Caracterização molecular de isolados de Trypanosoma cruzi obtidos de mulheres durante a fase crônica da doença de Chagas
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2008-02-29
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Universidade Federal de Goiás
Resumo
Chagas disease is a zoonotic disease caused by a protozoan plagued
called Trypanosoma cruzi, it is a particular problem in Latin America, where it is
estimated that 12 to 14 million people are infected. The populations of
Trypanosoma cruzi can be divided into two major groups, the Trypanossoma
cruzi I (TCI) and Trypanossoma cruzi II (TCII), the last being more
heterogeneous and divided into five subgroups TCIIa, TCIIb, TCIIc, TCIId and
TCIIe However, little is known of epidemiological and clinical implications of
these ratings. The frequency of vertical transmission of Chagas disease can
vary from 1.6 to 18.5% in accordance with the region and the methodology used
in the studies. The aim of this study was to genetic characterization of 30
isolates of T. cruzi through the blood of patients with Chagas disease in the
chronic phase, pregnant and not pregnant. The genetic characterization was
performed with three different markers that amplified regions of the 18S and
24S α DNA ribosomal and mini-exon of the gene that allows classify the groups
and subgroups of the parasite and the analysis of genetic variability among
isolates of T. cruzi was performed the characterization of the variable region of
minicicles of kDNA through the technique of Low-Stringency Single Specific
Primer-PCR (LSSP-PCR). The 30 patients studied 75% (25/30) were women
and 25% (5 / 30) not pregnant. The patients are the four different Brazilian
regions being the States of Bahia 53.3% (16/30), Goiás 40.0% (12/30), Paraíba
3.3% (1 / 30) and the Federal District 3, 3% (1 / 30). The analysis of rDNA
allowed show the TCII in 100% of the isolated populations in the regions
studied, where the subgroup TCIIb/e has been detected in (96.66%) and TCIId
in (3.33%) the latter is a sample of the group Control of the State of Bahia. The
genetic variability between the 30 samples was 24.08 ± 14.4% share of bands
among pairs. There was no difference between the share of bands patients of
Bahia and Goiás, where the value of significance was less than 5%. There was
no difference between the percentages of bands showed in analyses by age of
the patients, gestational age and in the analysis of the groups of pregnant and
not pregnant. In reviewing more than one tube of blood per patient coming
evidenced profiles of signatures genomic complex and heterogeneous with
62.5% of bands shared less than 77% and only 37.5% had rates higher than
83% of bands sharing among samples. Suggesting so our isolates showed a
great genetic variability.
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Citação
AVELAR, Juliana Boaventura. Caracterização molecular de isolados de Trypanosoma cruzi
obtidos de mulheres durante a fase crônica da doença de Chagas. 2008. 101 f. Dissertação (Mestrado em Medicina) - Universidade Federal de Goiás, Goiânia, 2008.