Análise química de extratos de Caryocar brasiliense com potencial antioxidante
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2019-03-01
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Universidade Federal de Goiás
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The pequi (Caryocar brasiliense) is a typical Brazilian fruit that stands out for its high
nutritional value, besides its sensorial attributes like flavor, aroma and color. The fruit peel is
a byproduct of industrial processing and has potential for explotation due to the presence of
phenolic compounds that act in the animal body as antioxidants, capable of preventing or
preventing the action of free radicals that cause degenerative diseases. The objective of this
work was to analyze the presence of phenolic compounds and their antioxidant activities
through chemical analyzes of crude ethanolic extract and its hexane, dichloromethane, ethyl
acetate and aqueous fractions of Caryocar brasiliense bark. The identification of phenolic
compounds was performed by high performance liquid chromatography coupled to high
resolution mass spectrometry. Phenol content was analyzed by determination of the total
phenolic profile by the Folin-Ciocalteu method and the antioxidant capacity was analyzed by
the in vitro antioxidant activity method by the DPPH assay. A total of fifteen phenolic
compounds were identified by comparison with the standards: gallic, ellagic, protocatechic,
gentilic, vanillic, caffeic, ferulic and p-coumaric acids. In addition to catechin, epicatechin,
luteolin, kaempferol, quercetin, rutin and naringerin. The dichloromethane, ethyl acetate and
aqueous fractions showed thirteen of the fifteen phenolic compounds, the crude extract twelve
and the hexane ten. The total phenol profile ranged from 0.6 to 48 mg / 100 mL and the in
vitro antioxidant activity showed high values ranging from 26% to 99.4%. The results confirm
that the crude extract of the bark of the Caryocar brasiliense fruit and its hexane,
dichloromethane, ethyl acetate and aqueous fractions have phenolic compounds and
antioxidant activity.
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FERREIRA, C. M. Análise química de extratos de Caryocar brasiliense com potencial antioxidante. 2019. 133 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2019.