Avaliação da expressão da molécula HLA-G no microambiente tumoral e na saliva de pacientes com carcinoma espinocelular de cavidade oral
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Data
2014-01-31
Autores
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Universidade Federal de Goiás
Resumo
HLA-G is a molecule of the major histocompatibility complex (MHC) class I nonclassical that plays an inhibitory effect on immunocompetent cells that are
fundamental in the development of an antitumoral response. In this context, the aim
of this study was to evaluate the expression of HLA-G in the microenvironment of
oral cavity squamous cell carcinoma (OCSCC) as well as their relationship with
clinical and microscopic parameters. In addition, we aimed also to compare the
salivary concentration of soluble HLA-G (sHLA-G) in patients with OCSCC with
healthy individuals (control group). The techniques of immunohistochemistry and
Enzyme- Linked Immunosorbent Assay (ELISA) were used. The results revealed,
with the immunohistochemistry technique, that expression of HLA-G by tumor cells
was significantly higher in metastatic OCSCC (n=30) (72% of samples) when
compared with non-metastatic OCSCC (n=30) (28% of samples) (P=0.01 ). Moreover,
patients with a lower expression of HLA-G presented a tendency to survival longer
(22 months) when compared with those with a higher expression of the molecule (16
months). With reference to other clinicopathological parameters analyzed, only the
presence of lymph node metastasis (P=0.01) and depth of tumor invasion (P=0.02)
were significantly associated with the expression of HLA-G. By ELISA technique, we
showed that the salivary concentration of soluble HLA-G provided no differentiation
between patients with OCSCC from healthy individuals, since there was no
statistically significant difference between the analyzed groups (P=0.17). The findings
of this study revealed that high expression of HLA-G in the microenvironment of
metastatic OCSCC may represent a tumor escape mechanism which contributes to
lower survival and an unfavorable clinical prognosis. However, concentration of
soluble HLA-G in saliva of patients with OCSCC is similar to healthy individuals;
thereby sHLA-G does not represent a salivary biomarker prognostic.
Descrição
Palavras-chave
Neoplasia bucal , Antígenos HLA , Metástase , Saliva , Evasão tumoral , Oral cancer , HLA antigens , Metastasis , Saliva , Tumor escape
Citação
GONÇALVES, A. S. Avaliação da expressão da molécula HLA-G no microambiente tumoral e na saliva de pacientes com carcinoma espinocelular de cavidade oral. 2014. 105 f. Dissertação (Mestrado em Odontologia) - Universidade Federal de Goiás, Goiânia, 2014.