples of Cryptococcus and relative toxicological safety.

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