Aditivos nas rações de leitões e seus efeitos no intestino delgado
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2013-12-13
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Universidade Federal de Goiás
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Gut microbiota is changed by various factors, being the diet which has the highest influence,
as well as the modulating additives of the microbiome. The purpose of this work was to
evaluate the effects of growth promoting additives in the diet on the percentage of goblet
cells with acid and neutral mucin in small intestine of pigs, the percent of gut cells in
proliferation by the technique of immunohistochemistry by PCNA, to certify and quantify
the presence of Paneth’s cells by specific staining and by immunohistochemistry, since that
cell has a great importance in the immunity and in the gut microbiota. The experiment was
conducted in a certified swine breeding farm (CSBF), applying the CRD (completely
randomized design). Four hundred and eighty castrated male piglets were used, in five
treatments, with ten piglets by cage, eight repetitions, and in the basal treatment 16
repetitions were used, being T1=basal diet, denominated negative control,
T2=T1+antimicrobial agent, T3=T1+probiotic, Saccharomyces cerevisiae, T4=T1+prebiotic
MOS, T5=T1+probiotic Saccharomyces cerevisiae + the prebiotic MOS. The diet was based
on corn and soybean meal plus a supplement of minerals and vitamins. There were changes
with higher increase in quantity of goblet cells, calculated by millimeter of gut mucosa with
neutral mucus compared to those with acid mucus and to the negative control. There were
no expressive changes for cells with acid mucus. The percentage of proliferating cells in
small intestine by the immunehistochemistry technique for PCNA did not show significant
changes and the presence of Paneth’s cells was confirmed by special staining and by
immunehistochemistry. The quantity of Paneth’s cells of duodenum changed according to
additives used, in comparison with the negative control which had a greater quantity. With
the addition of antimicrobial agent there was a lesser quantity. With the prebiotics and
synbiotics occurred milder reduction and this suggests that immunomodulation occurred.
Besides promoting a better gut health due to a healthier and more appropriate microbiota to
the rearing of piglets in the nursery phase, the addition of growth promoters in feeds favors
significant gains and can be recommended, since the results are compensatory in
productivity.
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KAMIMURA, Regis. Aditivos nas rações de leitões e seus efeitos no intestino delgado. 2013. 100 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2013.