Efeito antibacteriano proporcionado pelo sistema Endox associado a diferentes protocolos de irrigação no preparo de canais radiculares infectados
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2015-03-12
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Universidade Federal de Goiás
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Objectives: To determine the antibacterial effect provided by Endox system
associated with different irrigation protocols in the preparation of infected root canals.
Material and methods: A total of 18 extracted human teeth with anatomical
diameters corresponding to approximately 350-400 micrometers were selected. The
teeth were contaminated with suspension of Enterococcus faecalis (ATCC 29212) for
60 days and randomly divided into five groups: 1. root canal preparation (RCP) +
passive ultrasonic irrigation (PUI) with NaOCl + EDTA; 2. RCP + PUI with NaOCl +
EDTA + Endox system; 3. RCP + conventional irrigation (CI) with NaOCl + EDTA 4.
RCP + CI with NaOCl + EDTA + Endox system; 5. RCP + PUI with distilled water +
EDTA + Endox system. Three teeth uncontaminated were the negative control (group
6). Samples were taken from the root canals before the RCP after 20 minutes and 72
hours, immersed in 7 ml of Brain Heart Infusion (BHI) for a period of 48 hours,
incubated at 37 °C. The presence of bacterial was assessed by turbidity of the
culture medium followed by optical density analysis using UV spectrophotometer.
The differences between groups were statistically analyzed by mean, standard
deviation, variance analysis and Tukey test (post hoc). The significance level was 5%.
Results: The presence of E. faecalis was observed after 72 h in all experimental
groups. In Groups 2 and 4 decreased the optical density of the culture medium after
72 h, with no significant difference between them, while in Groups 1, 3 and 5 was
increased. The average optical density of the culture medium was zero in Group 6.
Conclusion: The Endox system used after the root canal preparation, associated
with conventional irrigation or passive ultrasonic irrigation with NaOCl 2,5% / EDTA,
was not effective in eliminating E. faecalis.
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COELHO, A. Rossi. Efeito antibacteriano proporcionado pelo sistema Endox associado a diferentes protocolos de irrigação no preparo de canais radiculares infectados. 2015. 79 f. Dissertação (Mestrado em Odontologia) - Universidade Federal de Goiás, Goiânia, 2015.